Publications
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Comparative study of the biochemical properties of proteasomes in domestic animals
Where the paper cites Pel-Freez
…as described (Cerruti et al., 2007) with minor modifications. Briefly, samples of muscle and spleen from dog and cat were derived from surgical resections, rabbit muscle and spleen were purchased from Pel Freez Biologicals; mouse muscle and spleen were obtained from animals main- tained in the animal facility unit of the SPAE (University of Torino), in conformity with European laws and poli- cies and with the…
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Brown Adipose Tissue Transplantation Reverses Obesity in Ob/Ob Mice
Where the paper cites Pel-Freez
…immunostaining procedure was used to the detection of tyrosine hydroxylase (TH). Tissues specimens were blocked with 10% normal goat serum for 30 minutes and then incubated with TH antibody (Pel FreeZ, P40101–150) overnight at 4°C, followed by a 1 hour incubation at room temperature with HRP-conjugated goat an- tirabbit IgG. To quantify the size of adipocyte, more than five sections were taken from…
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Developmental validation of the Quantifiler® HP and Trio Kits for human DNA quantification in forensic samples
Where the paper cites Pel-Freez
…Nucleic Acid PrepStation (Thermo Fisher Scientific, Waltham, MA). Whole blood samples of several common animal species (hamster, mouse, rabbit, rat, bovine, pig, sheep and horse) were purchased from Pel-Freez Biologicals (Rogers, AK) and genomic DNA was prepared using the 6100 Nucleic Acid PrepStation. Samples of orangutan and gorilla DNA were obtained as purified genomic DNAs from BIOS Laboratories (New Haven, CT)…
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Actin polymerization is stimulated by actin cross-linking protein palladin
Where the paper cites Pel-Freez
…Purified protein was stored in HEPES buffer at 4 ◦C (20 mM HEPES, pH 7.5, 5 mM dithiothreitol (DTT) and 50 mM NaCl) and used within 2–4 weeks. Actin was purified from rabbit muscle acetone powder (Pel- Freez Biologicals) by using the method of Spudich and Watt [21] and gel-filtered on 16/60 SephacrylTM S-200 column (GE Healthcare Life Sciences). Purified monomeric actin was stored at 4 ◦C in G-buffer (5 mM Tris/HCl,…
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The effect of striatal pre‐enkephalin overexpression in the basal ganglia of the 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine mouse model of Parkinson's disease
Where the paper cites Pel-Freez
…were blocked in solution containing 5% bovine serum albumin, 0.3% Triton X-100 and 10% normal goat serum in PBS for 1 h at room temperature. Sections were then probed for 24 h at 4 °C with rabbit anti-TH (Pel-Freez, Rogers, AR, USA) diluted to 1 : 5000 in PBS solution containing 2% bovine serum albu- min, 0.1% Triton X-100, and 2% normal goat serum. After three washes in PBS, they were incubated for 1 h at room…
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Loss of dopamine phenotype among midbrain neurons inLesch–Nyhan disease
Where the paper cites Pel-Freez
…Membranes were blocked at room temperature in 5% non-fat dry milk in PBS containing 0.05% Tween-20 (PBST). Membranes were then incubated overnight at 4°C with 1:1000 of rabbit anti-TH polyclonal antibody (Pel-Freez, Rogers, AR), 1:500 of rabbit anti-actin polyclonal antibody (Abcam, Cambridge, MA) and 1:1000 of rabbit anti-HPRT1 polyclonal antibody (Aviva Systems Biology, San Diego, CA) in PBST containing 5% dry…
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Determination of 3-mercaptopyruvate in rabbit plasma by high performance liquid chromatography tandem mass spectrometry
Where the paper cites Pel-Freez
…a commercial vendor and a study used to evaluate effectiveness of sulfanegen in cyanide-exposed rabbits. For method development and validation, rabbit plasma (EDTA anti-coagulated) was purchased from Pel- Freeze Biological (Rogers, AR, USA) and stored at −80 ◦C until used. Rabbit plasma from sulfanegen efficacy studies was gathered at the Beckman Laser Institute at the University of California-Irvine. Rabbits…
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Crude and purified proteasome activity assays are affected by type of microplate
Where the paper cites Pel-Freez
…no. 3915) and Corning nonbinding surface (cat. no. 3991) were pur- chased from Corning (Corning, NY, USA). Heart and liver lysate preparation Livers and hearts from 3-month-old rats were obtained from Pel-Freez (Rogers, AR, USA). Tissues were minced with a razor blade and then immediately homogenized using a dounce homog- enizer with 2 ml buffer/0.1 g tissue of 26S buffer (50 mM Tris, 1 mM…
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Anti-lymphoproliferative activity of alpha-2-macroglobulin in the plasma of hibernating 13-lined ground squirrels and woodchucks
Where the paper cites Pel-Freez
…collected in December and February) or 2) was from a non-hibernating, summer-active animal (SA plasma, collected from 2 animals in May and September). Heparinized rat plasma was purchased from PEL Freez Biologicals, Rogers, AR. These plasma samples were aliquoited upon receipt and stored frozen at -80 o C until they were used in experiments. 2.1.3. Mice Female BALB/cByJ mice (5 wks old) were purchased from…
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WAVE Regulatory Complex Activation
Where the paper cites Pel-Freez
…e from freshly slaughtered animals, are chilled and kept on ice immediately after collection, and then processed within 2–3 h. Frozen brains are available from commer- cial sources (e.g., Pel-Freez Biologicals), but we find that results are far more reproducible using brains obtained from a local abattoir. Buffers Extraction buffer 20 mM HEPES, pH 7.4, 0.5 mM ATP, 0.5 mM DTT, 100 mM KCl, 2 mM M
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Transforming growth factor β and platelet-derived growth factor modulation of myofibroblast development from corneal fibroblasts in vitro
Where the paper cites Pel-Freez
…(MSF) under serum-free con- ditions for use in subsequent experiments testing the effects of growth factors or plasmids that produce inhibitory factors on myofibroblast development. Briefly, corneas were removed from Swiss Webster mouse eyes (Pel Freeze, Rogers, AR) and the Descemet’s-endothelium complex was stripped away with 0.12 mm forceps. The remaining stroma and epithelium was incu- bated i
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The reward value of sucrose in leptin-deficient obese mice
Where the paper cites Pel-Freez
…, 140 mM and 0.5 mM [2]. 2.5. Immunohistochemistry Immunohistochemistry was performed following the protocols as published elsewhere [2], using a rabbit anti-TH (Pel-Freez, 1:1000) antibody and appropriate host secondary antibodies. 2.6. Leptin treatment Recombinant mouse leptin was obtained from Amylin Pharmaceuticals (San Diego, CA) and administered through subcutaneous osmotic pumps (Alzet;
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Protein Vicinal Thiol Oxidations in the Healthy Brain: Not So Radical Links between Physiological Oxidative Stress and Neural Cell Activities
Where the paper cites Pel-Freez
…ased from Harlan Laboratories (South Easton, MA). Upon arrival to our facility, animals were euthanized by decapitation and severed heads were dropped immediately into liquid nitrogen. Frozen brains were collected using a chisel. The Reduced and Highly Oxidized brains were obtained from Pel-Freez Biologicals (Rogers, AZ) as custom and standard orders, respectively, and shipped to us on dry ice.
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Neuroprotective effects of mesenchymal stem cells through autophagy modulation in a parkinsonian model
Where the paper cites Pel-Freez
…e incubated overnight at 4 C with primary antibody: mouse anti-tyrosine hydroxylase (TH, 1:2000 dilution for brain tissue, Pel-freez, St. Arkansas, AR, USA). Following this, the brain sections and the cells were rinsed 3 times in 0.5% BSA in 1 PBS (10 min/rinse) and incubated with the appropriate biotinylated secondary antibody and avidinebiotin complex (Elite Kit; Vector Laboratories, Burlin
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MicroRNA-7 Protects against 1-Methyl-4-Phenylpyridinium-Induced Cell Death by Targeting RelA
Where the paper cites Pel-Freez
…RelA J. Neurosci., September 17, 2014 • 34(38):12725–12737 • 12727 Related Disorders at the Banner Sun Health Research Institute. Incuba- tion with rabbit anti-TH antibody (1:500; catalog #P40101-0, Pel-Freez Biologicals) for IF labeling was performed along with anti-digoxigenin- POD antibody. Tissue sections were incubated with anti-rabbit-TRITC secondary antibody (1:1000; catalog #T6778, Sigma) for IF labeling…
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KIR genotype distribution among symptomatic patients with and without Helicobacter pylori infection: is there any role for the B haplotype?
Where the paper cites Pel-Freez
…d for 101 consented Lebanese patients pre- senting to the endoscopy unit and referred for H. pylori testing using the KIR Genotyping SSP kit. We identified 51 H. pylori-positive and 50 H. pylori-negative patients. DNA extraction and KIR genotyping PEL-FREEZ kits (Pel-Freez/Dynal, Norway) were used for DNA extraction from 2 to 3 mL of col- lected peripheral blood. The DNA material was properly la
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Implementation of a Gaussian Beam Laser and Aspheric Optics for High Spatial Resolution MALDI Imaging MS
Where the paper cites Pel-Freez
…(SA), 1,5-diaminonaphthalene (DAN), chloroform, and methanol were obtained from Sigma, and Indium tin oxide (ITO) coated glass slides from Delta Technologies (Loveland, CO, USA). Frozen mouse brain samples, stored at −80°C were purchased from Pel Freez (Rogers, AR, USA). The tissue sections were cut at 10 μm thickness, thaw-mounted on ITO glass slides, and dried in a vacuum desiccator for 30 mi
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Impact of immunization with Protein F on pulmonary clearance of nontypeable Haemophilus influenzae
Where the paper cites Pel-Freez
…of rabbit anti-PF Abs. Briefly, differentiation of HL60 cells into granulocytes was induced by suspending cells into culture medium containing 1.25% dimethyl sulfoxide for 5 days. Baby rabbit complement (Pel-freez Biologi- cals Rogers, AR) was used as a standardized source of complement. Bacteria were pre-incubated with various titers of affinity-purified Abs for 30 min at RT on a mini-orbital shaker. Subsequently,…
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Conditional Knockout of CTGF Affects Corneal Wound Healing
Where the paper cites Pel-Freez
…lace with nail hardener. The pieces of tissue were then imaged with either an epifluorescent or a fluorescent confocal microscope. CTGF ELISA Briefly, frozen, whole, young rabbit globes were purchased from Pel-Freez Biologicals, Inc. (Rogers, AR, USA). The tissues were removed from deep freeze storage and thawed on ice. Each tissue was then grossly dissected from the globe, homogenized, and cleare
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CD147 Required for Corneal Endothelial Lactate Transport
Where the paper cites Pel-Freez
…CAUCUACGAGAAtt-3.0 A scrambled nontargeting siRNA was purchased from Invitrogen (Life Technologies, Grand Island, NY, USA). The transfection reagent HiPerFect was purchased from Qiagen (Pleasanton, CA, USA). New Zealand White rabbit eyes were obtained from Pel- Freez Biologicals (Rogers, AR, USA) and shipped overnight on ice. Corneas were excised with a scleral skirt, and the corneal cup was pl
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Appraisal of the Dopaminergic and Noradrenergic Innervation of the Submucosal Plexus in PD
Where the paper cites Pel-Freez
…a single biopsy was permeabilized for 3 hours in PBS/NaN3 containing 1% (v/v) Triton X- 100 and 10% (v/v) horse serum, and then incubated with the antibodies against tyrosine hydroxylase (TH, 1 : 500, Pel-Freez Biologicals, AR, USA) and neuro- filament 220 kDa (NF, 1 : 250, Millipore, Molsheim, France). Suitable secondary antibodies conjugated to Alexa Fluor 488 and 647 were used (Invitrogen, Ce
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A derivatization and validation strategy for determining the spatial localization of endogenous amine metabolites in tissues using MALDI imaging mass spectrometry
Where the paper cites Pel-Freez
…d MALDI matrices including α-cyano-4- hydroxycinnamic acid (CHCA) and trans-ferulic acid (FA), as well as trifluoroacetic acid (TFA), were obtained from Sigma-Aldrich. Fresh-frozen tissue samples (pig adrenal gland and rat brain) were obtained frozen on dry ice from Pel-Freez Biologicals (Rogers, AR), and subsequently stored at −80°C until use. Pretreatment of MALDI Plates with CHCA for Analysi
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Glutathione S-transferase omega suppresses the defective phenotypes caused by PINK1 loss-of-function in Drosophila
Where the paper cites Pel-Freez
…were dis- sected from 20-day-old flies and fixed with 4% formaldehyde in a fixative buffer. The brains were then stained overnight at 4 C with rabbit anti-tyrosine hydroxylase (TH) antibody (1:100; Pel-Freeze) and mouse anti-TH antibody (1:100; Immunostar). Then, the sam- ples were incubated with Cy3-conjugated secondary antibodies (1:200; Jackson Immunoresearch). The number of DA neurons was counted using a…
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Development of a novel ex vivo porcine skin explant model for the assessment of mature bacterial biofilms
Where the paper cites Pel-Freez
…attached to dermis. Assessment of porcine skin obtained from different sources Both fresh porcine skin and frozen skin, obtained from local (Chiefland Custom Meat, Gainesville, FL) and commercial (Pel-freeze Biologicals, Rogers, AR) sources, were assessed for suitability as biofilm model substrates. The detailed methods used to prepare sheets of porcine skin and significant observations resulting from processing…
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Tuning three-dimensional collagen matrix stiffness independently of collagen concentration modulates endothelial cell behavior
Where the paper cites Pel-Freez
…d for 2 days in the plates prior to embed- ding within collagen gels to allow for spheroid compaction. 2.2. Preparation of collagen gels 2.2.1. Isolation and non-enzymatic glycation of collagen Type I collagen was isolated from rat tail tendons (Pel-Freeze Biologicals, Rogers, AR) and extracted in 0.1% sterile acetic acid (JT Baker, Phillipsburg, NJ) at 4 C. The resulting solution was cen- tri
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Structural requirements for inhibitory effects of bisphenols on the activity of the sarco/endoplasmic reticulum calcium ATPase
Where the paper cites Pel-Freez
…leg tissue for the preparation of microsomes was obtained from Pel-Freez (Rogers, AR). Pyruvate kinase (PK), lactate dehydrogenase (LDH), and Minimum Essential Medium were re- ceived from Sigma–Aldrich (St. Louis, MO) whereas all other re- agents for enzyme activity assays as well as phosphate-buffered saline were from Fisher Scientific (Pittsburgh, PA). Human skin fibroblasts were obtained from
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Stain-Free total protein staining is a superior loading control to β-actin for Western blots
Where the paper cites Pel-Freez
…rocellulose membranes prompted us to determine the suitability of this method as a loading control for Western blots using a tissue (rat liver) commonly used for Western blots. Rat livers (obtained from Pel-Freez, Rogers, AR, USA) were weighed and cut into small pieces with a razor blade and 0003-2697/$ - see front matter 2013 Elsevier Inc. All rights reserved. http://dx.doi.org/10.1016/j.ab.
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Acellular vascular grafts generated from collagen and elastin analogs
Where the paper cites Pel-Freez
…ility. 2. Materials and methods 2.1. Isolation and purification of monomeric Type I collagen Monomeric Type I rat tail tendon collagen was obtained by acid extraction from Sprague–Dawley rats (Pel-Freez Biologicals, Rogers, AR) following a procedure adapted from Silver and Trelstad [28]. Briefly, rat tail tendons were extracted with the aid of auto- claved pliers and dissolved in 10 mM HCl for 4
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A visualized observation of calcium-dependent gelsolin activity upon the surface coverage of fluorescent-tagged actin filaments
Where the paper cites Pel-Freez
…ance, 100 nM of free Ca2+), it binds to F-actin and severing and capping events are followed without an additional Ca2+ binding. 2. Materials and methods 2.1. Actin purification G-actin was purified from a rabbit muscle acetone powder (Pel- freez Arkansas, LLC) by repeated filtration and ultracentrifugation in 1 mM NaHCO3 buffer [23]. G-actin was then polymerized into F-actin in 1 mM NaHCO3 with
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Ultrasound-Enhanced Penetration of Topical Riboflavin Into the Corneal Stroma
Where the paper cites Pel-Freez
…ophoresis enhances drug delivery include radiation forces, acoustic streaming, and acoustic cavitation.17–19 We investigated the ability of phonophoresis to augment the penetration of riboflavin into the corneal stroma over an intact epithelium. METHODS Seventy-two fresh cadaveric rabbit eyes (Pel-Freez Biologicals, Rogers, AR) with intact corneal epithelium were used in this study. The eyes wer