Zavalin et al. (2014). Journal of the American Society for Mass Spectrometry. doi: 10.1007/s13361-014-0872-5
…(SA), 1,5-diaminonaphthalene (DAN), chloroform, and methanol were obtained from Sigma, and Indium tin oxide (ITO) coated glass slides from Delta Technologies (Loveland, CO, USA). Frozen mouse brain samples, stored at −80°C were purchased from Pel Freez (Rogers, AR, USA). The tissue sections were cut at 10 μm thickness, thaw-mounted on ITO glass slides, and dried in a vacuum desiccator for 30 mi
Abstract
We have investigated the use of a Gaussian beam laser for MALDI Imaging Mass Spectrometry to provide a precisely defined laser spot of 5 μm diameter on target using a commercial MALDI TOF instrument originally designed to produce a 20 μm diameter laser beam spot at its smallest setting. A Gaussian beam laser was installed in the instrument in combination with an aspheric focusing lens. This ion source produced sharp ion images at 5 μm spatial resolution with signals of high intensity as shown for images from thin tissue sections of mouse brain.