Publications

272 publications

  1. 2016Singh et al. (2016). Investigative Ophthalmology & Visual Science.

    Evaluating the Effects of Riboflavin/UV-A and Rose-Bengal/Green Light Cross-Linking of the Rabbit Cornea by Noncontact Optical Coherence Elastography

    Where the paper cites Pel-Freez

    …of the cross-linking treatments were revealed by depth- resolved micrometer-scale phase velocity distribution.50 METHODS Cornea Preparation Mature rabbit eyes (>6 months old) were obtained fresh (Pel- Freeze Biologicals, Rogers, AR, USA) and shipped overnight on ice. Samples were separated into two groups for the cross- linking experiments (n ¼ 4 samples for each group). Extraneous tissues such as muscles were…

  2. 2016Gopinath et al. (2016). Forensic Science International Genetics.

    Developmental validation of the Yfiler® Plus PCR Amplification Kit: An enhanced Y-STR multiplex for casework and database applications

    Where the paper cites Pel-Freez

    …male component). Performance measured as allele recovery was benchmarked against that of the Yfiler Kit. 2.9 Species specificity Whole blood samples for the following species were obtained from Pel- Freez Biologicals (Rogers, AK USA): Bovine, Chicken, Mouse, Pig, Rabbit, Dog, Rat, Horse, and Sheep. Genomic DNA from these whole blood samples was extracted and purified using the ABI® PRISM 6100 Nucleic Acid…

  3. 2016Tao-Cheng et al. (2016). PLOS ONE.

    Zinc stabilizes Shank3 at the postsynaptic density of hippocampal synapses

  4. 2016Yun et al. (2016). Microbial Pathogenesis.

    Serum bactericidal assay for the evaluation of typhoid vaccine using a semi-automated colony-counting method

    Where the paper cites Pel-Freez

    …4-nitrophenyl phosphate disodium hexahydrate were purchased from Sigma-Aldrich (St. Louis, MO). Baby rabbit (3- to 4-week-old) and adult rabbit (8- to 12-week-old) complements were purchased from Pel-Freez Biologicals (Rogers, AR). Guinea pig and human complements were purchased from Rockland (Gilbertsville, PA) and Valley Biomedical (Winchester, VA), respectively. All complements were stored in aliquots at −80…

  5. 2016Cui et al. (2016). Scientific Reports.

    Quantification of dopaminergic neuron differentiation and neurotoxicity via a genetic reporter

    Where the paper cites Pel-Freez

    …t 1:200, TH (Pel Freez) at 1:500, TUJ (Abcam) at 1:1000. Directed dopaminergic differentiation and quantification of TH using luciferase assay. ES cells were differentiated towards a dopamingeric neuron cell fate for up to 6 weeks following the floor-plate induction method23. Cell supernatants were collected every day for the first 20 days of neuronal differentiation and every other day afterwa

  6. 2016Narasimhan et al. (2016). Journal of Biomedical Nanotechnology.

    Polyanhydride nanovaccines induce germinal center B cell formation and sustained serum antibody responses

    Where the paper cites Pel-Freez

    …stained with combina- tions of fluorochrome-conjugated antibodies to identify various cell subsets. Non-specific binding of conjugated antibodies was inhibited by blocking cells with 10 L of rat serum (Pel Freez, Rogers, AR) and 10 g of 2.4G2, an anti-FcR monoclonal antibody. Rat anti-mouse mon- oclonal antibodies used were anti-IgM (b7-6), anti-B220 (6B2), anti-CD44 (9F3), anti-CD4 (PerCP/Cy5.5 conju- gate,…

  7. 2016Hoteit et al. (2016). Gene Reports.

    KIR genotype distribution among patients with chronic lymphocytic leukemia: Is there a role for KIR 2DS4 and KIR 2DS5 genes?

    Where the paper cites Pel-Freez

    …om donors of a bone marrow transplanta- tion center representing all ethnicities and districts in the country. DNA extraction and KIR genotyping PEL-FREEZ kits (Pel-freez/Dynal, Norway) were used for DNA ex- traction from 2 to 3 ml of collected peripheral blood. The DNA material was properly labeled and stored at −80 °C. Based on our Institutional Review Board (IRB) committee rules and study ap

  8. 2016Walter et al. (2016). The Journal of Comparative Neurology.

    Individual sympathetic postganglionic neurons coinnervate myenteric ganglia and smooth muscle layers in the gastrointestinal tract of the rat

    Where the paper cites Pel-Freez

    …TH purified from rat PC12 cells, and the antibody is described as having wide species cross- reactivity (manufacturer’s datasheet). The immunogen used to produce the rabbit polyclonal TH antibody (Pel- Freez Biologicals, Cat. no. P40101, RRID:AB_2313713) was SDS-denatured TH from rat pheochromocytoma, and shown by the manufacturer using western blot analysis to be specific for the 60 kD TH protein in rat caudate…

  9. 2016Rebbeck et al. (2016). SLAS DISCOVERY.

    High-Throughput Screens to Discover Small-Molecule Modulators of Ryanodine Receptor Calcium Release Channels

    Where the paper cites Pel-Freez

    …mpound NCC was purchased from Evotec (Hamburg, Germany) as 10 mM solutions in DMSO. The pig skeletal muscle (longissimus dorsi) was processed and purchased from Pel-Freez Biologicals (Rogers, AR). Fluorophores Alexa Fluor 488 C5-maleimide (AF488) and Alexa Fluor 568 C5-maleimide (AF568) were purchased from Life Technologies (Eugene, OR). Individual NCC compounds of interest (hits) were purchase

  10. 2016Dunya et al. (2016). European Archives of Oto-Rhino-Laryngology.

    Head and neck cutaneous leishmania: clinical characteristics, microscopic features and molecular analysis in a cohort of 168 cases

    Where the paper cites Pel-Freez

    …o our previously reported protocol. In brief, for FFPE tissue blocks, DNA was extracted from ribbons originating from FFPE. For scraping, the DNA extraction was done using the Pel-Freez (Invitrogen, Carlsbad, CA, USA) kit according to the standard manufacturer’s instructions. The DNA samples were incubated at 65 C for 30 min and then stored at 4 C until analyzed by PCR. The resultant DNA was

  11. 2016Nahm et al. (2016). ASM Press eBooks.

    Functional Assays for B Cells and Antibodies

    Where the paper cites Pel-Freez

    …HL-­60 cell maintenance) (no. SH30080.03; HyClone) Premium fetal bovine serum (FBS, for preparation of assay buffer [OBB]) (no. 11150; Atlanta Biologicals) Baby rabbit complement (BRC) (no. 31061; Pel-­Freez Biologicals) HL-­60 cell line (no. CCL240; ATCC) Blood agar plates Glycerol Gelatin Dimethyl sulfoxide (DMSO) Target bacteria (see Table 4) Prepared Solutions Streptomycin stock solution Prepare a 300 mg/ml…

  12. 2016Kim et al. (2016). Transfusion.

    Functional antibodies to Haemophilus influenzae type B, Neisseria meningitidis, and Streptococcus pneumoniae contained in intravenous immunoglobulin products

    Where the paper cites Pel-Freez

    …salt solution (HBSS) with Ca21 and Mg21 (Life Technologies) and 0.1% gelatin and aliquoted in a 96-well microplate. Diluted Hib Eagan strain suspension (10 mL) and 10 mL of baby rabbit complement (Pel-Freeze Biologicals) were added in the 96-well microplate. The mixture was incu- bated for 2 minutes at 258C with shaking at 700 rpm and then incubated for 30 min at 378C/5% CO2 without shak- ing. After stopping the…

  13. 2016Ramachandran et al. (2016). PLOS ONE.

    Functional Activity of Antibodies Directed towards Flagellin Proteins of Non-Typhoidal Salmonella

  14. 2016Kakuki et al. (2016). Oncotarget.

    Dysregulation of junctional adhesion molecule-A via p63/GATA-3 in head and neck squamous cell carcinoma

  15. 2016Fadlallah et al. (2016). Investigative Ophthalmology & Visual Science.

    Corneal Resistance to Keratolysis After Collagen Crosslinking With Rose Bengal and Green Light

    Where the paper cites Pel-Freez

    …mons Attribution-NonCommercial-NoDerivatives 4.0 International License. Downloaded From: https://iovs.arvojournals.org/ on 08/19/2018 covered with aluminum foil to protect them from light and stored at 48C until use. Tissue Preparation Frozen mature New Zealand White rabbit eyes were obtained frozen (Pel-Freez Biologicals, Rogers, AR, USA) and thawed in 13PBS at room temperature for 1 hour befo

  16. 2016McCorry et al. (2016). Stem Cell Research & Therapy.

    Characterization of mesenchymal stem cells and fibrochondrocytes in three-dimensional co-culture: analysis of cell shape, matrix production, and mechanical performance

    Where the paper cites Pel-Freez

    …0:100, 25:75, 50:50, 75:25, and 100:0. Since no live animals were used in this study, no IACUC approval was required. Construct generation Collagen type I was extracted from Sprague–Dawley rat tails (Pel-Freez Biologicals, Rogers, AZ, USA) and recon- stituted in 0.1 % acetic acid at 30 mg/mL concentration as previously described [25, 29, 30]. Briefly, the stock collagen solution was mixed with

  17. 2016Nuriel et al. (2016). Frontiers in Chemistry.

    ANSID: A solid-phase proteomic approach for identification and relative quantification of aromatic nitration sites

  18. 2016Hayakawa et al. (2016). Journal of Biological Chemistry.

    Actin-interacting Protein 1 Promotes Disassembly of Actin-depolymerizing Factor/Cofilin-bound Actin Filaments in a pH-dependent Manner

    Where the paper cites Pel-Freez

    …Thus, AIP1 may function as a novel pH sensor for regulation of actin filament dynamics. EXPERIMENTAL PROCEDURES Proteins and Materials- Rabbit muscle actin was purified from acetone powder (Pel- Freeze Biologicals) as described previously (53). UNC-60B was expressed in Escherichia coli and purified as described (48). GST alone was expressed from pGEX-2T (GE Healthcare Life Sciences) and purified using…

  19. 2016Kim et al. (2016). BMC Infectious Diseases.

    A high throughput serum bactericidal assay for antibodies to Haemophilus influenzae type b

    Where the paper cites Pel-Freez

    …Ca2+ and Mg2+ [Life Technologies, Grand Island, NY, USA] and 0.1 % gelatin). A frozen aliquot of Hib Eagan strain [14] was diluted in the dilution buffer to yield 750 colony forming unit (CFU) in 10-μL. Twenty μl of diluted serum was mixed with 10 μL of Hib solution and 10 μL of baby rabbit complement (Pel-Freez Biologicals, Brown Deer, WI, USA) in a microwell. The mixture was incubated for 2 m

  20. 2016Abraham et al. (2016). Arthritis & Rheumatology.

    Brief Report: Anti-Eukaryotic Initiation Factor 2B Autoantibodies Are Associated With Interstitial Lung Disease in Patients With Systemic Sclerosis

    Where the paper cites Pel-Freez

    …were screened at 1:40 and where anti- eIF2B positive, titred by serial dilution. Counter Immune Electrophoresis CIEP was performed as described previously (7) using lyophilised rabbit thymus extract (Pel- Freez, USA) and bovine spleen extract in PBS (Sigma). Protein Immunoprecipitation (IPP) using [35S]-methionine 10 µl sera was mixed with 2 mg protein-A-Sepharose beads (Sigma, UK) in IPP buffer (10 mM Tris-Cl…

  21. 2016Han et al. (2016). Laser Physics Letters.

    Analysis of the effect of the fluid-structure interface on elastic wave velocity in cornea-like structures by OCE and FEM

  22. 2016Konstantinov et al. (2016). Biosensors and Bioelectronics.

    Biosensor for total antinuclear antibody determination at the point-of-care

    Where the paper cites Pel-Freez

    …assay, Pierce Biotechnology), and was stored in 1.0 ml aliquots at 70 °C. Chromatin (chr), which is the natural DNA-histone macromolecular complex present in the nucleus, was purified from calf thymus (Pel-Freez) and stripped of histone H1 as previously described (Burlingame and Rubin, 1990) to maintain solubility. The concentration of its DNA component was de- termined by absorbance at 260 mμ based on E¼25 for…

  23. 2015van Amsterdam et al. (2015). Annals of Anatomy - Anatomischer Anzeiger.

    The morphological substrate for Renal Denervation: Nerve distribution patterns and parasympathetic nerves. A post-mortem histological study

    Where the paper cites Pel-Freez

    …using a 3% H2O2 solution in TBS with 0.1% Triton X-100 (Tx). Thereafter, different protocols were used for various neural markers. Polyclonal antibodies to Tyrosine Hydroxylase (TH, P40101-0, Pel- freez biologicals, Rogers, AR USA), Nitric Oxide Synthase (NOS, AB15203, Abcam, Cambridge UK) and Calcitonin Gene Related Peptide (CGRP, PA1-18039, Pierce Biotechnology, Rockford, IL USA) were used as markers for…

  24. 2015Soylu-Kucharz et al. (2015). Scientific Reports.

    Hypothalamic overexpression of mutant huntingtin causes dysregulation of brown adipose tissue

    Where the paper cites Pel-Freez

    …Next, the sections were left overnight at RT in primary antibody solu- tion in TBS-T containing 5% normal goat serum. The primary antibodies dilutions were: anti-tyrosine hydroxylase (1:2000; rabbit; Pel-Freez), anti-huntingtin (sc-8767; 1:500; goat; Santa Cruz), anti-ubiquitin (1:2000; rabbit; Dako), anti-GFP (ab290; 1:30000; rabbit; Abcam), anti-orexin (1:4000; rabbit; Phoenix Pharmaceuticals). Following the…

  25. 2015Pezzanite et al. (2015). Stem Cell Research & Therapy.

    Equine allogeneic bone marrow-derived mesenchymal stromal cells elicit antibody responses in vivo

    Where the paper cites Pel-Freez

    …suspen- sion were incubated for 30 minutes at room temperature under oil in wells of Terasaki plates (Robbins Scientific Corporation, Sunnyvale, CA, USA), following which 5 μl of rabbit complement (Pel-Freez Biologicals, Rogers, AR, USA) was added for an additional 1 hour. The wells were then stained with 2 μl of 5% eosin dye and fixed with 5 μl of 37% formalin (pH between 7.2 and 7.4). The cytotoxic antibody…

  26. 2015Zavalin et al. (2015). Analytical and Bioanalytical Chemistry.

    Tissue protein imaging at 1 μm laser spot diameter for high spatial resolution and high imaging speed using transmission geometry MALDI TOF MS

    Where the paper cites Pel-Freez

    …de (ITO)-coated microscope glass slides were purchased from Delta Technologies (Stillwater, MN, USA). Carnoy’s fluid was prepared as a 6:3:1 solution of 100 % ethanol/chloroform/acetic acid. Fresh frozen mouse brain was purchased from Pel-Freez Biologicals (Rogers, AZ, USA). Sample preparation Tissue preparation, rinsing steps, and matrix spraying proto- cols were optimized to maximize ionizati

  27. 2015Lin, H. et al. Three-Dimensional Culture of Functional Adult Rabbit Lacrimal Gland Epithelial Cells on Decellularized Scaffold. Tissue Eng. - Part A 22, 65-74 (2016).

    Three-dimensional culture of functional adult rabbit lacrimal gland epithelial cells on decellularized scaffold

    Where the paper cites Pel-Freez

    …a 26 gauge syringe, and passed over a 40 μm mesh nylon cell strainer. Tissue decellularization To avoid sacrificing live animals for scaffold preparation, NZW rabbit lacrimal glands, purchased from Pel-Freez Biologicals (Rogers, AR) were decellularized based on our lab’s protocols. Briefly, NZW rabbit lacrimal glands excised from orbits were treated with 1% sodium dodecyl sulfate (SDS) or 1% Triton X-100 for 36…

  28. 2015Ahn et al. (2015). Vaccine.

    Functional immune responses to twelve serotypes after immunization with a 23-valent pneumococcal polysaccharide vaccine in older adults

    Where the paper cites Pel-Freez

    …CFU in each well of a 96-well microtiter plate. After 30 min of incubation at room temperature with shaking, 40 L of HL-60 cell suspension (4 × 105 cells per well) and 10 L of baby rabbit complement (Pel-Freez, Brown Deer, WI, USA) were added to each well. Plates were incubated in a tissue culture incubator (37 ◦C, 5% CO2) with shaking for 45 min. An aliquot of the final reac- tion mixture (10 L) was spotted…

  29. 2015Latta et al. (2015). Journal of Neuroinflammation.

    Determining the role of IL-4 induced neuroinflammation in microglial activity and amyloid-β using BV2 microglial cells and APP/PS1 transgenic mice

    Where the paper cites Pel-Freez

    …DPBS, transferred to a clean 12-well plate, fixed in 10% neutral buffered formalin (Sigma-Aldrich, St Louis, MO) for 20 min and rinsed twice in 1× DPBS for 5 min. Cells were blocked in 0.019% L-lysine, 0.3% triton-X (Sigma-Aldrich, St Louis, MO) and 4% goat serum (Pel-Freez, Rogers, AR) in DPBS for 45 min and incubated in primary antibody CD11b 1:500 (Rat monoclonal, AbD Serotec, Raleigh, NC) i

  30. 2015Ogunjirin et al. (2015). Neurochemical Research.

    Competition, Selectivity and Efficacy of Analogs of A-84543 for Nicotinic Acetylcholine Receptors with Repositioning of Pyridine Nitrogen

    Where the paper cites Pel-Freez

    …cy compounds may have some advantages. Materials Unless otherwise noted, all chemicals were purchased from Sigma Aldrich (St. Louis, Mo). [3H]EB was purchased from Perkin Elmer Life and Analytical Sciences (Boston, MA). Adult male rat whole brains were purchased from Pel-Freez Biologicals (Rogers, Arkansas). Methods For these studies we designed, synthesized and character- ized a set of new nAC