Sheep Anti-Tyrosine Hydroxylase Antibody
Affinity-purified sheep polyclonal antibody. Tested by Western blot on SDS-solubilized human and mouse striatal samples and by IHC on macaque brain sections.
Specifications
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Sheep |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma. |
| Immunogen Species: | Rat |
| Species Reactivity: | Human, Mouse, Rabbit, Rat |
| Gene Name: | TH |
| Molecular Weight: | 60 kDa |
| RRID: | AB_2492277 |
| Purification: | Antigen affinity-purified from pooled serum |
| Processing Method: | Affinity Purified |
| Physical State: | Liquid |
| Buffer: | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50% Glycerol |
| Specificity: | Recognizes endogenous ~60 kDa tyrosine hydroxylase protein. |
| Application Notes: | Recommended starting dilution: WB, IF, IHC 1:1000 |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C Short term, -80°C Long term |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Cold Pack |
| Packaging: | Microtube |
| Expiration: | 1 year from date of receipt when stored at -20°C (short term) or -80°C (long term); aliquot and avoid repeated freeze/thaw. Product quality is guaranteed to meet Pel-Freez Biologicals' specifications for this period when stored as indicated. |
Product Applications
- Western blot
- Immunohistochemistry
- Immunofluorescence (IF/ICC)
- Immunoprecipitation
- Neuroscience research
Documents & Datasheets
Datasheet
Safety Datasheet
Certificate of Analysis
Frequently Asked Questions
What dilution should I use for your antibodies in western blot, ELISA or formalin-fixed paraffin-embedded tissue?
Should I store antibodies at -20°C or -80°C, and should I aliquot them on delivery?
Publications citing this product (3)
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Elevated plasma levels of alpha-synuclein are dispensable for Parkinson’s disease pathology
Where the paper cites Pel-Freez
…ase (1:500, Pel-Freez Biologicals, #P60101-150) and anti-CD31 (1:150, BD Bioscience, #553370). To reduce nonspecific autofluorescence in aged tissue samples, sections were treated with 1× TrueBlack solution (Biotium, #23007) diluted in 70% ethanol for 30 s, followed by thorough PBS washes. Images were acquired with a Leica TCS SP8 confocal microscope (Leica, Germany) and NiKon A1 Rsi/Ti-E (Nikon,
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An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions
Where the paper cites Pel-Freez
…Cruz Biotechnology Sc-990, RRID:AB_2298676 Goat polyclonal anti-OTX2 R&D Systems AF1979, RRID:AB_2157172 Sheep polyclonal anti-PITX2 R&D Systems AF7388, RRID:AB_11128639 Rabbit polyclonal anti-TH Pel-Freez Biologicals P40101-0, RRID:AB_461064 Sheep polyclonal anti-TH Pel-Freez Biologicals P60101-0, RRID:AB_461070 Chemicals, Peptides, and Recombinant Proteins Accutase Innovative Cell Technologies AT104 Ascorbic…
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Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing
Where the paper cites Pel-Freez
…at 100 °C. Proteins were separated with 12% SDS–PAGE and blotted onto a membrane. Blotted membranes were incubated overnight at 4 °C, with an anti-tyrosine hydroxylase antibody (1:1000; P60101-150, Pel-Freez Biologicals). Antibody-reactive bands were then visualized using an enhanced chemiluminscence (ECL) kit (DG-WF200; Dogen). The experimenter was not blinded to the treatment. None of the cell cultures were…
Product References
- Fyn knockdown prevents levodopa-induced dyskinesia in a mouse model of Parkinson’s disease
Bordone, M. P. et al. Fyn knockdown prevents levodopa-induced dyskinesia in a mouse model of Parkinson’s disease. eneuro ENEURO.0559-20.2021 (2021).
- An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions
Gantner, C. W., Cota-Coronado, A., Thompson, L. H. & Parish, C. L. An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions. STAR Protoc. 1, 100065 (2020).
- β‑Lapachone ameliorates L‑DOPA‑induced dyskinesia in a 6‑OHDA‑induced mouse model of Parkinson's disease
Ryu, Y. K. et al. β-Lapachone ameliorates L-DOPA-induced dyskinesia in a 6-OHDA-induced mouse model of Parkinson’s disease. Mol. Med. Rep. 23, (2021).
- Splenic artery loops: Potential splenic plexus stimulation sites for neuroimmunomodulatory‐based anti‐inflammatory therapy?
Cleypool, C. G. J., Lotgerink Bruinenberg, D., Roeling, T., Irwin, E. & Bleys, R. L. A. W. Splenic artery loops: Potential splenic plexus stimulation sites for neuroimmunomodulatory-based anti-inflammatory therapy? Clin. Anat. 34, 371–380 (2021).
- Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing
Park, H., Shin, J., Choi, H., Cho, B. & Kim, J. Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing. Cells 9, (2020).
- Neuroprotection and immunomodulation of progesterone in the gut of a mouse model of Parkinson's disease
Jarras, H. et al. Neuroprotection and immunomodulation of progesterone in the gut of a mouse model of Parkinson’s disease. J. Neuroendocrinol. 32, (2020).
Alternate Names
TH; TYH; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase
