Mahfouz et al. (2014). Journal of Clinical Pathology. doi: 10.1136/jclinpath-2014-202638
…d for 101 consented Lebanese patients pre- senting to the endoscopy unit and referred for H. pylori testing using the KIR Genotyping SSP kit. We identified 51 H. pylori-positive and 50 H. pylori-negative patients. DNA extraction and KIR genotyping PEL-FREEZ kits (Pel-Freez/Dynal, Norway) were used for DNA extraction from 2 to 3 mL of col- lected peripheral blood. The DNA material was properly la
Abstract
Contact of peripheral blood lymphocytes with Helicobacter pylori was proved to induce non- major histocompatibility complex-restricted cytotoxicity and natural killer cells are thought to play an important role in the immunity against H. pylori . Aims In this research, we investigated any possible association between killer immunoglobulin-like receptors (KIR) genotypes and H. pylori infection. Methods KIR genotype was analysed in 101 Lebanese symptomatic patients (51 H. pylori positive and 50 H. pylori- negative) using the KIR Genotyping SSP kit. Results Among the H. pylori -positive patients, the AA, AB and BB genotypical frequencies were, respectively, 43.14%, 41.18% and 15.68% with an A:B ratio of 1.76:1. The AA, AB and BB genotypes frequencies for H. pylori -negative individuals were 18%, 62% and 20%, respectively, with an A:B ratio of 0.96:1. No significant difference between patients and controls was detected. Conclusions We noticed a reduced distribution of A haplotype among the ‘ H. pylori -negative’ patients as compared with the “ H. pylori -positive” group. This is the first study in the international literature that targets the correlation between KIR genotypes and H. pylori .