Domingos et al. (2014). Molecular Metabolism. doi: 10.1016/j.molmet.2013.10.007
…, 140 mM and 0.5 mM [2]. 2.5. Immunohistochemistry Immunohistochemistry was performed following the protocols as published elsewhere [2], using a rabbit anti-TH (Pel-Freez, 1:1000) antibody and appropriate host secondary antibodies. 2.6. Leptin treatment Recombinant mouse leptin was obtained from Amylin Pharmaceuticals (San Diego, CA) and administered through subcutaneous osmotic pumps (Alzet;