Yim et al. (2013). Biochemical and Biophysical Research Communications. doi: 10.1016/j.bbrc.2013.07.011
…were dis- sected from 20-day-old flies and fixed with 4% formaldehyde in a fixative buffer. The brains were then stained overnight at 4 C with rabbit anti-tyrosine hydroxylase (TH) antibody (1:100; Pel-Freeze) and mouse anti-TH antibody (1:100; Immunostar). Then, the sam- ples were incubated with Cy3-conjugated secondary antibodies (1:200; Jackson Immunoresearch). The number of DA neurons was counted using a…