Cascio et al. (2015). Veterinary Immunology and Immunopathology. doi: 10.1016/j.vetimm.2015.04.005
…as described (Cerruti et al., 2007) with minor modifications. Briefly, samples of muscle and spleen from dog and cat were derived from surgical resections, rabbit muscle and spleen were purchased from Pel Freez Biologicals; mouse muscle and spleen were obtained from animals main- tained in the animal facility unit of the SPAE (University of Torino), in conformity with European laws and poli- cies and with the…
Pel-Freez products cited: Rabbit Psoas Muscle, Young, Frozen (41217)
Abstract
Information on the biochemical properties of proteasomes is lacking or, at best, only fragmentary for most species of veterinary interest. Moreover, direct comparison of the limited data available on the enzymatic features of proteasomes in domestic animals is rendered difficult due to the heterogeneity of the experimental settings used. This represents a clear drawback in veterinary research, given the crucial involvement of proteasomes in control of several physiological and pathological processes. We performed the first comparative analysis of key biochemical properties of proteasomes obtained from 8 different domestic mammals. Specifically, we investigated the three main peptidase activities of constitutive and immunoproteasomes in parallel and systematically checked the sensitivity of the chymotryptic site to three of the most potent and selective inhibitors available. Overall, there was substantial similarity in the enzymatic features of proteasomes among the species examined, although some interesting species-specific features were observed.