Human Complement, Pooled, IgG/IgM Depleted

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1. 2026Chanda et al. (2026). bioRxiv (Cold Spring Harbor Laboratory).

   An mRNA–Lipid Nanoparticle Platform Encoding the Conserved Outer Membrane Protein BamA Elicits Broadly Cross-Reactive Systemic and Mucosal Antibodies Against Antimicrobial-Resistant Neisseria gonorrhoeae

   Where the paper cites Pel-Freez

   …362 Ng WHO X (1×10³ CFU in 40 µL HBSS) were added to wells containing diluted test sera 363 and incubated for 15 min at 37°C. Subsequently, 10 µL of either IgG/IgM-depleted normal human serum (NHS; Pel-Freeze Biologicals; Cat. # 34010-10, Lot 34943) or heat- inactivated NHS (HI-NHS; Cat. #34020-10, Lot 13844) was added as a 10% (v/v) complement source, and reactions were incubated for 1 h at 37°C. A single lot of…

   • Human Serum, Pooled, Heat Inactivated, IgG/IgM Depleted

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2. 2026Ali et al. (2026). International Journal of Infectious Diseases.

   Do the protein-based meningococcal group B vaccines, 4CMenB and MenB-fHbp, elicit serum bactericidal antibody against meningococcal serogroup X clonal complex 181?

   Where the paper cites Pel-Freez

   …growth CHBA plate after a 4-h incubation. A volume of 10 µL of the bacterial suspension was added to each well of the microtiter plate. A volume of 10 µL of “active” antibody depleted human complement (Pel-Freeze, Inc., Brown Deer, WI) was added to all wells except the sample control wells for viable cfu and sample non-complement mediated lysis control, to which heat-inactivated antibody depleted human complement…

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3. 2025Fegan et al. (2025). npj Vaccines.

   Rational selection of TbpB variants yields a bivalent vaccine with broad coverage against Neisseria gonorrhoeae

   Where the paper cites Pel-Freez

   …allow opsonization, ~500 CFUs were incubated with various concentrations (1:20, 1:40, 1:80, 1:200, 1:400) of serum for 20 min, followed by the addition of 12% pooled human complement depleted of IgG/IgM (Pel Freez, cat. 34010, Lot 22344) and incubated for 60 min at 37 °C with 5% CO 2 . The highest dilution at which 50% killing was observed relative to no antibody control in 60 min was reported as the SBA titer.…

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4. 2024Reveille et al. (2024). Vaccine.

   Development and validation of a standardized human complement serum bactericidal activity assay to measure functional antibody responses to Neisseria gonorrhoeae

   Where the paper cites Pel-Freez

   …strain lacking pora expression and expressing a hybridized porb (och) [18 ]. see table s3 for a complete description of pc reagents. 3.4 intrinsic sba growth assay igg/igm-depleted human complement (c′) pooled serum (pel-freez validation lot #03642) was screened for endogenous killing activity against ng strains f62, ms11, and fa1090 as previously described [19 ] with modifications. briefly, sialylated or non-sial…

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• Salic acid protects nontypeable Haemophilus influenza from natural IgM and promotes survival in murine respiratory tract.

  Jackson, M. D., Wong, S. M. & Akerley, B. J. Sialic acid protects nontypeable haemophilus influenzae from natural igm and promotes survival in murine respiratory tract. Infect. Immun. 89, (2021).

• Development of Complement Factor H–Based Immunotherapeutic Molecules in Tobacco Plants Against Multidrug-Resistant Neisseria gonorrhoeae

  Shaughnessy, J. et al. Development of Complement Factor H–Based Immunotherapeutic Molecules in Tobacco Plants Against Multidrug-Resistant Neisseria gonorrhoeae. Front. Immunol. 11, 2680 (2020).

• Synthetic DNA Delivery of an Optimized and Engineered Monoclonal Antibody Provides Rapid and Prolonged Protection against Experimental Gonococcal Infection

  Parzych, E. M. et al. Synthetic dna delivery of an optimized and engineered monoclonal antibody provides rapid and prolonged protection against experimental gonococcal infection. MBio 12, 1–13 (2021).

• Neisseria meningitidis urethritis outbreak isolates express a novel factor H binding protein variant that is a potential target of group B-directed meningococcal (MenB) …

  Tzeng, Y. L. et al. Neisseria meningitidis urethritis outbreak isolates express a novel factor H binding protein variant that is a potential target of group B-Directed meningococcal (menb) vaccines. Infect. Immun. 88, (2020).

• Vaccination with Detoxified Leukocidin AB Reduces Bacterial Load in a Staphylococcus aureus Minipig Deep Surgical Wound Infection Model

  Fernandez, J. et al. Vaccination with Detoxified Leukocidin AB Reduces Bacterial Load in a Staphylococcus aureus Minipig Deep Surgical Wound Infection Model . J. Infect. Dis. (2021).

• Preclinical efficacy of a lipooligosaccharide peptide mimic candidate gonococcal vaccine

  Gulati, S. et al. Preclinical efficacy of a lipooligosaccharide peptide mimic candidate Gonococcal vaccine. MBio 10, (2019).

• Complement-Dependent Serum Bactericidal Assays for Neisseria gonorrhoeae

  Gulati, S., Rice, P. A. & Ram, S. Complement-Dependent Serum Bactericidal Assays for Neisseria gonorrhoeae. in Methods in Molecular Biology vol. 1997 267–280 (Humana Press Inc., 2019).

• Development of Complement Factor H–Based Immunotherapeutic Molecules in Tobacco Plants Against Multidrug-Resistant Neisseria

  Shaughnessy, J. et al. Development of Complement Factor H–Based Immunotherapeutic Molecules in Tobacco Plants Against Multidrug-Resistant Neisseria gonorrhoeae. Front. Immunol. 11, (2020).

Human Complement Serum