Gingerich et al. (2021). Infection and Immunity. doi: 10.1128/iai.00747-20
…were mixed with HBSS as a negative control, and purified human serum IgG was used as a positive control. Differentiated HL-60 cells were washed twice with HBSS and mixed with baby rabbit complement (Pel-Freez Biologicals) at a final concentration of 10% in each well. Following this, 60ml (1 106 viable cells) of differentiated HL60 cells and complement were added to the mixture of bacteria and antibodies and…
Pel-Freez products cited: Baby Rabbit Complement (31061)
Abstract
Streptococcus pneumoniae remains a leading cause of bacterial pneumonia despite the widespread use of vaccines. While vaccines are effective at reducing the incidence of most serotypes included in vaccines, a rise in infection due to nonvaccine serotypes and moderate efficacy against some vaccine serotypes have contributed to high disease incidence.