Publications
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TWIK-1 plays distinct roles in spinal and peripheral sensory circuits controlling mechanical sensitivity and neuropathic hypersensitivity
Where the paper cites Pel-Freez
…No. 019-19741), goat anti-CGRP (1:1000, Abcam, Cat. No. ab36001), rabbit anti-CSF1R (1:100, Abcam, Cat. No. ab183316), chicken anti-NF200 (1:500, Aves labs, Cat. No. AB_2313552), rabbit anti-TH (1:1000, Pel-Freez, Cat. No. P40101- 150), and rabbit anti-mCherry (1:1000, Abcam, Cat. No. ab167453). The secondary antibodies used were Alexa Fluor-conjugated donkey anti-rabbit (1:500, Life Technologies, Cat. No.…
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Loss of PINK1 causes age-dependent mitochondrial trafficking deficits in nigrostriatal dopaminergic neurons via aberrant p38 MAPK activation
Where the paper cites Pel-Freez
…an investigator blinded to the genotype. ARTICLE IN PRESS ARTICLE IN PRESS Antibodies and Reagents The antibodies and reagents used in the study include: Anti-Tyrosine Hydroxylase (rabbit, 1:5000 for IF, Pel-Freez, P40101-0), Anti-Tyrosine Hydroxylase (mouse, 1:500 for IF, Millipore, MAB5280), Anti-Kinesin 5B heavy chain (mouse, 1:500 for WB, Millipore, MAB1614), Anti- Dynein medium chain (mouse, 1:1000 for WB,…
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High molecular weight insoluble parkin in the substantia nigra of patients with idiopathic Parkinson’s disease
Where the paper cites Pel-Freez
…1 h at ambient temperature. As for the immunoblots, the antibodies against parkin C-Terminal region (monoclonal parkin clone [PRK8], 1:1,000 and polyclonal parkin (cell signaling cat#2132, 1:1,000)), TH (Pel-Freez Biolo- gicalsCat# P40101-150, 1:1,000),αsyn(SYN1,BDBiosciencesCat# 610787, 1:1,000), MJFR1 (Abcam Cat# ab138501 [MJFR1], 1:1,000) and αsyn phosphorylatedatserine129(αsynP129)(AbcamCat#ab168381[MJF-R13…
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Targeting reverse electron transport preserves mitochondrial function and prevents dopaminergic neurodegeneration in Parkinson’s disease
Where the paper cites Pel-Freez
…then incubated at 4 °C overnight with a rabbit polyclonal primary antibody against TH, GFAP or IBA-1 in KPBS containing 0.4% triton X-100 (TH, Pel Freez #P40101- 150, 1:1000; GFAP, Proteintech #16,825–1-AP 1:500; and Iba-1, Novus biologicals Cat#NBP2-19,019, 1:500). After five to six rinses in KPBS, the sections were treated for two hours in a biotinylated secondary antibody (PK- 6200, 1:1000,
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Miro1 mutations disrupt cellular calcium homeostasis via dysregulation of mitochondria-ER-contact-sites, rendering iPSC-derived neurons more susceptible to lipid peroxidation
Where the paper cites Pel-Freez
…Peprotech, 450-10-50UG). Neuronal cultures were assessed by immunostaining with antibodies against Mikrotubuli-associated protein 2 (MAP2; Abcam ab5392, dilution 1:10000) and Tyrosin Hydroxylase (TH; Pel-Freez P40101, dilution 1:1000). 2.2 Live cell imaging of mitochondrial calcium Neurons were cultivated in 8 well chamber slides (Ibidi, 80806) at 100,000 cells per well. Then, neurons were transfected with 0.5…
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Integration of cross-species multi-omics with in vivo experimental validation identifies Parkinson's disease therapeutic targets and novel risk factors within endolysosomal pathway subnetworks
Where the paper cites Pel-Freez
…times for 5 min each using 1× PBST. Samples were placed in blocking buffer (1% bovine serum albumen in PBST) for 1 h at room temperature and then incubated with anti-Tyrosine Hydroxylase (1:500, P40101 PelFreez) for 3 days at 4 °C. After the primary was removed, samples were washed 3 times with 1× PBST for 5 min each, then incubated in secondary (1:500 Alexa Fluor anti-Rabbit 555 (A-21428) and 1:1000 DAPI) for 1…
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A novel rat model of body first subtype of Parkinsons disease
Where the paper cites Pel-Freez
…for 1 hour at room temperature to block nonspecific binding sites and then incubated in primary antibody (rabbit anti- tyrosine hydroxylase, Pel Freez Biologicals #P40101-150, 1:250 in blocking solution) for 72 hours at 4oC on a shaker. Sections were washed in between incubation steps with 6 x 3min PBS-Tx. Sections were incubated in diluted secondary antibody (biotinylated donkey anti-rabbit Ig
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Signal Transduction Pathway Mediating Carotid Body Dependent Sympathetic Activation and Hypertension by Chronic Intermittent Hypoxia
Where the paper cites Pel-Freez
…CM1900), and mounted on collagen-coated coverslips. Sections were blocked in PBS containing 1% normal goat serum and 0.2% Triton X-100 and then incubated with monoclonal rabbit anti-TH antibody (Pel-Freez Biologicals; #P40101-0; dilution 1:4000), followed by 5 washes with PBS containing 0.05% Triton X-100. Antibody binding was detected using Texas red-conjugated goat anti-mouse IgG diluted 1:250 in PBS…
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RNAi-based screen for pigmentation in Drosophila melanogaster reveals regulators of brain dopamine and sleep
Where the paper cites Pel-Freez
…the critical analysis of the article. Declaration of interests The authors declare no competing interests. STAR★Methods Key resources table REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-TH Pel-Freez Biologicals P40101, RRID:AB_461064 anti-elav DSHB 7E8A10, RRID:AB_528218 anti-rat-A488 Thermo Fisher Sci A-21208, RRID:AB_2535794 anti-rabbit-A647 Thermo Fisher Sci A-27040, RRID:AB_2536101 Chemicals, peptides,…
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Protocol for differentiating human pluripotent stem cells into midbrain organoids for targeted microinjection of viruses
Where the paper cites Pel-Freez
…74376 NESTIN (dilution 1:1,000) Santa Cruz Cat#sc-21247 OTX2(dilution 1:1,000) R&D Systems Cat# AF2018 DCX (dilution 1:1,000) CST Cat#4604 SOX2 (dilution 1:500) R&D Systems Cat#AF2018 TH (dilution 1:1,000) Pel-Freez Cat#P40101 GAD67 (dilution 1:1,000) Millipore Cat#MAB5406 Donkey anti-mouse Alexa Fluor 488 (dilution 1:1,000) Thermo Fisher Scientific Cat#A21202 Donkey anti-rabbit Alexa Fluor 488 (dilution 1:1,000)…
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Neurotherapeutic effects of Vutiglabridin as a Paraoxonase-2 modulator in preclinical models of Parkinson’s disease
Where the paper cites Pel-Freez
…d dilution and the sec tions were incubated in a shaker at 4 °C overnight. Pri mary antibodies used for immunostaining were anti-TH (rabbit, 1:200, Pel-freez, P40101-150), GFAP (chicken, 1:500, Millipore, AB5541), Iba-1 (rabbit, 1:500, Wako, 019-19741), DDC (rabbit, 1:200, abCam, ab3905), GABA (guinea pig, 1:200, Millipore, AB175), anti-PON2 (rab bit, 1:200, abCam, ab183710). Unbound antibod
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Molecularly distinct striatonigral neuron subtypes differentially regulate locomotion
Where the paper cites Pel-Freez
…Antifade Mountant, Life technology). Images were taken using a laser scanning confocal microscope LSM 780 (Zeiss). The primary antibodies used for immunostaining included rabbit monoclonal anti-TH (Pel-Freez Biologicals, P40101; dilution 1:1000), mouse monoclonal anti-TH (ImmunoStar, 22941; dilution 1:1000), chicken polyclonal anti-TH (Aves Labs, TYH; dilution 1:500), rabbit anti-Mu-opioid receptor (MOR)…
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Microglia depletion reduces neurodegeneration and remodels extracellular matrix in a mouse Parkinson’s disease model triggered by α-synuclein overexpression
Where the paper cites Pel-Freez
…30 min to eliminate endogenous oxidase. The samples were then blocked with 5% normal goat serum (ZSGB-Bio, Cat#: ZLI-9021, Lot#: 190320) for 1 h at room temperature, then incubated with rabbit anti-TH (Pel-Freez, Cat#: P40101, Lot#: aj0520P, 1:1000) or rabbit anti-NeuN (EMD Millipore Corp, Cat#: ABN78, Lot#: 3692639, 1:500) overnight. On the second day, the brain slices were incubated with a secondary antibody…
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Impulsive-compulsive behaviours and striatal neuroactivity in mildly parkinsonian rats under D2/3 agonist and L-DOPA treatment
Where the paper cites Pel-Freez
…analysis. For quantifying the extent of striatal dopaminergic denervation and counting of pS6 + cells, bright-field immunohistochemistry was performed using a primary antibody against TH (rabbit anti-TH, Pel-Freez P40101, 1:1000) and phosphorylated pS6, respectively (monoclonal rabbit anti Ser235/236-phospho-S6, Cell Signaling #2211, 1:200). Immunocomplexes were revealed using biotinylated secondary antibodies…
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Hemoglobin as a pseudoperoxidase and drug target for oxidative stress-related diseases
Where the paper cites Pel-Freez
…anti-Hbβ (1:200, Abcam, ab214049), mouse anti-Hbβ (1:200, Santacruz, sc-21757), mouse anti-MAOB (1:200, Santacruz, sc-515354), mouse anti-8-OhdG (1:500, Santacruz, sc-393871), rabbit anti-TH (1:500, Pelfreez, p40101), and mouse anti-TH (1:200, Sigma, T2928). The following secondary antibodies from Jackson were diluted (1:500) in blocking solution: donkey-anti-chicken 405, donkey-anti-chicken 647,…
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Activation of the Carotid Body by Kappa Opioid Receptors Mitigates Fentanyl-Induced Respiratory Depression
Where the paper cites Pel-Freez
…ry neurons by µ opioid receptors and suggest that KOR agonists might counteract the central depressive effects of opioids on breathing. … The primary antibodies used were rabbit anti-TH antibody (Pel-Freez Biologicals; #P40101-0; dilution 1:1000), mouse anti-TH antibody (Sigma, #T1299, dilution 1:1000), guinea pig anti…
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The therapeutic use of clonal neural stem cells in experimental Parkinson´s disease
Where the paper cites Pel-Freez
…owing primary anti bodies were used: TH (1:400; Pel-Freez P40101-150), glial fibrillary acidic protein (GFAP) (1:1000; DAKO Z0334), ionized calcium-binding adapter molecule 1 (Iba1) (1:1000; Wako 019-19741), nestin (NES) (1:300; Novus Biologicals NB100-1604), Ki-67 (1:100; Ther moFisher Scientific RM-9106-S1), doublecortin (DCX) (1:300; Santa Cruz sc-8066), STEM121 (1:500; Takara Bio Y40410),
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Catecholaminergic axon innervation and morphology in flat‐mounts of atria and ventricles of mice
Where the paper cites Pel-Freez
…ript Author Manuscript Author Manuscript Author Manuscript Author Manuscript Author Manuscript Author Manuscript Author Manuscript Bizanti et al. Page 43 Table 1: Antibodies used in immunohistochemistry: Antibody Concentration Host Company Catalog number Excitation RRID Anti-TH # 12 μL/mL Rabbit Pel-Freeze P40101 n/a AB_461064 Anti-Rabbit ## 24 μL/mL Donkey Invitrogen A-21207 594 nm AB_2762827
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RGS14 modulates locomotor behavior and ERK signaling induced by environmental novelty and cocaine within discrete limbic structures
Where the paper cites Pel-Freez
…buffer, including primary antibodies raised against pERK (rabbit an- ti-Phospho-p44/42 MAPK, Cell Signaling Technology, Danvers, MA, #4370; 1:1000) or TH (rabbit anti-TH, P40101-0, Pel-Freez, Rogers, AR; 1:1000) or STEP (rabbit anti-Non-phospho-STEP, Cell Signaling Technology, #5659; 1:500) and RGS14 (mouse anti-RGS14, NeuroMab, Davis, CA, clone N133/21; 1:500). The sections were then…
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Upregulating β-hexosaminidase activity in rodents prevents α-synuclein lipid associations and protects dopaminergic neurons from α-synuclein-mediated neurotoxicity
Where the paper cites Pel-Freez
…Triton-X, blocked in 10% normal goat serum in PBS 0.3% Triton-X, and primary antibodies were applied overnight at 4C. Primary antibodies used were rabbit anti-tyrosine hydroxylase (Pel-Freez Biologi- cals Cat# P40101, RRID:AB_2313713), mouse anti-aSYN (human) (Millipore Cat# 36–008, RRID:AB_310817), mouse anti-aSYN (pan species) (BD Biosciences Cat# 610786, RRID:AB_398107), mouse anti-LAMP-1 (S
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Slc6a3-dependent expression of a CAPS-associated Nlrp3 allele results in progressive behavioral abnormalities and neuroinflammation in aging mice
Where the paper cites Pel-Freez
…(AL177, AdipoGen Life Science, San Diego, CA), interleukin-1 beta (IL-1β) (AF-401-NA, R&D Systems, Minneapolis, MN), and tyrosine hydroxylase (TH) (P40101, Pel-Freez Biologicals, Rogers, AR). IHC studies were conducted with either BOND polymer refined de- tection system (Leica Biosystems, Wetzlar, Germany) or HRP Polymer (Biocare Medical, Pacheco, CA). Images were obtained using an Olympus BX51
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An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions
Where the paper cites Pel-Freez
…Cruz Biotechnology Sc-990, RRID:AB_2298676 Goat polyclonal anti-OTX2 R&D Systems AF1979, RRID:AB_2157172 Sheep polyclonal anti-PITX2 R&D Systems AF7388, RRID:AB_11128639 Rabbit polyclonal anti-TH Pel-Freez Biologicals P40101-0, RRID:AB_461064 Sheep polyclonal anti-TH Pel-Freez Biologicals P60101-0, RRID:AB_461070 Chemicals, Peptides, and Recombinant Proteins Accutase Innovative Cell Technologies AT104 Ascorbic…
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Neuroprotection and immunomodulation of progesterone in the gut of a mouse model of Parkinson's disease
Where the paper cites Pel-Freez
…blue solution to colour myenteric ganglia neu‐ rones.47 Dopaminergic neurones were detected by an overnight incu‐ bation with a polyclonal primary antibody for TH (rabbit anti‐tyrosine hydroxylase; Pel Freez, Rogers, AR, USA; catalogue no. P40101‐150; dilution 1:1000) followed by incubation with a biotinylated goat anti‐ rabbit secondary antibody (Vector Laboratories, Inc., Burlingame, CA, USA; catalogue no.…
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Individual sympathetic postganglionic neurons coinnervate myenteric ganglia and smooth muscle layers in the gastrointestinal tract of the rat
Where the paper cites Pel-Freez
…TH purified from rat PC12 cells, and the antibody is described as having wide species cross- reactivity (manufacturer’s datasheet). The immunogen used to produce the rabbit polyclonal TH antibody (Pel- Freez Biologicals, Cat. no. P40101, RRID:AB_2313713) was SDS-denatured TH from rat pheochromocytoma, and shown by the manufacturer using western blot analysis to be specific for the 60 kD TH protein in rat caudate…
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The morphological substrate for Renal Denervation: Nerve distribution patterns and parasympathetic nerves. A post-mortem histological study
Where the paper cites Pel-Freez
…using a 3% H2O2 solution in TBS with 0.1% Triton X-100 (Tx). Thereafter, different protocols were used for various neural markers. Polyclonal antibodies to Tyrosine Hydroxylase (TH, P40101-0, Pel- freez biologicals, Rogers, AR USA), Nitric Oxide Synthase (NOS, AB15203, Abcam, Cambridge UK) and Calcitonin Gene Related Peptide (CGRP, PA1-18039, Pierce Biotechnology, Rockford, IL USA) were used as markers for…
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Brown Adipose Tissue Transplantation Reverses Obesity in Ob/Ob Mice
Where the paper cites Pel-Freez
…immunostaining procedure was used to the detection of tyrosine hydroxylase (TH). Tissues specimens were blocked with 10% normal goat serum for 30 minutes and then incubated with TH antibody (Pel FreeZ, P40101–150) overnight at 4°C, followed by a 1 hour incubation at room temperature with HRP-conjugated goat an- tirabbit IgG. To quantify the size of adipocyte, more than five sections were taken from…
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MicroRNA-7 Protects against 1-Methyl-4-Phenylpyridinium-Induced Cell Death by Targeting RelA
Where the paper cites Pel-Freez
…RelA J. Neurosci., September 17, 2014 • 34(38):12725–12737 • 12727 Related Disorders at the Banner Sun Health Research Institute. Incuba- tion with rabbit anti-TH antibody (1:500; catalog #P40101-0, Pel-Freez Biologicals) for IF labeling was performed along with anti-digoxigenin- POD antibody. Tissue sections were incubated with anti-rabbit-TRITC secondary antibody (1:1000; catalog #T6778, Sigma) for IF labeling…
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Sympathetic axonopathies and hyperinnervation in the small intestine smooth muscle of aged Fischer 344 rats
Where the paper cites Pel-Freez
…of the nor- adrenaline synthesizing enzyme tyrosine hydroxylase (TH) was used to identify the sympathetic innervation of the jejunum. We used an affinity purified rabbit polyclonal antibody (P40101-0; Pel Freez Biolog- icals, Rogers, AK) specific for the 60 k TH protein. According to the tech- nical information provided by the manufacturer, Western blots are performed on each lot of TH antibody to confirm its…
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An animal model mimicking pedunculopontine nucleus cholinergic degeneration in Parkinson’s disease
Where the paper cites Pel-Freez
…h an antibody directed against NeuN (1:100, monoclonal mouse anti-NeuN, MAB377, Millipore). For doublestaining the SNpc dopa- minergic neurons with aSYN, the sections were first blocked with 20 % NGS in PBS for 60 min, the blocking solution removed, and replaced with a primary antibody solution containing polyclonal rabbit anti-TH (1:100, Pel- freeze) and mouse anti-aSYN (1:100, 610787, BD Trans
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Alpha‐2 adrenergic stimulation triggers Achilles tenocyte hypercellularity: Comparison between two model systems
Where the paper cites Pel-Freez
…g to a novel target for future therapies. The study furthermore shows that animal models are not necessarily required for all aspects of this research. … The primary antibody used to detect TH was supplied by Pel-Freez (P40101, Pel-Freez, Rogers, AR, USA) and used at a concentration of 1:100, the α 2A AR antibody was supplied from…