Publications
-
Impulsive-compulsive behaviours and striatal neuroactivity in mildly parkinsonian rats under D2/3 agonist and L-DOPA treatment
Where the paper cites Pel-Freez
…analysis. For quantifying the extent of striatal dopaminergic denervation and counting of pS6 + cells, bright-field immunohistochemistry was performed using a primary antibody against TH (rabbit anti-TH, Pel-Freez P40101, 1:1000) and phosphorylated pS6, respectively (monoclonal rabbit anti Ser235/236-phospho-S6, Cell Signaling #2211, 1:200). Immunocomplexes were revealed using biotinylated secondary antibodies…
-
Immunogenicity of primary and booster MenACWY-TT vaccination in older adults and the importance of IgM
Where the paper cites Pel-Freez
…Frailty Indicator self-report questionnaire35. Serological analysis Functional MenACWY-specific antibody titers were determined using the serum bactericidal assay with baby rabbit complement (rSBA) (Pelfreez, lot: 21044-C) as described previously15,36. Target strains included MenA (strain 3125), MenC (strain C11), MenW (strain MP01240070), and MenY (strain S-1975). rSBA titers were expressed as the reciprocal…
-
Idiopathische eosinophile Meningoenzephalomyelitis bei einer jungen belgischen Tervueren-Schäferhündin
Where the paper cites Pel-Freez
…Purified P and H (Quidel, San Diego, CA), anti-C3 (Dako, Glostrup, Denmark), anti-B (Dako), cobra venom factor (Diamedix), rat serum (Calbiochem), and guinea pig serum (Pel-Freez…
-
Hemoglobin as a pseudoperoxidase and drug target for oxidative stress-related diseases
Where the paper cites Pel-Freez
…anti-Hbβ (1:200, Abcam, ab214049), mouse anti-Hbβ (1:200, Santacruz, sc-21757), mouse anti-MAOB (1:200, Santacruz, sc-515354), mouse anti-8-OhdG (1:500, Santacruz, sc-393871), rabbit anti-TH (1:500, Pelfreez, p40101), and mouse anti-TH (1:200, Sigma, T2928). The following secondary antibodies from Jackson were diluted (1:500) in blocking solution: donkey-anti-chicken 405, donkey-anti-chicken 647,…
-
Eplets, Epitopes, and Antibody Verified Eplets in Clinical Transplantation
Where the paper cites Pel-Freez
…ve benefit. Transplant Proc. 1997;29(1–2):1435–8. Article CAS PubMed Google Scholar Wujciak T, Opelz G. Evaluation of HLA matching for CREG antigens in Europe1. Transplantation. 1999;68(8):1097–9. Article CAS PubMed Google Scholar Rodey G. HLA beyond tears: introduction to human histocompatibility. 2nd ed. Durango, CO: De Novo: Distributed by Pel-Freez; 2000. Google Scholar McCaughan J, Xu Q, T
-
Elevated plasma levels of alpha-synuclein are dispensable for Parkinson’s disease pathology
Where the paper cites Pel-Freez
…ase (1:500, Pel-Freez Biologicals, #P60101-150) and anti-CD31 (1:150, BD Bioscience, #553370). To reduce nonspecific autofluorescence in aged tissue samples, sections were treated with 1× TrueBlack solution (Biotium, #23007) diluted in 70% ethanol for 30 s, followed by thorough PBS washes. Images were acquired with a Leica TCS SP8 confocal microscope (Leica, Germany) and NiKon A1 Rsi/Ti-E (Nikon,
-
DNA binding by BosR controls RpoS-dependent and -independent gene expression in Borrelia burgdorferi
Where the paper cites Pel-Freez
…(100 µg.mL -1 ), kanamycin (100 µg/mL), and/or gentamicin (5 µg/mL). B. burgdorferi strains (Table S6) were cultivated in Barbour–Stoenner–Kelly (BSK)-II medium supplemented with 6% rabbit serum (Pel-Freeze Biologicals, Rogers, AR) and antibiotics when appropriate: kanamycin (400 µg/mL), streptomycin (100 µg.mL -1 ), and gentamicin (50 µg.mL -1 ). B. burgdorferi plasmid contents were verified by PCR as described…
-
Divergent humoral immune signatures in response to Klebsiella pneumoniae bacteremia
Where the paper cites Pel-Freez
…combined with 20 µl of heat-inactivated human serum (inactivated at 56°C for 30 minutes) in 96-well U-bottom plates and incubated shaking at 37°C for 1 h. Subsequently, 10 µl of baby rabbit complement (Pel-Freez Biologics) was added to a final concentration of 10%, and plates were incubated shaking for 1 h at 37°C. All samples were tested in triplicate. Controls included bacteria alone, bacteria with serum only,…
-
Complement-mediated enhancement of SARS-CoV-2 antibody neutralisation potency in vaccinated individuals
Where the paper cites Pel-Freez
…of 1:100 or 1:500, conducted in duplicate. The beads and serum were incubated for 30 min at RT whilst shaking at 700 rpm, then washed twice in 100 µl of wash buffer (PBS containing 0.1% tween-20) and resuspended in 50 µl of HBSS with 10% IgG- and IgM-depleted human complement (Pel-Freeze Biologicals). The samples were incubated for 20 min at 37 °C whilst shaking at 700 rpm, then washed twice in
-
Activation of the Carotid Body by Kappa Opioid Receptors Mitigates Fentanyl-Induced Respiratory Depression
Where the paper cites Pel-Freez
…ry neurons by µ opioid receptors and suggest that KOR agonists might counteract the central depressive effects of opioids on breathing. … The primary antibodies used were rabbit anti-TH antibody (Pel-Freez Biologicals; #P40101-0; dilution 1:1000), mouse anti-TH antibody (Sigma, #T1299, dilution 1:1000), guinea pig anti…
-
Acquired tick resistance in Peromyscus leucopus alters Ixodes scapularis infection
Where the paper cites Pel-Freez
…fed on burden-matched hosts during primary infestations. B. burgdorferi B31 (strain MSK5) was cultured in modified Barbour-Stoenner-Kelly II (BSK-II) medium supplemented with 6% normal rabbit serum (NRS; Pel-Freez; 31126-5) and maintained at 37°C and 5% CO 2 . Spirochete growth was monitored daily using dark-field microscopy. Prior to infection, PCR was used to screen the plasmid profiles of B. burgdorferi…
-
A brain-shuttled antibody targeting alpha synuclein aggregates for the treatment of synucleinopathies
Where the paper cites Pel-Freez
…PBS followed by a blocking process using 1% H 2 O 2 . Immunohistochemical detection was carried out with anti-pSer129-α-Syn antibody (S129) (Abcam, ab51250, 1:500) or anti-tyrosine hydroxylase antibody (Pel-Freez, 40101, 1:1000) using avidin-biotin complex (ABC) system (Vectastain ABC Elite Kit, Vector Laboratories, Burlingame, CA). The immunocomplexes were visualized with the chromogen 3,3′-diaminobenzidine…
-
Sequential recruitment of F-BAR proteins controls cytoskeletal crosstalk at the yeast bud neck
Where the paper cites Pel-Freez
…actin and the proteolytic fragments of myosin J. Biol. Chem. 1971; 246 :4866-4871 Abstract Full Text (PDF) PubMed Google Scholar generated from frozen ground hind leg muscle tissue of young rabbits (Pel-Freez Biologicals, Rogers, AR). Lyophilized acetone powder stored at −80°C was mechanically sheared in a coffee grinder, resuspended in G-buffer (5 mM Tris-HCl, pH 8.0, 0.2 mM ATP, 0.5 mM dithiothreitol [DTT], 0.1…
-
The therapeutic use of clonal neural stem cells in experimental Parkinson´s disease
Where the paper cites Pel-Freez
…owing primary anti bodies were used: TH (1:400; Pel-Freez P40101-150), glial fibrillary acidic protein (GFAP) (1:1000; DAKO Z0334), ionized calcium-binding adapter molecule 1 (Iba1) (1:1000; Wako 019-19741), nestin (NES) (1:300; Novus Biologicals NB100-1604), Ki-67 (1:100; Ther moFisher Scientific RM-9106-S1), doublecortin (DCX) (1:300; Santa Cruz sc-8066), STEM121 (1:500; Takara Bio Y40410),
-
Naturally acquired antibodies against 4 Streptococcus pneumoniae serotypes in Pakistani adults with type 2 diabetes mellitus
Where the paper cites Pel-Freez
…strains of pneumococcal serotype 19F, 18C, and 9V. Plates were incubated for 30 minutes on a shaker (750 RPM) at room temperature, followed by the addition of 10 μL baby rabbit complement (Pel-freeze biologicals) and differentiated HL-60 cells (cells: bacteria was 200:1). Plates were incubated for another 45 minutes at 37°C in a humidified chamber, followed by cooling on ice for 15 minutes to stop…
-
Distinct negative elongation factor conformations regulate RNA polymerase II promoter-proximal pausing
Where the paper cites Pel-Freez
…rus strains Escherichia coli DH5-alpha New England Biolabs C2987 Escherichia coli BL21 (DE3) RIL Merck 230280 Escherichia coli DH10EMBacY Geneva Biotech N/A Biological samples Porcine thymus tissue Pel-Freez Biologicals 59451-2 Chemicals, peptides, and recombinant proteins RNA Polymerase II Vos et al., 201840 N/A SPT4 (DSIF) Bernecky et al., 201762 N/A SPT5 (DSIF) Bernecky et al., 201762 N/A NELF-A (NELF) Vos et…
-
Dipyridamole Ameliorates Memory Impairment and Increases Hippocampal Calbindin Expression in Niemann Pick C1 Mice
Where the paper cites Pel-Freez
RE INFANTE … Frozen rabbit liver (200-300 grams, Pel - Freez Biologicals) was thawed…
-
High-Throughput Luminescence-Based Serum Bactericidal Assay Optimization and Characterization to Assess Human Sera Functionality Against Multiple Shigella flexneri Serotypes
Where the paper cites Pel-Freez
…represents the assay conducted using the new strains. Click here to enlarge figure --> The same samples were then used to assess the impact of the use of a different source of BRC on L-SBA results. When Pel-Freeze BRC was utilized instead of the BRC obtained from Cederlane used in all SBA experiments conducted so far, no significant differences were observed in IC50 measured at visit 1 (baseline) and visit 4…
-
Development and validation of a standardized human complement serum bactericidal activity assay to measure functional antibody responses to Neisseria gonorrhoeae
Where the paper cites Pel-Freez
…strain lacking pora expression and expressing a hybridized porb (och) [18 ]. see table s3 for a complete description of pc reagents. 3.4 intrinsic sba growth assay igg/igm-depleted human complement (c′) pooled serum (pel-freez validation lot #03642) was screened for endogenous killing activity against ng strains f62, ms11, and fa1090 as previously described [19 ] with modifications. briefly, sialylated or non-sial…
-
Three neutralizing mAbs induced by MPXV A29L protein recognizing different epitopes act synergistically against orthopoxvirus
Where the paper cites Pel-Freez
…assay The neutralizing activity of the mAbs and sera was determined using the MV or EV forms of the VACV Tian tan or WR strain. Neutralization of VACV EV was performed using a 10% baby rabbit complement (Pelfreez). For EV neutralization, MV was depleted with blocking mAbs at 20 mg/mL. BHK-21 cells were maintained in DMEM sup- plemented with 10% calf serum at 37°C, and 5% CO2. On the day of the experiment, BHK-21…
-
The Group A Streptococcal Vaccine Candidate VAX-A1 Protects against Group B Streptococcus Infection via Cross-Reactive IgG Targeting Virulence Factor C5a Peptidase
Where the paper cites Pel-Freez
…to the manufacturer’s protocol. Neutrophils were adjusted to a concentration of 5 × 10 6 cells/mL in RPMI 1640 tissue culture media, and the bacteria were pre-incubated with baby rabbit complement (PelFreez, #31061) and heat-inactivated fetal bovine serum (FBS, Cat# 97068-085, VWR International). GBS strains were grown to the mid-logarithmic growth phase of OD 600 = ~0.4 and washed twice with sterile…
-
Plant-Based Dairy Alternatives—A Future Direction to the Milky Way
Where the paper cites Pel-Freez
…Chicken pancreas powder were obtained from Pel-Freez biologicals, USA; while toluene and folic acid were purchased from Sigma-Aldrich, USA. Protease and α-amylase enzymes…
-
Gamma-Irradiated Non-Capsule Group B Streptococcus Promotes T-Cell Dependent Immunity and Provides a Cross-Protective Reaction
Where the paper cites Pel-Freez
…adjusted to 10 5 CFU/mL. Following incubation at room temperature for 30 min with shaking, 40 µL of a differentiated HL-60 cell suspension containing 4 × 10 5 cells and 10 µL baby rabbit complement (BRC; Pel-Freez Biological: Rogers AR) was added. The cell mixture was incubated for 45 min at 37 °C with shaking. Next, 10 µL of the final mixture was spotted onto THY agar containing 1.5% Bacto agar (BD Biosciences).…
-
Catecholaminergic axon innervation and morphology in flat‐mounts of atria and ventricles of mice
Where the paper cites Pel-Freez
…ript Author Manuscript Author Manuscript Author Manuscript Author Manuscript Author Manuscript Author Manuscript Author Manuscript Bizanti et al. Page 43 Table 1: Antibodies used in immunohistochemistry: Antibody Concentration Host Company Catalog number Excitation RRID Anti-TH # 12 μL/mL Rabbit Pel-Freeze P40101 n/a AB_461064 Anti-Rabbit ## 24 μL/mL Donkey Invitrogen A-21207 594 nm AB_2762827
-
Allosteric modulation of ryanodine receptor RyR1 by nucleotide derivatives
Where the paper cites Pel-Freez
…Rabbit muscle tissue (sex unspecified) used for the purification of RyR1 in structural studies was and purchased from PelFreez. Rabbit muscle tissue used for the purification of RyR1…
-
Agglutination and hemolytic crossmatching to determine transfusion reaction differences between large and small breed goats
Where the paper cites Pel-Freez
…followed by transfer of supernatant into a clean glass tube. A 1:8 dilu- tion of the final supernatant in 0.9% sterile saline (Braun, Irvine, California) was prepared. Commercial rabbit complement (Pel-Freez Biologicals, Rogers, AR) then was diluted in the prepared diluted goat serum to create a 1:2 dilution of complement: dilute serum. Aliquots were placed in cryogenic tubes (Thomas Scientific, Swedesboro, New…
-
The Lack of Natural IgM Increases Susceptibility and Impairs Anti-Vi Polysaccharide IgG Responses in a Mouse Model of Typhoid
Where the paper cites Pel-Freez
…Ty2 in DPBS (250–500 CFU) was added to each well of a round-bottom polypropylene 96-well plate containing 50 μl of heat-inactivated serum in serial dilutions, 12.5 μl of baby rabbit complement (Pel-Freez Biologicals, Rogers, AR), and 27.5 μl of DPBS. Triplicate samples of each dilution were incubated for 120 min at 37°C with gentle rocking, and 10 μl of this mixture were plated on LB agar plates for counting…
-
Assessment of temporal changes in thickness and needle insertion force in rabbit corneas during storage
Where the paper cites Pel-Freez
…The rabbit whole eyes (N=5) were purchased from PelFreez and they were harvested from Albino rabbits (4.75-5.75 lbs, approximately 8-12 weeks old, mixed gender) on day 0. The…
-
Anti-Ki/anti-PA28γ autoantibodies contribute to the HEp-2 indirect immunofluorescence nuclear speckled pattern
Where the paper cites Pel-Freez
…A commercial preparation of lyophilized rabbit thymus extract (Pel-Freeze Biologicals, Rogers, Arkansas) was used as the source of saline-extractable nuclear antigens. Positive…
-
DA-9805 protects dopaminergic neurons from endoplasmic reticulum stress and inflammation