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Immunohistochemistry and Staining
Whether you’re developing an ELISA-based assay or conducting western blots for basic and discovery research, Pel-Freez can support your work with high-quality antibodies and human and animal blood products.
Because we source raw materials locally and manufacture products in the United States, we are an excellent choice for supply chain security.
Our complete control over the manufacturing process also means we can customize our offering for your specific project needs.
Frequently asked questions
What are the typical dilutions for your Tyrosine Hydroxylase antibodies, such as western blot, ELISA, formalin-fixed paraffin embedded tissue, etc.
A typical starting dilution for antibodies in immunoassays is 1:1000. Consider adjusting the dilution depending on your experimental conditions, to increase or decrease the signal-to-noise ratio, etc.
Should I store antibodies at -20 C or at -80 C? Should I aliquot them upon delivery?
Either - 20 C or - 80 C is acceptable depending on the usage, however, avoid multiple freeze-thaw cycles. Our antibodies contain glycerol which will inhibit freezing at -20 C, however, the materials will freeze at -80 C. If the antibody will be consumed within a few weeks of delivery, we recommend storage at -20 C. For long term storage, we recommend subaliquoting in single-use aliquots then storing at -80 C.
Research citing Antibodies
8 peer-reviewed papers that used a product from this category, newest first.
All publications-
A novel rat model of body first subtype of Parkinsons disease
Where the paper cites Pel-Freez
…for 1 hour at room temperature to block nonspecific binding sites and then incubated in primary antibody (rabbit anti- tyrosine hydroxylase, Pel Freez Biologicals #P40101-150, 1:250 in blocking solution) for 72 hours at 4oC on a shaker. Sections were washed in between incubation steps with 6 x 3min PBS-Tx. Sections were incubated in diluted secondary antibody (biotinylated donkey anti-rabbit Ig
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High molecular weight insoluble parkin in the substantia nigra of patients with idiopathic Parkinson’s disease
Where the paper cites Pel-Freez
…1 h at ambient temperature. As for the immunoblots, the antibodies against parkin C-Terminal region (monoclonal parkin clone [PRK8], 1:1,000 and polyclonal parkin (cell signaling cat#2132, 1:1,000)), TH (Pel-Freez Biolo- gicalsCat# P40101-150, 1:1,000),αsyn(SYN1,BDBiosciencesCat# 610787, 1:1,000), MJFR1 (Abcam Cat# ab138501 [MJFR1], 1:1,000) and αsyn phosphorylatedatserine129(αsynP129)(AbcamCat#ab168381[MJF-R13…
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Integration of cross-species multi-omics with in vivo experimental validation identifies Parkinson's disease therapeutic targets and novel risk factors within endolysosomal pathway subnetworks
Where the paper cites Pel-Freez
…times for 5 min each using 1× PBST. Samples were placed in blocking buffer (1% bovine serum albumen in PBST) for 1 h at room temperature and then incubated with anti-Tyrosine Hydroxylase (1:500, P40101 PelFreez) for 3 days at 4 °C. After the primary was removed, samples were washed 3 times with 1× PBST for 5 min each, then incubated in secondary (1:500 Alexa Fluor anti-Rabbit 555 (A-21428) and 1:1000 DAPI) for 1…
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Loss of PINK1 causes age-dependent mitochondrial trafficking deficits in nigrostriatal dopaminergic neurons via aberrant p38 MAPK activation
Where the paper cites Pel-Freez
…an investigator blinded to the genotype. ARTICLE IN PRESS ARTICLE IN PRESS Antibodies and Reagents The antibodies and reagents used in the study include: Anti-Tyrosine Hydroxylase (rabbit, 1:5000 for IF, Pel-Freez, P40101-0), Anti-Tyrosine Hydroxylase (mouse, 1:500 for IF, Millipore, MAB5280), Anti-Kinesin 5B heavy chain (mouse, 1:500 for WB, Millipore, MAB1614), Anti- Dynein medium chain (mouse, 1:1000 for WB,…
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Miro1 mutations disrupt cellular calcium homeostasis via dysregulation of mitochondria-ER-contact-sites, rendering iPSC-derived neurons more susceptible to lipid peroxidation
Where the paper cites Pel-Freez
…Peprotech, 450-10-50UG). Neuronal cultures were assessed by immunostaining with antibodies against Mikrotubuli-associated protein 2 (MAP2; Abcam ab5392, dilution 1:10000) and Tyrosin Hydroxylase (TH; Pel-Freez P40101, dilution 1:1000). 2.2 Live cell imaging of mitochondrial calcium Neurons were cultivated in 8 well chamber slides (Ibidi, 80806) at 100,000 cells per well. Then, neurons were transfected with 0.5…
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Targeting reverse electron transport preserves mitochondrial function and prevents dopaminergic neurodegeneration in Parkinson’s disease
Where the paper cites Pel-Freez
…then incubated at 4 °C overnight with a rabbit polyclonal primary antibody against TH, GFAP or IBA-1 in KPBS containing 0.4% triton X-100 (TH, Pel Freez #P40101- 150, 1:1000; GFAP, Proteintech #16,825–1-AP 1:500; and Iba-1, Novus biologicals Cat#NBP2-19,019, 1:500). After five to six rinses in KPBS, the sections were treated for two hours in a biotinylated secondary antibody (PK- 6200, 1:1000,
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