Famouri et al. (2013). Journal of Colloid and Interface Science. doi: 10.1016/j.jcis.2012.08.049
…ance, 100 nM of free Ca2+), it binds to F-actin and severing and capping events are followed without an additional Ca2+ binding. 2. Materials and methods 2.1. Actin purification G-actin was purified from a rabbit muscle acetone powder (Pel- freez Arkansas, LLC) by repeated filtration and ultracentrifugation in 1 mM NaHCO3 buffer [23]. G-actin was then polymerized into F-actin in 1 mM NaHCO3 with