Publications
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RGS14 modulates locomotor behavior and ERK signaling induced by environmental novelty and cocaine within discrete limbic structures
Where the paper cites Pel-Freez
…buffer, including primary antibodies raised against pERK (rabbit an- ti-Phospho-p44/42 MAPK, Cell Signaling Technology, Danvers, MA, #4370; 1:1000) or TH (rabbit anti-TH, P40101-0, Pel-Freez, Rogers, AR; 1:1000) or STEP (rabbit anti-Non-phospho-STEP, Cell Signaling Technology, #5659; 1:500) and RGS14 (mouse anti-RGS14, NeuroMab, Davis, CA, clone N133/21; 1:500). The sections were then…
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Electromagnetized Graphene Facilitates Direct Lineage Reprogramming into Dopaminergic Neurons
Where the paper cites Pel-Freez
…ostaining, brains were stain with anti-TH antibody (1:1000, Pel-Freez) for 24 h at 4 °C and then incubated with secondary Alexa 488 (Invitrogen) and DAPI. All brain sections were visualized under a Nikon confocal microscope. For DAB staining, brains were stained with anti-DAT antibody (1:1000, Pel-Freez) for 24 h at 4 °C and then incubated in/with antirabbit Ig HRP (GenDEPOT, City, State, Country). Brain section…
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Variable Autoinhibition among Deafness-Associated Variants of Diaphanous 1 (DIAPH1)
Where the paper cites Pel-Freez
…To isolate actin, 3 g of rabbit muscle acetone powder (Pel Freez Biologicals) was mixed with 100 mL of actin extraction buffer (2 mM Tris-HCl, 0.2 mM CaCl 2 , 0.2 mM ATP, and 1 mM…
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The contribution of TFPIα to the hemostatic response to injury in mice
Where the paper cites Pel-Freez
…(ThermoScientific, Waltham, MA). Samples with or without addition of 50 nM polyclonal rabbit anti-mouse TFPI antibody 23,39,41,46–48 were incubated for 20 min with 1:500 rabbit brain cephalin (Pel-Freez Biologicals, Rogers, AK), 0.001 nM human FVIIa (Novo Nordisk A/S, Bagsvaerd, DK) and 1:4000 human TF (Siemens, Marburg, Germany). Spectrozyme FXa (0.5 mM, Sekisui Diagnostics, Stamford, CT) was added and…
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Synthetic DNA Delivery of an Optimized and Engineered Monoclonal Antibody Provides Rapid and Prolonged Protection against Experimental Gonococcal Infection
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Salic acid protects nontypeable Haemophilus influenza from natural IgM and promotes survival in murine respiratory tract.
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Performance of a Four-Antigen Staphylococcus aureus Vaccine in Preclinical Models of Invasive S. aureus Disease
Where the paper cites Pel-Freez
…immune sera were mixed with either a CP5-expressing or CP8-expressing clinical isolate of S. aureus and allowed to opsonize the bacteria. The reaction mixtures were then mixed with baby rabbit complement (Pel-Freez, Rogers, AR, USA) and neutrophil-like HL-60 cells (ATCC ® CCL-240™, Manassas, VA, USA). An OPA antibody titer was defined as the reciprocal of the highest serum dilution resulting in 50% reduction of the…
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Opsonophagocytic Killing Assay to Measure Anti-Group A Streptococcus Antibody Functionality in Human Serum
Where the paper cites Pel-Freez
…≤ 1 month. 10. Opsonisation buffer (OPS buffer), 5ml FCS, 40ml 1x HBSS (+Ca/Mg), 5ml 1% gelatin solution. Prepare on day of assay and discard after use. 11. HL-60 Cells, ATCC. 12. Baby rabbit complement, Pel-Freez. Page 5 of 22 3. Methods 3.1. HL-60 Cells. HL-60 cells are promyelocytic leukaemia cells which are differentiated to a neutrophil-like cell with 0.8% dimethylformamide (DMF). All cell culture is to be…
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Opsonophagocytic Antibodies of Intravenous Immunoglobulin Preparations against Seven Streptococcus agalactiae Serotypes
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Myeloperoxidase has no effect on the low procoagulant activity of silica-free DNA.
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Multicomponent Vaccines against Group A Streptococcus Can Effectively Target Broad Disease Presentations
Where the paper cites Pel-Freez
…penicillin–streptomycin and differentiated with 0.8% DMF at 4 × 10 5 cells/mL in 100 mL for 5 to 6 days. Frozen GAS cultures were tested with differentiated HL60 cells and 2% baby rabbit complement (Pel-Freez Biologicals) or heat inactivated complement at a bacteria:cell ratio of 1:400 with <35% nonspecific killing [ 13 ]. To perform the OPA, GAS frozen cultures were thawed, washed and diluted to the required input…
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Modelling folates reaction kinetics during cowpea seed germination in comparison with soaking
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Limited Expansion of Human Hepatocytes in FAH/ RAG2 -Deficient Swine
Where the paper cites Pel-Freez
…according to the manufacturer protocol for 24–96 h, then incubated for 30 min with pig serum diluted 1:8 in manufacturer-recommended media. Cells were washed and incubated for 1 h with rabbit complement (Pel-Freez, Rogers, AR) diluted 1:16 in media. FlouroQuench (Thermo Fisher Scientific) was applied to each well. For comparisons of complement cytotoxicity between different assays, the percentage of cell death…
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Isolation and Characterization of Human Monoclonal Antibodies to Pneumococcal Capsular Polysaccharide 3
Where the paper cites Pel-Freez
…were diluted 3-fold from 20 mg/ml. ST3 (A66) killing (%) was determined in the presence of humAbs under the following two conditions: with HL60 cells and complement (3- to 4-week rabbit complement; Pel-Freez) or without HL60 cells (humAbs and bacteria only) by plating aliquots of samples onto blood agar plates and enumerating CFU. In vitro bacterial gene expression by reverse transcription-quantitative PCR…
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Immunogenicity of PCV24, an expanded pneumococcal conjugate vaccine, in adult monkeys and protection in mice.
From the abstract
Invasive pneumococcal disease (IPD) is responsible for serious illnesses such as bacteremia, sepsis, meningitis, and pneumonia in young children, older adults, and persons with immunocompromising conditions and often leads to death. Although the most recent pneumococcal conjugate vaccines (PCVs) have been designed to target serotypes identified as the primary causative agents of IPD, the epidemiological landscape continues to change stressing...
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Fyn knockdown prevents levodopa-induced dyskinesia in a mouse model of Parkinson's disease
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Fascin-induced actin protrusions are suppressed by dendritic networks in GUVs.
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Expression of Bordetella pertussis Antigens Fused to Different Vectors and Their Effectiveness as Vaccines
Where the paper cites Pel-Freez
…100–200 colony- forming units (CFU)/10 µL. Next, 10 µL of diluted bacterial suspension was mixed with 10 µL of serially diluted serum (preheated in a 58 ◦C water bath for 30 min to inactivate complement (Pel-Freez) and 20 µL of complement. After incubation at 37 ◦C for 1 h, the mixture was coated onto plates containing pertussis charcoal medium and further Vaccines 2021, 9, 542 5 of 14 incubated for 4–5 days at 37…
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Expression of Acyl-CoA wax-alcohol acyltransferase 2 (AWAT2) by human and rabbit meibomian glands and meibocytes
Where the paper cites Pel-Freez
…in staining intensity. Lipid content per cell was measured as described previously [23]. 2.5. Immunocytochemistry For analysis of intact meibomian glands, eyelids were obtained from 8-week-old rabbits (Pel Freez) within 24 h after death. Upon arrival at the lab, tissue was immediately dissected and then embedded in O.C.T. Compound, frozen in liquid nitrogen, and stored at −80 °C. Blocks were then sectioned using…
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Evaluation of Critical Quality Attributes of a Pentavalent (A, C, Y, W, X) Meningococcal Conjugate Vaccine for Global Use
Where the paper cites Pel-Freez
…Pathogens 2021, 10, 928 15 of 17 To determine rSBA titers, individual rabbit sera were inactivated at 56 ◦C before initiating the assay. The complement source was 3–4 week old rabbit complement (Pel- freez Biologicals, Rogers, AR, USA). The bacterial strains used in the rSBA assays were: serogroup A, CDC F8238, phenotype A:4:P1.9, O-Acetylation OAc+; serogroup C, strain C11, C:16:P1.7–1,1.1, OAc+; serogroup Y,…
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Effect of supersaturated oxygen emulsion treatment on chloropicrin-induced chemical injury in ex vivo rabbit cornea.
From the abstract
With a possibility for the use of chemical weapons in battlefield or in terrorist activities, effective therapies against the devastating ocular injuries, from their exposure, are needed. Oxygen plays a vital role in ocular tissue preservation and wound repair. We tested the efficacy of supersaturated oxygen emulsion (SSOE) in reducing ex vivo corneal and keratocyte injury from chloropicrin (CP). CP,...
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Development and Immunogenicity of a Prototype Multivalent Group B Streptococcus Bioconjugate Vaccine
Where the paper cites Pel-Freez
…Bio-Rad). The samples were subsequently transferred to nitrocellulose membranes with a 0.2 μm pore size (Bio-Rad… Baby rabbit complement (Pel-Freez) was added to HL-60 cells at a 1…
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Development and Immunogenicity of a Brazilian Glycoconjugate vaccine against Meningococcal W in a Pilot Scale
Where the paper cites Pel-Freez
…Pel-Freez® baby rabbit complement lacking bactericidal activity was added. After incubation 10 µl of the mixture from each well was plated out on Columbia blood agar plate by the tilt…
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Design of Topical Ocular Ciprofloxacin Nanoemulsion for the Management of Bacterial Keratitis
Where the paper cites Pel-Freez
…3.2.9. Ex Vivo Transcorneal Permeation Studies Ex vivo transcorneal permeation studies for test and control formulations were per- formed on corneas isolated from rabbit whole eyes, shipped from Pel-Freez Biologicals (Rogers, AR, USA). The eyes were preserved in Hanks’ balanced salt solution in ice and shipped overnight. The corneas were carefully excised and used for the permeation studies immediately upon…
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Broadly Reactive Human Monoclonal Antibodies Targeting the Pneumococcal Histidine Triad Protein Protect against Fatal Pneumococcal Infection
Where the paper cites Pel-Freez
…were mixed with HBSS as a negative control, and purified human serum IgG was used as a positive control. Differentiated HL-60 cells were washed twice with HBSS and mixed with baby rabbit complement (Pel-Freez Biologicals) at a final concentration of 10% in each well. Following this, 60ml (1 106 viable cells) of differentiated HL60 cells and complement were added to the mixture of bacteria and antibodies and…
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A Structural Model for the Ligand Binding of Pneumococcal Serotype 3 Capsular Polysaccharide-Specific Protective Antibodies.
Where the paper cites Pel-Freez
…and 1% L-glutamine. HL-60 cells were differentiated using 0.6% N,N-dimethylformamide (DMF) for 3 days before performing the OPKA assay, harvested, and resuspended in OBB. Baby rabbit complement (Pel- Freez) was added to HL-60 cells at a 1:5 final volume. The HL-60-complement mixture was added to the bacteria at 5 105 cells/well. The final reaction mixtures were incubated at 37°C for 1 h with shaking. The…
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3D Printable, Modified Trephine Designs for Consistent Anterior Lamellar Keratectomy Wounds in Rabbits.
Where the paper cites Pel-Freez
…51 μm were obtained from McMaster-Carr, trephine blades with 3.5 mm diameter (Item # 33–0350) were purchased from Ambler surgi cal. Rabbit eyes were obtained from VisionTech (Sunnyvale, Texas) and Pel-Freez Biologicals (Rogers, Arkansas). Pig eyes were obtained from VisionTech (Sunnyvale, Texas). Trephine parts designing and prototyping SolidWorks®, a 3-Dimensional computer-aided design (3D-CAD) software, was…
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The Dual Role of a Polyvalent IgM/IgA-Enriched Immunoglobulin Preparation in Activating and Inhibiting the Complement System
Where the paper cites Pel-Freez
…serum was used as a source of complement. Furthermore, to investigate the role of the Igs in trimodulin, serum depleted for IgM and IgG was used (NHS min ; normal human serum depleted of IgG and IgM, Pel-Freez, Rogers, USA), since serum depleted for IgM, IgA and IgG (Sigma-Aldrich, St. Louis, MO, USA) was found to be deficient in C1q [ 50 ]. HL60 cells (ATCC ® CCL240™, Manassas, VA, USA) served as effector…
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Meningococcal Detoxified Outer Membrane Vesicle Vaccines Enhance Gonococcal Clearance in a Murine Infection Model
Where the paper cites Pel-Freez
…pooled from 4–6 mice immunized with dOMVs or Alum were tested similarly. The active complement source was pooled immunoglobulin G (IgG)/immunoglobulin M–depleted human serum (gen- erous gift of Pel-Freez Biologicals) at 25% final concentration; heat-inactivated complement was tested in parallel as a nega- tive control. After 45 minutes, aliquots were plated in triplicate on GCB agar and incubated overnight.…
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Actin bundle architecture and mechanics regulate myosin II force generation
Where the paper cites Pel-Freez
…Rabbit skeletal muscle is purified from acetone powder (Pel-Freez Biologicals, Rogers, AR) as described in (21). Actin is visualized by incorporating ∼25% actin monomers that were…