Complement Product Categories
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Popular Complement Serums
Applications
Vaccine Development
Well known and widely used in the vaccine industry, Pel-Freez enables a range of safety and efficacy assays including:
- Opsonophagocytic killing assays (OPKA)
- Serum bactericidal assays (SBA)
- Complement-dependent cytotoxicity assays (CDC)
With the ability to deliver bulk scales of consistently-performing human, rabbit and guinea pig complement, we can spare your teams the hassle of doing the preparation, maximizing efficiency, productivity, and consistency.
Frequently asked questions
Should I use human, rabbit, or guinea pig complement for my application?
Work backwards from the readout. Complement regulators are host-specific, so the complement source is part of the assay method, not a preference.
- Antibody-depleted human complement for the hSBA (serogroup B meningococcus and gonococcus; correlate hSBA ≥ 1:4). Neisseria recruits human factor H, which rabbit and guinea pig factor H cannot replace, and the donor antibodies in native human serum would kill the target on their own.
- Baby rabbit complement for the rSBA (serogroups A, C, W and Y; correlate rSBA ≥ 1:8) and for pneumococcal OPA/MOPA, which was standardized on it.
- Adult rabbit complement for complement-dependent cytotoxicity and HLA crossmatching, the classical cell-lysis source.
- Guinea pig complement where potency per volume is the point: CH50 titration, complement fixation and the vibriocidal assay.
When the model animal's own cascade is the readout, use matched-species complement. Mouse, rat, pig, canine, cynomolgus, goat and hamster complement are available on request through our custom quote form.
For what the research literature actually uses, by pathogen and assay, see which complement source for which assay.
Is complement activity affected by the antibody removal process?
The antibody removal process can result in decreased complement potency in hemolytic titer assays, however, we have optimized our process to limit the extent of this decrease. Each lot of complement is tested using the complement hemolytic titer assay at 50% dilution to confirm adequate functional activity.
How does freezing and thawing affect complement activity?
Multiple freeze-thaw cycles will result in reduced complement potency, so we offer a variety of pack sizes to satisfy applications ranging from small scale assay development experiments to large scale clinical studies. We recommend limiting the number of freeze-thaw cycles and sub-aliquot whenever possible in order to extend the shelf life of all of our complement products.
Research citing Complement Serums
8 peer-reviewed papers that used a product from this category, newest first.
All publications-
An mRNA–Lipid Nanoparticle Platform Encoding the Conserved Outer Membrane Protein BamA Elicits Broadly Cross-Reactive Systemic and Mucosal Antibodies Against Antimicrobial-Resistant Neisseria gonorrhoeae
Where the paper cites Pel-Freez
…362 Ng WHO X (1×10³ CFU in 40 µL HBSS) were added to wells containing diluted test sera 363 and incubated for 15 min at 37°C. Subsequently, 10 µL of either IgG/IgM-depleted normal human serum (NHS; Pel-Freeze Biologicals; Cat. # 34010-10, Lot 34943) or heat- inactivated NHS (HI-NHS; Cat. #34020-10, Lot 13844) was added as a 10% (v/v) complement source, and reactions were incubated for 1 h at 37°C. A single lot of…
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Do the protein-based meningococcal group B vaccines, 4CMenB and MenB-fHbp, elicit serum bactericidal antibody against meningococcal serogroup X clonal complex 181?
Where the paper cites Pel-Freez
…growth CHBA plate after a 4-h incubation. A volume of 10 µL of the bacterial suspension was added to each well of the microtiter plate. A volume of 10 µL of “active” antibody depleted human complement (Pel-Freeze, Inc., Brown Deer, WI) was added to all wells except the sample control wells for viable cfu and sample non-complement mediated lysis control, to which heat-inactivated antibody depleted human complement…
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Divergent humoral immune signatures in response to Klebsiella pneumoniae bacteremia
Where the paper cites Pel-Freez
…combined with 20 µl of heat-inactivated human serum (inactivated at 56°C for 30 minutes) in 96-well U-bottom plates and incubated shaking at 37°C for 1 h. Subsequently, 10 µl of baby rabbit complement (Pel-Freez Biologics) was added to a final concentration of 10%, and plates were incubated shaking for 1 h at 37°C. All samples were tested in triplicate. Controls included bacteria alone, bacteria with serum only,…
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Immunogenicity of primary and booster MenACWY-TT vaccination in older adults and the importance of IgM
Where the paper cites Pel-Freez
…Frailty Indicator self-report questionnaire35. Serological analysis Functional MenACWY-specific antibody titers were determined using the serum bactericidal assay with baby rabbit complement (rSBA) (Pelfreez, lot: 21044-C) as described previously15,36. Target strains included MenA (strain 3125), MenC (strain C11), MenW (strain MP01240070), and MenY (strain S-1975). rSBA titers were expressed as the reciprocal…
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Rational selection of TbpB variants yields a bivalent vaccine with broad coverage against Neisseria gonorrhoeae
Where the paper cites Pel-Freez
…allow opsonization, ~500 CFUs were incubated with various concentrations (1:20, 1:40, 1:80, 1:200, 1:400) of serum for 20 min, followed by the addition of 12% pooled human complement depleted of IgG/IgM (Pel Freez, cat. 34010, Lot 22344) and incubated for 60 min at 37 °C with 5% CO 2 . The highest dilution at which 50% killing was observed relative to no antibody control in 60 min was reported as the SBA titer.…
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Development and validation of a standardized human complement serum bactericidal activity assay to measure functional antibody responses to Neisseria gonorrhoeae
Where the paper cites Pel-Freez
…strain lacking pora expression and expressing a hybridized porb (och) [18 ]. see table s3 for a complete description of pc reagents. 3.4 intrinsic sba growth assay igg/igm-depleted human complement (c′) pooled serum (pel-freez validation lot #03642) was screened for endogenous killing activity against ng strains f62, ms11, and fa1090 as previously described [19 ] with modifications. briefly, sialylated or non-sial…
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