Gomes et al. (2014). Analytical Biochemistry. doi: 10.1016/j.ab.2013.10.018
…no. 3915) and Corning nonbinding surface (cat. no. 3991) were pur- chased from Corning (Corning, NY, USA). Heart and liver lysate preparation Livers and hearts from 3-month-old rats were obtained from Pel-Freez (Rogers, AR, USA). Tissues were minced with a razor blade and then immediately homogenized using a dounce homog- enizer with 2 ml buffer/0.1 g tissue of 26S buffer (50 mM Tris, 1 mM…