Human Blood Product Categories
Pel-Freez specializes in customization. If you don’t see what you need please contact us, we can customize existing products, perform additional processing or analyses, and even custom source a product to keep your project moving forward.
Popular Human Blood for Research
Applications
Vaccine Development
Well known and widely used in the vaccine industry, Pel-Freez enables a range of safety and efficacy assays including:
- Complement-dependent cytotoxicity assays (CDC)
- Opsonophagocytic killing assays (OPKA)
- Serum bactericidal assays (SBA)
With the ability to deliver bulk scales of consistently-performing human complement >97% depleted of background killing antibodies through our proprietary process, we can spare your teams the hassle of preparing your own complement, maximizing efficiency and productivity.
Assay Development & Validation
In addition to advancing vaccine development, Pel-Freez’s human blood products for research can support development and validation of a range of assays.
You’ll find serum and plasma from healthy donors, available as pooled or individual donor samples, for fueling the development and validation of assays that analyze human blood.
And with ready-to-use complement, antibody depleted serum, and immunoglobulins, you’ve got reliable components for vaccine safety and efficacy assays and more.
In Vitro Diagnostics (IVDs)
Our human blood products can also support development of IVDs that test for or rely on plasma, serum, or immune components.
All serum and plasma is ethically obtained, fully consented and collected under IRB approved protocols, and ready for use in your IVD development program.
All human blood products start with the same ethically collected serum and plasma and are further processed in our facility using tightly controlled and highly standardized workflows. As a result, you can be confident that the consistency, quality, and reliability of our materials can be counted on to advance your IVD program.
Frequently asked questions
How is Pel-Freez Biologicals' antibody depleted complement made?
We have developed a proprietary process for antibody removal based on affinity chromatography. Our method results in low residual antibody (<3% vs. feed serum) while maintaining high complement potency.
What's the difference between Pel-Freez Biologicals' antibody depleted human complement (ADHC) and antibody depleted human serum (ADHS)?
ADHC retains high complement potency for use in assays requiring functional complement activity. ADHS is devoid of complement activity due to heat-inactivation and thus can be used in applications requiring serum components other than complement, e.g. a negative matrix for dilution, preparation of QC samples, or cell culture applications where a nutrient source is need but complement function could inhibit cell growth.
Should I use human, rabbit, or guinea pig complement for my application?
Work backwards from the readout. Complement regulators are host-specific, so the complement source is part of the assay method, not a preference.
- Antibody-depleted human complement for the hSBA (serogroup B meningococcus and gonococcus; correlate hSBA ≥ 1:4). Neisseria recruits human factor H, which rabbit and guinea pig factor H cannot replace, and the donor antibodies in native human serum would kill the target on their own.
- Baby rabbit complement for the rSBA (serogroups A, C, W and Y; correlate rSBA ≥ 1:8) and for pneumococcal OPA/MOPA, which was standardized on it.
- Adult rabbit complement for complement-dependent cytotoxicity and HLA crossmatching, the classical cell-lysis source.
- Guinea pig complement where potency per volume is the point: CH50 titration, complement fixation and the vibriocidal assay.
When the model animal's own cascade is the readout, use matched-species complement. Mouse, rat, pig, canine, cynomolgus, goat and hamster complement are available on request through our custom quote form.
For what the research literature actually uses, by pathogen and assay, see which complement source for which assay.
Research citing Human Blood for Research
5 peer-reviewed papers that used a product from this category, newest first.
All publications-
An mRNA–Lipid Nanoparticle Platform Encoding the Conserved Outer Membrane Protein BamA Elicits Broadly Cross-Reactive Systemic and Mucosal Antibodies Against Antimicrobial-Resistant Neisseria gonorrhoeae
Where the paper cites Pel-Freez
…362 Ng WHO X (1×10³ CFU in 40 µL HBSS) were added to wells containing diluted test sera 363 and incubated for 15 min at 37°C. Subsequently, 10 µL of either IgG/IgM-depleted normal human serum (NHS; Pel-Freeze Biologicals; Cat. # 34010-10, Lot 34943) or heat- inactivated NHS (HI-NHS; Cat. #34020-10, Lot 13844) was added as a 10% (v/v) complement source, and reactions were incubated for 1 h at 37°C. A single lot of…
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Do the protein-based meningococcal group B vaccines, 4CMenB and MenB-fHbp, elicit serum bactericidal antibody against meningococcal serogroup X clonal complex 181?
Where the paper cites Pel-Freez
…growth CHBA plate after a 4-h incubation. A volume of 10 µL of the bacterial suspension was added to each well of the microtiter plate. A volume of 10 µL of “active” antibody depleted human complement (Pel-Freeze, Inc., Brown Deer, WI) was added to all wells except the sample control wells for viable cfu and sample non-complement mediated lysis control, to which heat-inactivated antibody depleted human complement…
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Rational selection of TbpB variants yields a bivalent vaccine with broad coverage against Neisseria gonorrhoeae
Where the paper cites Pel-Freez
…allow opsonization, ~500 CFUs were incubated with various concentrations (1:20, 1:40, 1:80, 1:200, 1:400) of serum for 20 min, followed by the addition of 12% pooled human complement depleted of IgG/IgM (Pel Freez, cat. 34010, Lot 22344) and incubated for 60 min at 37 °C with 5% CO 2 . The highest dilution at which 50% killing was observed relative to no antibody control in 60 min was reported as the SBA titer.…
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Development and validation of a standardized human complement serum bactericidal activity assay to measure functional antibody responses to Neisseria gonorrhoeae
Where the paper cites Pel-Freez
…strain lacking pora expression and expressing a hybridized porb (och) [18 ]. see table s3 for a complete description of pc reagents. 3.4 intrinsic sba growth assay igg/igm-depleted human complement (c′) pooled serum (pel-freez validation lot #03642) was screened for endogenous killing activity against ng strains f62, ms11, and fa1090 as previously described [19 ] with modifications. briefly, sialylated or non-sial…
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Serum bactericidal assay for the evaluation of typhoid vaccine using a semi-automated colony-counting method
Where the paper cites Pel-Freez
…4-nitrophenyl phosphate disodium hexahydrate were purchased from Sigma-Aldrich (St. Louis, MO). Baby rabbit (3- to 4-week-old) and adult rabbit (8- to 12-week-old) complements were purchased from Pel-Freez Biologicals (Rogers, AR). Guinea pig and human complements were purchased from Rockland (Gilbertsville, PA) and Valley Biomedical (Winchester, VA), respectively. All complements were stored in aliquots at −80…
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