Publications
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TWIK-1 plays distinct roles in spinal and peripheral sensory circuits controlling mechanical sensitivity and neuropathic hypersensitivity
Where the paper cites Pel-Freez
…No. 019-19741), goat anti-CGRP (1:1000, Abcam, Cat. No. ab36001), rabbit anti-CSF1R (1:100, Abcam, Cat. No. ab183316), chicken anti-NF200 (1:500, Aves labs, Cat. No. AB_2313552), rabbit anti-TH (1:1000, Pel-Freez, Cat. No. P40101- 150), and rabbit anti-mCherry (1:1000, Abcam, Cat. No. ab167453). The secondary antibodies used were Alexa Fluor-conjugated donkey anti-rabbit (1:500, Life Technologies, Cat. No.…
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Mechanism of actin thin filament pointed-end elongation by leiomodin
Where the paper cites Pel-Freez
…facing actin in the structures (Supplementary Fig. 9). Methods Proteins for cryo-EM analysis Skeletal α-actin54 and Tpm1.155 were purified from fresh hind leg rabbit muscle tissue purchased from Pel-Freeze Biologicals. Full-length human Lmod2 was obtained as we have previously described18. Briefly, the gene was cloned between the NdeI and SapI sites of vector pTYB1 (NEB), which encodes a chitin-binding domain for…
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Loss of PINK1 causes age-dependent mitochondrial trafficking deficits in nigrostriatal dopaminergic neurons via aberrant p38 MAPK activation
Where the paper cites Pel-Freez
…an investigator blinded to the genotype. ARTICLE IN PRESS ARTICLE IN PRESS Antibodies and Reagents The antibodies and reagents used in the study include: Anti-Tyrosine Hydroxylase (rabbit, 1:5000 for IF, Pel-Freez, P40101-0), Anti-Tyrosine Hydroxylase (mouse, 1:500 for IF, Millipore, MAB5280), Anti-Kinesin 5B heavy chain (mouse, 1:500 for WB, Millipore, MAB1614), Anti- Dynein medium chain (mouse, 1:1000 for WB,…
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Leiomodin 2 is a processive pointed-end elongator of actin filaments
Where the paper cites Pel-Freez
…coordinated in cells. Methods Purification and labeling of actin Rabbit skeletal muscle actin was purified from acetone powder gen- erated from frozen ground hind-leg muscle tissue of young rabbits (Pel-Freez Biologicals). Lyophilized acetone powder stored at −80 °C was mechanically sheared in a coffee grinder, resuspended in G-buffer (5 mM Tris-HCl, pH 7.8, 0.5 mM dithiothreitol, 0.2 mM ATP, and 0.1 mM CaCl₂), and…
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High molecular weight insoluble parkin in the substantia nigra of patients with idiopathic Parkinson’s disease
Where the paper cites Pel-Freez
…1 h at ambient temperature. As for the immunoblots, the antibodies against parkin C-Terminal region (monoclonal parkin clone [PRK8], 1:1,000 and polyclonal parkin (cell signaling cat#2132, 1:1,000)), TH (Pel-Freez Biolo- gicalsCat# P40101-150, 1:1,000),αsyn(SYN1,BDBiosciencesCat# 610787, 1:1,000), MJFR1 (Abcam Cat# ab138501 [MJFR1], 1:1,000) and αsyn phosphorylatedatserine129(αsynP129)(AbcamCat#ab168381[MJF-R13…
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Broadly conserved protective epitopes on the lyme disease vaccine antigen, OspA
Where the paper cites Pel-Freez
…burgdorferi HB19-R1 reporter strains. Complement-dependent bactericidal assays were performed using HB19-R1 reporter strains and mAb 857-2 with either 2.5% guinea pig complement or 5% human complement (Pel-Freeze), under conditions described in the materials and methods section. Data shown encompasses 3–5 independent experiments. Differences in strain susceptibility to mAb 857-2 between complement sources were…
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Bitesize bundles F-actin and influences actin remodeling in syncytial Drosophila embryo development.
Where the paper cites Pel-Freez
…Inc). Purification and Labeling of Actin Rabbit skeletal muscle actin was purified from acetone powder (Spudich and Watt, 1971) generated from frozen ground hind leg muscle tissue of young rabbits (PelFreez). Lyophilized acetone powder stored at -80°C was mechanically sheared in a coffee grinder, resuspended in G-buffer (5 mM Tris-HCl [pH 7.5], 0.5 mM Dithiothreitol [DTT], 0.2 mM ATP, 0.1 mM CaCl2), and then…
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An mRNA–Lipid Nanoparticle Platform Encoding the Conserved Outer Membrane Protein BamA Elicits Broadly Cross-Reactive Systemic and Mucosal Antibodies Against Antimicrobial-Resistant Neisseria gonorrhoeae
Where the paper cites Pel-Freez
…362 Ng WHO X (1×10³ CFU in 40 µL HBSS) were added to wells containing diluted test sera 363 and incubated for 15 min at 37°C. Subsequently, 10 µL of either IgG/IgM-depleted normal human serum (NHS; Pel-Freeze Biologicals; Cat. # 34010-10, Lot 34943) or heat- inactivated NHS (HI-NHS; Cat. #34020-10, Lot 13844) was added as a 10% (v/v) complement source, and reactions were incubated for 1 h at 37°C. A single lot of…
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An Fc-silent OspA monoclonal antibody passively protects mice from tick and intradermal Borrelia burgdorferi challenge
Where the paper cites Pel-Freez
…Plates were washed twice using a plate magnet and 190 μL of wash buffer (PBS, 2% BSA, 0.02% Tween-20, 0.05% sodium azide, pH 7.4). Following the washes, 200 μL of diluted human complement (1:50; Pel-Freez Biologicals, Rogers, AR) were added to each well and incubated for 20 min at RT with shaking. Plates were washed again then phycoerythrin-tagged mouse anti-C3/C3b/iC3b (1:100; BD) and phycoerythrin-tagged goat…
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A circular RNA vaccine induces durable and cross-protective immunity against Neisseria meningitidis serogroup B in mice
Where the paper cites Pel-Freez
…N. meningitidis was suspended in Hanks’ balanced salt solution (Gibco, Suzhou, China) supplemented with 0.5% BSA and 20 U/mL heparin sodium to a final concentration of 1.25 × 104 CFU/mL. Human complement (Pel-Freez, Rogers, Arkan sas) was diluted with SBA buffer mentioned above to a final dilution of 1/4. Sera, pooled or from individual mice, were heat inactivated for 30 min at 56°C and added to the wells in a…
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Targeting reverse electron transport preserves mitochondrial function and prevents dopaminergic neurodegeneration in Parkinson’s disease
Where the paper cites Pel-Freez
…then incubated at 4 °C overnight with a rabbit polyclonal primary antibody against TH, GFAP or IBA-1 in KPBS containing 0.4% triton X-100 (TH, Pel Freez #P40101- 150, 1:1000; GFAP, Proteintech #16,825–1-AP 1:500; and Iba-1, Novus biologicals Cat#NBP2-19,019, 1:500). After five to six rinses in KPBS, the sections were treated for two hours in a biotinylated secondary antibody (PK- 6200, 1:1000,
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Miro1 mutations disrupt cellular calcium homeostasis via dysregulation of mitochondria-ER-contact-sites, rendering iPSC-derived neurons more susceptible to lipid peroxidation
Where the paper cites Pel-Freez
…Peprotech, 450-10-50UG). Neuronal cultures were assessed by immunostaining with antibodies against Mikrotubuli-associated protein 2 (MAP2; Abcam ab5392, dilution 1:10000) and Tyrosin Hydroxylase (TH; Pel-Freez P40101, dilution 1:1000). 2.2 Live cell imaging of mitochondrial calcium Neurons were cultivated in 8 well chamber slides (Ibidi, 80806) at 100,000 cells per well. Then, neurons were transfected with 0.5…
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Integration of cross-species multi-omics with in vivo experimental validation identifies Parkinson's disease therapeutic targets and novel risk factors within endolysosomal pathway subnetworks
Where the paper cites Pel-Freez
…times for 5 min each using 1× PBST. Samples were placed in blocking buffer (1% bovine serum albumen in PBST) for 1 h at room temperature and then incubated with anti-Tyrosine Hydroxylase (1:500, P40101 PelFreez) for 3 days at 4 °C. After the primary was removed, samples were washed 3 times with 1× PBST for 5 min each, then incubated in secondary (1:500 Alexa Fluor anti-Rabbit 555 (A-21428) and 1:1000 DAPI) for 1…
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Do the protein-based meningococcal group B vaccines, 4CMenB and MenB-fHbp, elicit serum bactericidal antibody against meningococcal serogroup X clonal complex 181?
Where the paper cites Pel-Freez
…growth CHBA plate after a 4-h incubation. A volume of 10 µL of the bacterial suspension was added to each well of the microtiter plate. A volume of 10 µL of “active” antibody depleted human complement (Pel-Freeze, Inc., Brown Deer, WI) was added to all wells except the sample control wells for viable cfu and sample non-complement mediated lysis control, to which heat-inactivated antibody depleted human complement…
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Development of an 8-valent Salmonella and Shigella MAPS vaccine
Where the paper cites Pel-Freez
…MA, USA). AddaS03, R848 and CpG were purchased from InvivoGen (San Diego, CA, USA). Baby rabbit complement was obtained from Pel-Freez Biologicals (Rogers, AR, USA). All other reagents were obtained from Sigma (St. Louis, MO, USA). Vaccine preparation and immunization procedures The Salmonella and Shigella MAPS vaccines were manufactured as described previously (18, 20). Briefly, OSP and Vi were
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Antiphospholipid antibodies in acute and post-treatment Lyme disease
Where the paper cites Pel-Freez
…id (αPA) and phosphatidylserine (αPS) were measured at study enrollment, within 1 week of initial diagnosis. Samples from two biobanks were compared: 54 from Johns Hopkins University (JHU; the combined day 0 samples from Fig. 3 ) and 64 from the Lyme Disease Biobank (LDB). Controls were from individuals in endemic areas (48 samples from the LDB) and non-endemic areas (45 samples from Pel-Freez)
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A novel rat model of body first subtype of Parkinsons disease
Where the paper cites Pel-Freez
…for 1 hour at room temperature to block nonspecific binding sites and then incubated in primary antibody (rabbit anti- tyrosine hydroxylase, Pel Freez Biologicals #P40101-150, 1:250 in blocking solution) for 72 hours at 4oC on a shaker. Sections were washed in between incubation steps with 6 x 3min PBS-Tx. Sections were incubated in diluted secondary antibody (biotinylated donkey anti-rabbit Ig
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Signal Transduction Pathway Mediating Carotid Body Dependent Sympathetic Activation and Hypertension by Chronic Intermittent Hypoxia
Where the paper cites Pel-Freez
…CM1900), and mounted on collagen-coated coverslips. Sections were blocked in PBS containing 1% normal goat serum and 0.2% Triton X-100 and then incubated with monoclonal rabbit anti-TH antibody (Pel-Freez Biologicals; #P40101-0; dilution 1:4000), followed by 5 washes with PBS containing 0.05% Triton X-100. Antibody binding was detected using Texas red-conjugated goat anti-mouse IgG diluted 1:250 in PBS…
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RNAi-based screen for pigmentation in Drosophila melanogaster reveals regulators of brain dopamine and sleep
Where the paper cites Pel-Freez
…the critical analysis of the article. Declaration of interests The authors declare no competing interests. STAR★Methods Key resources table REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-TH Pel-Freez Biologicals P40101, RRID:AB_461064 anti-elav DSHB 7E8A10, RRID:AB_528218 anti-rat-A488 Thermo Fisher Sci A-21208, RRID:AB_2535794 anti-rabbit-A647 Thermo Fisher Sci A-27040, RRID:AB_2536101 Chemicals, peptides,…
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Resonant acoustic rheometry for assessing plasma coagulation in bleeding patients
Where the paper cites Pel-Freez
…10 min for RAR assays. Each plasma sample was divided into nine equal portions for nine RAR reagent conditions that contain different concentrations of CaCl 2 (Fluka), Rabbit Brain Cephalin (Lipids, Pel-Freez Biologicals), kaolin (Fisher Scientific), TF stock (10× Dade Innovin, Siemens), thrombin (Fisher Scientific) and 0.85% buffered saline (Fisher Scientific). The nine reagent conditions are: (1) Control (calcium…
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Rational selection of TbpB variants yields a bivalent vaccine with broad coverage against Neisseria gonorrhoeae
Where the paper cites Pel-Freez
…allow opsonization, ~500 CFUs were incubated with various concentrations (1:20, 1:40, 1:80, 1:200, 1:400) of serum for 20 min, followed by the addition of 12% pooled human complement depleted of IgG/IgM (Pel Freez, cat. 34010, Lot 22344) and incubated for 60 min at 37 °C with 5% CO 2 . The highest dilution at which 50% killing was observed relative to no antibody control in 60 min was reported as the SBA titer.…
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Protocol for differentiating human pluripotent stem cells into midbrain organoids for targeted microinjection of viruses
Where the paper cites Pel-Freez
…74376 NESTIN (dilution 1:1,000) Santa Cruz Cat#sc-21247 OTX2(dilution 1:1,000) R&D Systems Cat# AF2018 DCX (dilution 1:1,000) CST Cat#4604 SOX2 (dilution 1:500) R&D Systems Cat#AF2018 TH (dilution 1:1,000) Pel-Freez Cat#P40101 GAD67 (dilution 1:1,000) Millipore Cat#MAB5406 Donkey anti-mouse Alexa Fluor 488 (dilution 1:1,000) Thermo Fisher Scientific Cat#A21202 Donkey anti-rabbit Alexa Fluor 488 (dilution 1:1,000)…
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Preclinical Profile of CM699 as a Medication Candidate for Stimulant Use Disorder
Where the paper cites Pel-Freez
…cation candidatefor stimulant use disorder … frozen brains from male sprague–dawley rats weighing 200–225 g (taconic biosciences co., germantown, ny, usa) were used for monoamine transporter assays, whereas frozen whole guinea pig brains excluding cerebella ( pel - freez…
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Perspective Chapter: Potassium Regulation in Pregnancy and Pre-Eclampsia – The Identity and Role of Non-Genomic Steroids
Where the paper cites Pel-Freez
…a local slaughterhouse near San Francisco. Six compounds were isolated to near purity. No SPCDs with 24 carbon atoms were identified. More recently, we isolated SPCDs from bovine adrenals obtained from Pel-Freeze, Little Rock, AK, at Coddle Creek Laboratories. These samples included SPCDs with both 23 carbon atoms and 24 carbon atoms. This discrepancy in isolated SPCDs was attributed to the endocrinological and…
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Neurotherapeutic effects of Vutiglabridin as a Paraoxonase-2 modulator in preclinical models of Parkinson’s disease
Where the paper cites Pel-Freez
…d dilution and the sec tions were incubated in a shaker at 4 °C overnight. Pri mary antibodies used for immunostaining were anti-TH (rabbit, 1:200, Pel-freez, P40101-150), GFAP (chicken, 1:500, Millipore, AB5541), Iba-1 (rabbit, 1:500, Wako, 019-19741), DDC (rabbit, 1:200, abCam, ab3905), GABA (guinea pig, 1:200, Millipore, AB175), anti-PON2 (rab bit, 1:200, abCam, ab183710). Unbound antibod
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Myosin-based nucleation of actin filaments contributes to stereocilia development critical for hearing
Where the paper cites Pel-Freez
…Technologies) in blocking buffer (2% NGS in PBS). Confocal microscopy images were captured as described above. Actin purification and labelling Actin was extracted from rabbit skeletal acetone powder (Pel-Freeze, AZ) in chilled G-buffer (concentrations in mM), Tris-HCl (2), ATP (0.2), CaCl 2 (0.1), NaN 3 (1), DTT (1), pH 8, using established protocols 122 . Actin was additionally labelled on Cys 374 using either…
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Monkeypox virus H3L protein as the target antigen for developing neutralizing antibody and serological assay
Where the paper cites Pel-Freez
…CO2 incubator. On the day for performing the viral infection step, MPXV (50 PFU) was mixed with antibody sample in the absence or presence of Applied Microbiology and Biotechnology (2025) 109:80 80 Page 4 of 12 10% baby rabbit complements (31,061–1, Pel-Freez, Rogers, AR, USA) at 37 °C for 1 h. The mixture was then added to the Vero E6 cell monolayer seeded in the well of the 24-well plate and
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Molecularly distinct striatonigral neuron subtypes differentially regulate locomotion
Where the paper cites Pel-Freez
…Antifade Mountant, Life technology). Images were taken using a laser scanning confocal microscope LSM 780 (Zeiss). The primary antibodies used for immunostaining included rabbit monoclonal anti-TH (Pel-Freez Biologicals, P40101; dilution 1:1000), mouse monoclonal anti-TH (ImmunoStar, 22941; dilution 1:1000), chicken polyclonal anti-TH (Aves Labs, TYH; dilution 1:500), rabbit anti-Mu-opioid receptor (MOR)…
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Microglia depletion reduces neurodegeneration and remodels extracellular matrix in a mouse Parkinson’s disease model triggered by α-synuclein overexpression
Where the paper cites Pel-Freez
…30 min to eliminate endogenous oxidase. The samples were then blocked with 5% normal goat serum (ZSGB-Bio, Cat#: ZLI-9021, Lot#: 190320) for 1 h at room temperature, then incubated with rabbit anti-TH (Pel-Freez, Cat#: P40101, Lot#: aj0520P, 1:1000) or rabbit anti-NeuN (EMD Millipore Corp, Cat#: ABN78, Lot#: 3692639, 1:500) overnight. On the second day, the brain slices were incubated with a secondary antibody…
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Mannoprotein Cig1 contributes to the immunogenicity of a heat-killed F-box protein Fbp1 Cryptococcus neoformans vaccine model
Where the paper cites Pel-Freez
…at 37°C in activating medium (DME medium, 50 U/mL of IFN- γ, 1 g/mL of lipopolysaccharide [LPS]). Phosphate-buffered saline pH 7.4 (PBS)-washed fungal cells were opsonized with 20% mouse complement (Pel-Freez, AK) and added to the macrophages at an effector-to-target ratio of 1:2. Phagocytosis was allowed to occur for 2 h at 37°C in 10% CO 2 . The cells were then washed three times with 1X PBS and fixed with…