Publications

272 publications

  1. 2021Choi et al. (2021). Animal Cells and Systems.

    Physicochemical and biological similarity assessment of LBAL, a biosimilar to adalimumab reference product (Humira®)

    Where the paper cites Pel-Freez

    …of neutralization was calculated from the dose −response curve. Complement-dependent cytotoxicity (CDC) Samples were incubated with the tmTNF-CHO cells in the presence of rabbit complement serum (Pel-Freez Biologicals, USA) at 37°C for 2 h to induce cell lysis. The cell viability was analyzed by the MTS assay, and the percentage of cytotoxicity was calculated from the dose–response curve. Ab-dependent cellular…

  2. 2021Fernandez et al. (2021). The Journal of Infectious Diseases.

    Vaccination with Detoxified Leukocidin AB Reduces Bacterial Load in a Staphylococcus aureus Minipig Deep Surgical Wound Infection Model

  3. 2020Kim et al. (2020). Neuropharmacology.

    Nrf2 activator via interference of Nrf2-Keap1 interaction has antioxidant and anti-inflammatory properties in Parkinson's disease animal model

    Where the paper cites Pel-Freez

    …Immunostaining The brain sections were rinsed in PBS and incubated in 3% hy- drogen peroxide for 15 min. Subsequently, the sections were treated with anti-tyrosine hydroxylase antibody (1:500, Pel- Freez Biologicals, Rogers, AR, USA) overnight at 4 °C and then with a secondary antibody, Polink-2 Plus HRP Broad for mouse and rabbit (GBI Labs, Bothell, WA, USA), in accordance with the manufacture's instructions.…

  4. 2020Goggi et al. (2020). Stem Cell Research & Therapy.

    Dopamine transporter neuroimaging accurately assesses the maturation of dopamine neurons in a preclinical model of Parkinson’s disease

    Where the paper cites Pel-Freez

    …of 3% donkey serum (Sigma-Aldrich, USA), 2% CellMaxx™BSA (MP Biomedicals, USA), 0.1% Triton (Sigma-Aldrich, USA) in PBS for at least 1 h be- fore incubating with primary antibodies (1:500 rabbit anti-TH (Pel-Freez, USA) and 1: 1000 mouse anti-Tuj1 (Covance, USA); all diluted in blocking solution) over- night at 4 °C. Cells were incubated with secondary anti- bodies (1:500 donkey anti-rabbit 488 and 1:500 donkey…

  5. 2020Shaaban et al. (2020). Nature Structural & Molecular Biology.

    Cryo-EM reveals the transition of Arp2/3 complex from inactive to nucleation-competent state

    Where the paper cites Pel-Freez

    …Nature Structural & Molecular BioloGy | www.nature.com/nsmb Articles Nature Structural & Molecular Biology Methods Protein purification. Rabbit skeletal muscle actin was extracted from acetone powder (Pel Freeze, catalog no. 41995) by stirring in G-buffer (20 ml g–1, 2 mM Tris pH 8.0, 0.2 mM ATP, 0.5 mM DTT, 0.1 mM CaCl2) on ice for 30 min before spinning in a Beckman JA-20 rotor at 16,000 r.p.m. for 30 min at 4…

  6. 2020Narayanan et al. (2020). Biochemical and Biophysical Research Communications.

    Zika Virus-Like Particle (VLP) vaccine displaying Envelope (E) protein CD loop antigen elicits protective and specific immune response in a murine model

    Where the paper cites Pel-Freez

    …Zika virus strain PRVABC59 at MOI of 0.1, 2 days prior to the assay. Infected and uninfected target cells were incubated with mouse serum samples diluted 1:10 in the presence of rabbit complement (1:20) (Pel-Freez Biological) for 90 min under standard culture conditions. Cell toxicity was measured using the CytoTox-Glo™Cytotoxicity Assay (Promega) and by luminescence plate reader (Perkin Elmer). CDC induction was…

  7. 2020Vinhas et al. (2020). Journal of Vaccines and Immunology.

    Validity of Enzyme-Linked Immunosorbent Assay (ELISA) as a correlate of protection of meningococcal C conjugated vaccine in adolescents with HIV infection

    Where the paper cites Pel-Freez

    …SBA The bactericidal activity of the anti-meningococcal serogroup C antibodies was measured by the SBA test, using an external source of complement (3- to 4-week baby rabbit complement sterile, Pel-Freez Biologicals Division, AR). This test was standardized at a reference laboratory in Brazil. Internationally accepted protocols, based on two studies by Borrow et al., and one by Maslank et al., were used…

  8. 2020Brekk et al. (2020). Acta Neuropathologica Communications.

    Upregulating β-hexosaminidase activity in rodents prevents α-synuclein lipid associations and protects dopaminergic neurons from α-synuclein-mediated neurotoxicity

    Where the paper cites Pel-Freez

    …Triton-X, blocked in 10% normal goat serum in PBS 0.3% Triton-X, and primary antibodies were applied overnight at 4C. Primary antibodies used were rabbit anti-tyrosine hydroxylase (Pel-Freez Biologi- cals Cat# P40101, RRID:AB_2313713), mouse anti-aSYN (human) (Millipore Cat# 36–008, RRID:AB_310817), mouse anti-aSYN (pan species) (BD Biosciences Cat# 610786, RRID:AB_398107), mouse anti-LAMP-1 (S

  9. 2020Scholz, M. et al. Tuning molecular motor transport through cytoskeletal filament network organization. Soft Matter 16, 2135-2140 (2020).

    Tuning molecular motor transport through cytoskeletal filament network organization

    Where the paper cites Pel-Freez

    …in terms of the known features of the crosslinking proteins. Materials and methods Protein and vesicle preparation Monomeric actin was purified from rabbit skeletal muscle acetone powder (Pel-Freez Biologicals) using a protocol adapted from ref. 30. For imaging with fluorescence microscopy, actin monomers were labeled with the fluorophore tetramethyl- rhodamine-6-maleimide (TMR; Life Technologies). Both…

  10. 2020Yu et al. (2020). Colloids and Surfaces A Physicochemical and Engineering Aspects.

    Transport of polymers in contact lenses and impact on lubricity

    Where the paper cites Pel-Freez

    …een the lens surface and a rabbit cornea in a lab-designed apparatus. Fig. 1A shows a picture of the lubricity testing device. A rabbit cornea was excised from a cadaver rabbit eye provided by Pel-Freez and adhered to the top of a hemispherical domed rod using Pelco® Pro CA44 instant tissue adhesive. The domed rod was clamped in the center of a SHIMPO TNP0.5 0.5 Capacity Torque Meter. Different

  11. 2020von Herrmann et al. (2020). Journal of Neuroinflammation.

    Slc6a3-dependent expression of a CAPS-associated Nlrp3 allele results in progressive behavioral abnormalities and neuroinflammation in aging mice

    Where the paper cites Pel-Freez

    …(AL177, AdipoGen Life Science, San Diego, CA), interleukin-1 beta (IL-1β) (AF-401-NA, R&D Systems, Minneapolis, MN), and tyrosine hydroxylase (TH) (P40101, Pel-Freez Biologicals, Rogers, AR). IHC studies were conducted with either BOND polymer refined de- tection system (Leica Biosystems, Wetzlar, Germany) or HRP Polymer (Biocare Medical, Pacheco, CA). Images were obtained using an Olympus BX51

  12. 2020Liang et al. (2020). Journal of Drug Delivery Science and Technology.

    Optimization of dip-coating methods for the fabrication of coated microneedles for drug delivery

  13. 2020Chin et al. (2020).

    N-terminal modification of actin by acetylation and arginylation determines the architecture and assembly rate of linear and branched actin networks

  14. 2020Sung et al. (2020). Journal of Autoimmunity.

    Interactions among glomerulus infiltrating macrophages and intrinsic cells via cytokines in chronic lupus glomerulonephritis

  15. 2020Trevijano-Contador et al. (2020). Infection and Immunity.

    Human IgM inhibits the formation of titan-like cells in Cryptococcus neoformans

  16. 2020LaGrow et al. (2020). Microorganisms.

    Expression of Bacillus cereus Virulence-Related Genes in an Ocular Infection-Related Environment

    Where the paper cites Pel-Freez

    …brain heart infusion (BHI) broth, LB broth, or ex vivo rabbit vitreous and incubated at 37 ◦C for 18 h. Rabbit vitreous was prepared from batches of 50 mature rabbit eyes obtained from Pel-Freez Biologicals (Rogers, AR, USA). An incision was made in the corneal limbus, the aqueous humor and lens discarded, and the vitreous collected from each eye. Vitreous from all 50 eyes was pooled and…

  17. 2020Bradford et al. (2020). Translational Vision Science & Technology.

    Enhanced Transepithelial Riboflavin Delivery Using Femtosecond Laser-Machined Epithelial Microchannels

    Where the paper cites Pel-Freez

    …ave the added advantage of being highly compatible with NLO CXL after Rf application, which can produce CXL at any depth and pattern. Methods Tissue Preparation A total of 64 ex vivo, intact rabbit eyes were shipped overnight (Pel-Freez, Rogers, AR) and prepared for use upon arrival as previously reported.5,6,35 Briefly, they were rinsed in phenol-free, low-glucose, Dulbecco’s modified Eagle’s

  18. 2020Wang et al. (2020). The AAPS Journal.

    Development of a Highly Specific Anti-drug Antibody Assay in Support of a Nanoparticle-based Therapeutic

  19. 2020Kassumeh et al. (2020). Translational Vision Science & Technology.

    Corneal Stromal Filler Injection as a Novel Approach to Correct Presbyopia—An Ex Vivo Pilot Study

    Where the paper cites Pel-Freez

    …central refractive power (Fig. 1). We proposed that the anterior corneal curvature and peripheral posterior curvature would remain unaffected by the procedure. Corneal Tissue Preparation New Zealand White rabbit cadaver eyes were obtained from Pel-Freez Biologicals (Rogers, AR) and processed within 24 hours post mortem. To dehydrate the corneas, they were kept in phosphate-buffered saline (PBS;

  20. 2020Wertheimer et al. (2020). European Journal of Ophthalmology.

    Corneal optical density in Fuchs endothelial dystrophy determined by anterior segment optical coherence tomography

    Where the paper cites Pel-Freez

    …ver globes (Pel freez, Rogers, AR, USA) within 24 h of death. Corneoscleral discs with a diameter of about 17 mm were excised using keratoplasty scissors (Katena, Denville, NJ, USA). The discs were placed in PBS (Fisher scientific, Pittsburgh, PA, USA) containing 20% w/v Dextran (Mr 450,000–600,000, Sigma, St. Louis, MO, USA) for at least 30 min, even longer if corneal pachymetry was not below

  21. 2020Li et al. (2020). Journal of Materials Chemistry B.

    Continuous manufacturing of uniform chitosan beads as hemostatic dressings by a facile flow injection method

  22. 2020Liu et al. (2020). Frontiers in Molecular Biosciences.

    Confinement Geometry Tunes Fascin-Actin Bundle Structures and Consequently the Shape of a Lipid Bilayer Vesicle

    Where the paper cites Pel-Freez

    …for studying cytoskeleton-membrane interactions and cellular shape changes. MATERIALS AND METHODS Preparation of Proteins and Reagents We purified actin from rabbit skeletal muscle acetone powder (Pel-Freez Biologicals) as described previously (Pardee and Aspudich, 1982) or purchased it from Cytoskeleton Inc, United States. ATTO 488 actin was purchased from Hypermol Inc, Germany. α-Actinin was purchased from…

  23. 2020Brandt et al. (2020). Translational Vision Science & Technology.

    Arginine as an Enhancer in Rose Bengal Photosensitized Corneal Crosslinking

    Where the paper cites Pel-Freez

    …of this process and to test a method for excluding oxygen that can be used in the clinic. Methods Tissue Preparation Mature New Zealand white rabbit eyes freshly frozen were purchased from Pel-Freez Biologicals (Rogers, AR) and prepared as described previously.9 In brief, after removal of the epithelium, a corneoscle- ral disc was prepared and, for previously frozen eyes, placed in phosphate buffered…

  24. 2020Cota-Coronado et al. (2020). STAR Protocols.

    An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions

    Where the paper cites Pel-Freez

    …Cruz Biotechnology Sc-990, RRID:AB_2298676 Goat polyclonal anti-OTX2 R&D Systems AF1979, RRID:AB_2157172 Sheep polyclonal anti-PITX2 R&D Systems AF7388, RRID:AB_11128639 Rabbit polyclonal anti-TH Pel-Freez Biologicals P40101-0, RRID:AB_461064 Sheep polyclonal anti-TH Pel-Freez Biologicals P60101-0, RRID:AB_461070 Chemicals, Peptides, and Recombinant Proteins Accutase Innovative Cell Technologies AT104 Ascorbic…

  25. 2020Liu et al. (2020). Frontiers in Medicine.

    Role of HK2 in the Enzootic Cycle of Borrelia burgdorferi

  26. 2020Bowman et al. (2020). Translational Vision Science & Technology.

    Intrastromal injection of hyaluronidase alters the structural and biomechanical properties of the corneal stroma

    Where the paper cites Pel-Freez

    …properties and collagen fibril spacing of its stroma in vivo. Materials and Methods Cell Isolation, Culture, and Viability Test Primary keratocytes were isolated from freshly enucleated young rabbit eyes (Pel-Freez Biologi- cals, Rogers, AR) as previously described.9 Cells were cultured in low-glucose Dulbecco’s modified Eagle’s medium (Gibco BRL, Grand Island, NJ) supplemented with 10% fetal bovine serum and 1%…

  27. 2020Youssef et al. (2020). Pharmaceutics.

    Ciprofloxacin loaded nanostructured lipid carriers incorporated into in-situ gels to improve management of bacterial endophthalmitis

    Where the paper cites Pel-Freez

    …Permeation Studies Ex vivo transcorneal permeation of CIP from CIP-NLCs and CIP-NLC-IG formulations, in comparison with CIP-C, were studied on corneas excised from rabbit whole eyes, shipped from Pel-Freez Biologicals (Rogers, AR, USA). The eyes were stored in Hanks’ balanced salt solution under cold conditions and shipped overnight. The corneas were carefully excised and used for the permeation studies…

  28. 2020Goode et al. (2020). The Journal of Cell Biology.

    Twinfilin bypasses assembly conditions and actin filament aging to drive barbed end depolymerization

    Where the paper cites Pel-Freez

    …and methods Purification and labeling of actin Rabbit skeletal muscle actin was purified from acetone powder (Spudich and Watt, 1971) generated from frozen ground hind leg muscle tissue of young rabbits (Pel-Freez). Lyophilized acetone powder stored at −80°C was mechanically sheared in a coffee grinder, resuspended in G-buffer (5 mM Tris-HCl pH 7.5, 0.5 mM DTT, 0.2 mM ATP, and 0.1 mM CaCl2), and then cleared by…

  29. 2020Jha et al. (2020). Euphytica.

    Folate profile diversity and associated SNPs using genome wide association study in pea

  30. 2020Baik et al. (2020). Frontiers in Bioengineering and Biotechnology.

    Quality improvement of capsular polysaccharide in streptococcus pneumoniae by purification process optimization

    Where the paper cites Pel-Freez

    …and differentiated into granulocytes with dimethylformamide. HL-60 cells were allowed to phagocytose bacteria in the presence of sera containing anticapsular antibodies and baby rabbit complement (Pel-Freez Biologicals). The assay was performed on microtiter plates, and duplicate samples from each well were plated on THYE agar plates. The results were expressed as an opsonic index, which is the reciprocal of the…