Publications
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Ultrasound-Enhanced Delivery of Antibiotics and Anti-Inflammatory Drugs Into the Eye
Where the paper cites Pel-Freez
…nal cells (Gwon 2008), which covers the inner surface of the cornea. In general, the cornea has a higher perme- ability for lipophilic drugs than for hydrophilic drugs (Prausnitz and Noonan 1998). The excised New Zealand White rabbit eyes were purchased from Pel-Freez Biologicals (Rogers, AR, USA). The eyes were stored in Dulbecco modified Eagle medium (DMEM) no more than 24 hours after harvest-
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Three-Dimensional Cell Culture Environment Promotes Partial Recovery of the Native Corneal Keratocyte Phenotype from a Subcultured Population
Where the paper cites Pel-Freez
…twice, and stored in milli-Q. Corneal Cell Isolation Rabbit corneal keratocytes were isolated from rabbit corneas using a previous described protocol.13 Corneas were dissected out of fresh rabbit eyes (Pel-Freeze, Rogers, AK). Following dissection, the concave side of the cornea was incubated in PBS plus typsin from porcine Page 6 of 30 Tissue Engineering Part A Three Dimensional Cell Culture Environment Promotes…
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The α7 nAChR Agonist PNU-282987 Reduces Inflammation and MPTP-Induced Nigral Dopaminergic Cell Loss in Mice
Where the paper cites Pel-Freez
…ine hydroxylase (TH) (rabbit anti-TH; 1: 1000; Pel-Freez Biologicals, Rogers, USA) on day seven after MPTP intoxication (d7). At that time, the number of TH+ cells correlates with the number of Nissl-stained neuronal cells. Seven days post injection, the count of TH+ cells is even more spe- cific and convenient that counting Nissl-stained cells [18]. A biotinylated goat anti-rabbit antibody (1:2
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Sympathetic axonopathies and hyperinnervation in the small intestine smooth muscle of aged Fischer 344 rats
Where the paper cites Pel-Freez
…of the nor- adrenaline synthesizing enzyme tyrosine hydroxylase (TH) was used to identify the sympathetic innervation of the jejunum. We used an affinity purified rabbit polyclonal antibody (P40101-0; Pel Freez Biolog- icals, Rogers, AK) specific for the 60 k TH protein. According to the tech- nical information provided by the manufacturer, Western blots are performed on each lot of TH antibody to confirm its…
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Substratum Compliance Modulates Corneal Fibroblast to Myofibroblast Transformation
Where the paper cites Pel-Freez
…Corp., St. Louis, MO) for 72 hours in medium containing 10% FBS. Isolation and Culture of Primary Stromal Cells From Rabbit Corneas Primary keratocytes were isolated as previously described8 from freshly enucleated young rabbit eyes (Pel-Freez, Rogers, AR), which were examined before dissection and processed within 36 hours of tissue harvest. Cells were cultured in DMEM low glucose supplemented
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Species differences in chlorantraniliprole and flubendiamide insecticide binding sites in the ryanodine receptor
Where the paper cites Pel-Freez
…ies [8]. 2.2. Organisms Live Maine lobsters (Homarus americanus) from a local market were killed by decapitation and the abdominal muscle rapidly ex- cised and frozen in liquid nitrogen then stored at 80 C. Young rabbit muscle tissue from Pel-Freez Biologicals (Rogers, Arkansas, USA) was also stored at 80 C before use. Pupae of Musca and lar- vae of Heliothis and Agrotis were purchased from
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Profiling of lens protease involved in generation of αA-66-80 crystallin peptide using an internally quenched protease substrate
Where the paper cites Pel-Freez
…t to hydrolysis by peptidases found in lenses, which explains their presence in aged lenses and their role in cataract formation. 2. Materials and methods Fresh young bovine lenses were purchased from Pel-Freez Arkansas LLC (Rogers, AR), and human lenses were obtained from the Lions Eye Tissue Bank of Missouri (Columbia, MO). Lenses were stored at 70 C until use. The fluorogenic peptide MCA-SE
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Pneumococcal polysaccharide vaccination induces polysaccharide-specific B cells in adult peripheral blood expressing CD19+CD20+CD3−CD70−CD27+IgM+CD43+CD5+/−
Where the paper cites Pel-Freez
…. Briefly, S. pneumoniae, serotypes 14 and 23F, were incu- bated with serial diluted heat-inactivated sera. Newborn rabbit serum (Pel-Freez, Brown Deer, WI) was added as source of com- plement. Differentiated HL-60 cells were added at an E:T ratio of 400:1. Sera were tested in duplicate. Results were obtained using Opsotiter1 software program (University of Alabama at Birming- ham). 2.6. Statist
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Ocular Disposition of the Hemiglutarate Ester Prodrug of ∆9-Tetrahydrocannabinol from Various Ophthalmic Formulations
Where the paper cites Pel-Freez
…O). Solvents used for analysis were of HPLC grade. Plastic tubes coated with Sigmacote® (Sigma, St. Louis, MO) or boro- silicate glass vials were used for the experiments since THC binds to plastic. Animal Tissues Whole eye globes of New Zealand Albino rabbits were purchased from Pel Freez Biologicals (Rogers, AK). Eyes were shipped overnight in Hanks Balanced Salt Solution over wet ice. Cornea
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Ion Transport Function of SLC4A11 in Corneal Endothelium
Where the paper cites Pel-Freez
…lin (100 U/mL)/fungizone (0.25 lg/mL) mixture in T-25 flasks at 378C with 5% CO2 until confluent, 5 to 7 days. Cells (3.5 3 105) were subcultured into six-well plates or six 25-mm coverslips for experiments, allowed to come to 100% confluence, and used within 24 to 48 hours. Rabbit eyes (Pel-Freez Biologicals, Roger, AR) were used to obtain corneas from which the endothelial layers from the poster
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Flow patterns regulate hyperglycemia-induced subendothelial matrix remodeling during early atherogenesis
Where the paper cites Pel-Freez
…tress Human aortic endothelial cells (HAECs) were purchased from Lonza or Invitrogen at passage 3. Endothelial cells were cultured in either M199 or MCDB131 media supplemented with 10% fetal bovine serum (FBS, HyClone), approximately 0.08e0.17 mg/ml bovine hypothalamus extract (Pel-Freeze), 60 mg/ml heparin (Acros Organics), 100 U/ml penicillin (Hyclone), 100 mg/ml streptomycin (Hyclone), and 2
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Discordant radiologic and histological dimensions of the zone of provisional calcification in fetal piglets
Where the paper cites Pel-Freez
…se of commercially available frozen deceased fetal pigs. The fetal piglet was chosen for this study because of the morphological similarities of some of its osseous structures to those of the human infant [11, 12]. Ten distal tibias (5 right and 5 left) of five deceased frozen near-term mix- breed fetal piglets (Pel-Freez Biologicals, Rogers, AR), weighing 435–680 g (average 516 g), were resect
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Conotoxin truncation as a post-translational modification to increase the pharmacological diversity within the milked venom of Conus magus
Where the paper cites Pel-Freez
…a-MI, des[Gly]1a-MI, and a-MIC. Non-specific binding was determined using 300 mM (–)-nicotine hydrogen tartrate. (ii) a3b4* nAChR: Membranes derived from pig adrenals (6070 mg of protein; Pel-Freez Biologicals, Rogers, AR, USA) were incubated at 22 C for 90 min in 0.5 mL HSS containing 0.5 nM ()-[3H]epibatidine and different con- centrations of conotoxins a-MI, des[Gly]1a-MI, and a-MIC accord
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Anti-dyskinetic effect of the neuronal nitric oxide synthase inhibitor is linked to decrease of FosB/DeltaFosB expression
Where the paper cites Pel-Freez
…nti-TH (1:2000, Pel Freez, Rogers, AR, USA) and rabbit polyclonal anti-FosB/FosB (1:1000, Santa Cruz Biotechnology, USA). Quantifications were carried out using three sections per animal in a light microscope (Leica DMRB) equipped with a video cam- era (Leica DFC420). Neuroanatomical sites were identified using the atlas of Paxinos and Watson [39] and the analysis was done using the software Ima
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An animal model mimicking pedunculopontine nucleus cholinergic degeneration in Parkinson’s disease
Where the paper cites Pel-Freez
…h an antibody directed against NeuN (1:100, monoclonal mouse anti-NeuN, MAB377, Millipore). For doublestaining the SNpc dopa- minergic neurons with aSYN, the sections were first blocked with 20 % NGS in PBS for 60 min, the blocking solution removed, and replaced with a primary antibody solution containing polyclonal rabbit anti-TH (1:100, Pel- freeze) and mouse anti-aSYN (1:100, 610787, BD Trans
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Long-term olive oil-based parenteral nutrition sustains innate immune function in home patients without active underlying disease
Where the paper cites Pel-Freez
…and purified human IgG (Nanogam 50 mg/ml; Sanquin, Amster- dam, The Netherlands) in a 96-well microplate for 30 min at room temperature. Next, neutrophils purified from blood, and rabbit complement (Pel-Freez Biologicals, Rogers, AR, USA) were added and the mixture was incubated for 45 min at room temperature. All of these incubations were performed in triplicate. Subsequently, all samples were spotted in duplicate…
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Transdermal Delivery of Molecules is Limited by Full Epidermis, Not Just Stratum Corneum
Where the paper cites Pel-Freez
…METHODS Porcine Skin Experiments (Without a Mask) Abrasion Protocol All microdermabrasion experiments without the use of a polymer mask were conducted on excised full-thickness adult feeder porcine dorsal skin (2–9 month old, average weight 32 kg, Pel-freeze Biologicals, Rogers, AR) using a Gold Series MegaPeel microdermabrasion machine (DermaMed USA, Lenni, PA) with the gold handpiece assembly
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The mouse dendritic cell marker CD11c is down-regulated upon cell activation through Toll-like receptor triggering
Where the paper cites Pel-Freez
…the medium used was X-VIVO 15 (BioWhittaker) without any serum or plasma. Never- theless, in some cases X-VIVO 15 medium containing 1% or 10% human AB serum (Pel-Freez, Rogers, USA) was used. According to the amount of human serum in medium, it was possible to get CD1a-positive or CD1a-negative MDDC (unpublished observa- tions). All experiments using animals have been approved by the local anim
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The microstructure of collagen type I gel cross-linked with gold nanoparticles
Where the paper cites Pel-Freez
…]. 2.2. Preparation of collagen gels Collagen was extracted from rat-tail tendons (Pel-Freeze Bio- logicals) according to the procedure of Ho et al. [20] lyophilized and stored at 4 ◦C until used. The yield was 29–31%. The collagen was then dissolved in 0.01 M HCl and the purity of the product was examined by SDS–PAGE (Bio-Rad, Mini-Protean TGX). TPAu were coupled to the collagen via EDC (N-(3-
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In utero transplanted human hepatocytes allow postnatal engraftment of human hepatocytes in pigs
Where the paper cites Pel-Freez
…minutes. RPMI-1640 (10 lL) was added to each well, and they were incubated at room temperature for 10 minutes. The supernatant from each well was removed. Two-microliter aliquots of a rabbit comple- ment (Pel-Freez, Rogers, AR) diluted 1:10 in RPMI- 1640 were added to each well, and they were incu- bated at 37C for 30 minutes. A fluorescence-acti- vated cell sorting buffer containing formalin (20 lL) was added to…
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Differential contribution of lipoxygenase isozymes to nigrostriatal vulnerability
Where the paper cites Pel-Freez
…ein concentration was measured by Pierce BCA assay (Thermo Scientific, Rockford, IL) and supernatant proteins separated by SDS–PAGE (12% Tris–glycine, Invitrogen/Life Technologies, Carlsbad, CA) then transferred to nitrocellulose. Blots were blocked in 5% non-fat milk and incubated overnight at 4 C with rabbit anti-tyrosine hydroxylase (TH; Pel-Freez Biologicals, Rogers, AK; and Chemicon/Millip
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CYLD, a deubiquitinase specific for lysine63-linked polyubiquitins, accumulates at the postsynaptic density in an activity-dependent manner
Where the paper cites Pel-Freez
…n from rat cerebral cortices was carried out as described previously [18]. Brains from adult Sprague Dawley rats were collected and frozen in liquid nitrogen within 2 min of decapitation by Pel-Freeze Biologicals (Rogers, AR). Frozen brains were thawed for 1 min in 0.32 M sucrose at 37 C and were imme- diately dissected and homogenized. For rapidly processed PSD preparations, cerebral cortices
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Contrast-enhanced CT with a High-Affinity Cationic Contrast Agent for Imaging ex Vivo Bovine, Intact ex Vivo Rabbit, and in Vivo Rabbit Cartilage
Where the paper cites Pel-Freez
…ence of 1,9-dimethylethylene blue colorimetric assay (10,17) (Appendix E1 [online]). Imaging Protocols for ex Vivo and in Vivo Rabbit Knees Five milliliters of CA4+ and ioxaglate at 12 mg I/mL were injected into contra- lateral intact ex vivo rabbit knees (Pel- Freez Biologics, Rogers, Ark). After 4 hours at room temperature, the joints were scanned by using the same imaging system and paramete
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Alpha‐2 adrenergic stimulation triggers Achilles tenocyte hypercellularity: Comparison between two model systems
Where the paper cites Pel-Freez
…g to a novel target for future therapies. The study furthermore shows that animal models are not necessarily required for all aspects of this research. … The primary antibody used to detect TH was supplied by Pel-Freez (P40101, Pel-Freez, Rogers, AR, USA) and used at a concentration of 1:100, the α 2A AR antibody was supplied from…
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EIF2B and Oligodendrocyte Survival: Where Nature and Nurture Meet in Bipolar Disorder and Schizophrenia?
Where the paper cites Pel-Freez
…d signaling pathways that dictate oligodendrocyte viability and synaptic plasticity. … Spirochetes were cultured in modified Barbour-Stoenner-Kelly (BSK-II) media supplemented with 6% normal rabbit serum (NRS, Pel-Freez 31126-5) and incubated at 37C with 5% CO2…
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Transplantation of intact sheets of fetal neural retina with its retinal pigment epithelium in retinitis pigmentosa patients
Where the paper cites Pel-Freez
…at a single HLA-DR antigen) (see Table 2). The donor DNA was extracted using a DNA tissue extraction kit (QIAGEN, Valencia, California) and typed for HLA-A, -B, and -DR antigens by PCR amplification using sequence specific primers (Pel Freeze, Deer Brown, Wisconsin). Complete preoperative assessments (ocular examina- tion, fluorescein angiography, and mfERG) were repeated after surgery at 1 week,
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Importance of Complement Source in Measuring Meningococcal Bactericidal Titers
Where the paper cites Pel-Freez
…d when serum from a patient with agammaglobulinemia was used (data not shown). The complement source for the standardized assay was from 3- to 4-week-old baby rabbits (Pelfreeze Biologics, Brown Deer, Wis.), and the complement was also qualified based on a lack of intrinsic bactericidal activity and yielded appropriate titers when used as a complement source with various positive- and negative-c
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Human CD8 transgene regulation of HLA recognition by murine T cells.
Where the paper cites Pel-Freez
…addition of target cells using the following mAb: 3.168 (anti-murine CD8), Leu2b (anti-human CD8), and RL172 (anti-murine CD4). Ab was added to effector cells along with guinea pig complement (Pel- Freez Biologicals, Rogers, At O, incubated 1.5 h at 37~ and washed three times. The efficacy of cell depletion was assessed by FACS | analysis to confirm that the appropriate subpopulations were eliminated. Relative…
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Myeloablative therapy with autologous bone marrow transplantation as consolidation of remission in patients with follicular lymphoma
Where the paper cites Pel-Freez
…non-Hodgkin’s lymphoma. The marrow mononuclear cell fraction is being treated in vitro with three cycles of the monoclonal antibody anti-CD20 (anti-B1, Coulter Immunology) and baby rabbit complement (Pel-Freez). Thirty-eight patients with follicular lymphoma (age range 29–61 years, median 43) have been treated to date. At the time of treatment, 28 patients were in second remission, 7 were in third, and 3 were…
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Purification to homogeneity and enzymatic characterization of an alpha-N-acetylgalactosaminide alpha 2 leads to 6 sialyltransferase from porcine submaxillary glands.
Where the paper cites Pel-Freez
…NeuAccu2 + 6GalNAc linkage in both Struc- tures I and II. EXPERIMENTAL PROCEDURES Materials Frozen porcine submaxillary glands were obtained from Pel-Freez Biologicals, Inc. CMP-[‘%]NeuAc was obtained from New England ’ The abbreviations used are: NeuAc, N-acetylneuraminic acid; GalNAc, N-acetylgalactosamine; oSM, ovine submaxillary mucin; the prefix asialo- refers to removal of sialic acid by