Normal Human Serum and Plasma

Advance your studies with single-donor and pooled fully-consented human blood products for research use.

15 of 15 Results

5 peer-reviewed papers that used a product from this category, newest first.

All publications
  1. 2026Chanda et al. (2026). bioRxiv (Cold Spring Harbor Laboratory).

    An mRNA–Lipid Nanoparticle Platform Encoding the Conserved Outer Membrane Protein BamA Elicits Broadly Cross-Reactive Systemic and Mucosal Antibodies Against Antimicrobial-Resistant Neisseria gonorrhoeae

    Where the paper cites Pel-Freez

    …362 Ng WHO X (1×10³ CFU in 40 µL HBSS) were added to wells containing diluted test sera 363 and incubated for 15 min at 37°C. Subsequently, 10 µL of either IgG/IgM-depleted normal human serum (NHS; Pel-Freeze Biologicals; Cat. # 34010-10, Lot 34943) or heat- inactivated NHS (HI-NHS; Cat. #34020-10, Lot 13844) was added as a 10% (v/v) complement source, and reactions were incubated for 1 h at 37°C. A single lot of…

  2. 2026Ali et al. (2026). International Journal of Infectious Diseases.

    Do the protein-based meningococcal group B vaccines, 4CMenB and MenB-fHbp, elicit serum bactericidal antibody against meningococcal serogroup X clonal complex 181?

    Where the paper cites Pel-Freez

    …growth CHBA plate after a 4-h incubation. A volume of 10 µL of the bacterial suspension was added to each well of the microtiter plate. A volume of 10 µL of “active” antibody depleted human complement (Pel-Freeze, Inc., Brown Deer, WI) was added to all wells except the sample control wells for viable cfu and sample non-complement mediated lysis control, to which heat-inactivated antibody depleted human complement…

  3. 2025Fegan et al. (2025). npj Vaccines.

    Rational selection of TbpB variants yields a bivalent vaccine with broad coverage against Neisseria gonorrhoeae

    Where the paper cites Pel-Freez

    …allow opsonization, ~500 CFUs were incubated with various concentrations (1:20, 1:40, 1:80, 1:200, 1:400) of serum for 20 min, followed by the addition of 12% pooled human complement depleted of IgG/IgM (Pel Freez, cat. 34010, Lot 22344) and incubated for 60 min at 37 °C with 5% CO 2 . The highest dilution at which 50% killing was observed relative to no antibody control in 60 min was reported as the SBA titer.…

  4. 2024Reveille et al. (2024). Vaccine.

    Development and validation of a standardized human complement serum bactericidal activity assay to measure functional antibody responses to Neisseria gonorrhoeae

    Where the paper cites Pel-Freez

    …strain lacking pora expression and expressing a hybridized porb (och) [18 ]. see table s3 for a complete description of pc reagents. 3.4 intrinsic sba growth assay igg/igm-depleted human complement (c′) pooled serum (pel-freez validation lot #03642) was screened for endogenous killing activity against ng strains f62, ms11, and fa1090 as previously described [19 ] with modifications. briefly, sialylated or non-sial…

  5. 2016Yun et al. (2016). Microbial Pathogenesis.

    Serum bactericidal assay for the evaluation of typhoid vaccine using a semi-automated colony-counting method

    Where the paper cites Pel-Freez

    …4-nitrophenyl phosphate disodium hexahydrate were purchased from Sigma-Aldrich (St. Louis, MO). Baby rabbit (3- to 4-week-old) and adult rabbit (8- to 12-week-old) complements were purchased from Pel-Freez Biologicals (Rogers, AR). Guinea pig and human complements were purchased from Rockland (Gilbertsville, PA) and Valley Biomedical (Winchester, VA), respectively. All complements were stored in aliquots at −80…

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