Publications
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Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing
Where the paper cites Pel-Freez
…at 100 °C. Proteins were separated with 12% SDS–PAGE and blotted onto a membrane. Blotted membranes were incubated overnight at 4 °C, with an anti-tyrosine hydroxylase antibody (1:1000; P60101-150, Pel-Freez Biologicals). Antibody-reactive bands were then visualized using an enhanced chemiluminscence (ECL) kit (DG-WF200; Dogen). The experimenter was not blinded to the treatment. None of the cell cultures were…
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Neisseria meningitidis urethritis outbreak isolates express a novel factor H binding protein variant that is a potential target of group B-directed meningococcal (MenB) …
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Development of Complement Factor H-Based Immunotherapeutic Molecules in Tobacco Plants Against Multidrug-Resistant Neisseria
Where the paper cites Pel-Freez
…carcinoembryonic Ag–related cell adhesion molecule 3 (CEACAM3) that is expressed by PMNs and results in phagocytosis ( 42 ). FH*/Fc was added at different concentrations, followed by 10% human complement (Pel-Freez). The reaction mixtures were incubated for 60 min at 37°C in a shaking water bath. Bacteria were serially diluted and plated at 0 and 60 min on chocolate agar plates. Percentage survival of gonococci in…
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An Opsonophagocytic Killing Assay for the Evaluation of Group A Streptococcus Vaccine Antisera
From the abstract
Group A Streptococcus (GAS) is a major cause of global mortality, yet there are no licensed GAS vaccines. Vaccine progress has been hampered, in part, by a lack of standardized assays able to quantify antibody function in test antisera. The most widely used assay was developed over 50 years ago by Rebecca Lancefield and relies on human whole blood as...
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Non-invasive in vivo imaging of acute thrombosis: development of a novel factor XIIIa radiotracer
Where the paper cites Pel-Freez
…vein of healthy volunteers through a series of three cylindrical perfusion chambers maintained at 37C in a water bath. Each chamber contained a carefully prepared strip of porcine aorta (Pel-Freez Biologicals, CA, USA) from which the intima and a thin layer of media had been removed. Rheological conditions in the first chamber simulated those of patent medium-sized coronary arteries (low-shear rate, 212…
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Enhancing Rose Bengal-Photosensitized Protein Crosslinking in the Cornea
Where the paper cites Pel-Freez
…4.0 International License. Downloaded from iovs.arvojournals.org on 05/05/2019 METHODS Tissue Preparation Mature New Zealand White freshly frozen rabbit eyes (Pel- Freez Biologicals, Rogers, AR, USA) were defrosted in a moist chamber. The epithelium was removed using a hockey knife (Katena, Denville, NJ, USA), and the surface was polished with a small swab. The nasal-temporal…
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Visualization of spirochetes by labeling membrane proteins with fluorescent biarsenical dyes
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Tuning shape and internal structure of protein droplets via biopolymer filaments
Where the paper cites Pel-Freez
…esults demonstrate a versatile approach to construct tunable, anisotropic macromolecular liquids. … Monomeric actin (G-actin) is purified from rabbit skeletal muscle acetone powder (Pel Freeze Biologicals, Product code: 41008-3) using a procedure adapted from ref. 27 and stored in 2…
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The maximum possible amount of drug in rapidly separating microneedles
Where the paper cites Pel-Freez
…USA). PVA (75% hydrolyzed, MW6000 Da) and polylactic acid (PLA) (3051D) were purchased from Acros Organics, Geel, Belgium. Sulforhodamine B was procured from (Molecular Probes, Eugene, OR). Sucrose was purchased from Sigma-Aldrich, St. Louis, MO. Procine cadaver skin was purchased from Pel-Freez, Rogers, AR. All reagents were prepared in ultrapure water. All chemicals used were of high purity g
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Preclinical efficacy of a lipooligosaccharide peptide mimic candidate gonococcal vaccine
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MALDI imaging directed laser ablation tissue microsampling for data independent acquisition proteomics
Where the paper cites Pel-Freez
…were achieved using UPLC‐HDMS- E. The list of identified proteins was used for identification of MALDI MSI peaks. 2 | EXPERIMENTAL 2.1 | Sample preparation Fresh rat brain tissue samples (Pel‐Freez Biologicals, Rogers, AR) were sectioned at −20°C using a cryostat (CM 1850, Leica Microsystem, Wetzlar, Germany). Optimal cutting temperature solution (OCT, Sakura Finetek, Torrance, CA) was used to fix one…
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Diagnosis of cyanide poisoning using an automated, field-portable sensor for rapid analysis of blood cyanide concentrations
Where the paper cites Pel-Freez
…(10 mM NaOH) to ensure CN remained as non-volatile CN-. Sample collection, storage and preparation For analytical method development, rabbit whole blood, with EDTA as anti-coagulant, was purchased from Pel-Freez (Arkansas, USA) and stored at -80 °C until analysis. Additionally, New Zealand White rabbits (3.5-4.5 kg) were exposed to CN at the University of California, Irvine (UCI), and whole blood was drawn at…
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Contact Lens Based Drug Delivery to the Posterior Segment Via Iontophoresis in Cadaver Rabbit Eyes
Where the paper cites Pel-Freez
…caustic species. Our results support the possibility for the delivery of therapeutics to the posterior eye segment through iontophoresis in significant quantities. METHODS Materials Mature cadaver albino rabbit eyes were purchased from Pel- Freez Biologicals (Rogers, AR). The nile blue A dye (75% dye content, MW: 354 g/mol, net charge: +1) and fluorescein (MW: 332 g/mol, net charge: −1) were pu
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Complement-Dependent Serum Bactericidal Assays for Neisseria gonorrhoeae
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Collagenase injection into the suprachoroidal space of the eye to expand drug delivery coverage and increase posterior drug targeting
Where the paper cites Pel-Freez
…by dynamic light scattering (Zetasizer Nano ZS, Malvern Panalytical, Westborough, MA) to identify possible particle aggregation. 2.2 Ex vivo injection procedure Prior to SCS injection, frozen rabbit eyes (Pel-Freez Biological, Rogers, AR) were thawed at room temperature for 30 min, after which most fat and conjunctiva were removed to expose a clear injection spot and facilitate analysis of the specimen. To adjust…
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Analysis of TRPA1 antagonist, A-967079, in plasma using high-performance liquid chromatography tandem mass-spectrometry
Where the paper cites Pel-Freez
…O A-967079 AP18 HC-030031 Analysis of TRPA1 antagonist, A-967079 6 2.2. Biological Samples For analytical method development and validation, rabbit plasma (non-sterile with EDTA) was purchased from Pel-Freeze Biologicals and was stored at -80 °C until used. Rabbit plasma was used to develop the method presented here because we planned to utilize a rabbit model developed by our collaborators to prove the…
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Analysis of potential cyanide antidote, dimethyl trisulfide, in whole blood by dynamic headspace gas chromatography-mass spectroscopy
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Ponceau S waste: Ponceau S staining for total protein normalization
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Age-stratified analysis of serotype-specific baseline immunity against group B streptococcus
Where the paper cites Pel-Freez
…plates. After 30 min of Accepted Manuscript 7 incubation at room temperature, 42.8 μl of a differentiated HL-60 cell suspension (4.28 × 105 cells per well) and 7.2 μl of baby rabbit complement (BRC; Pel-Freez Biological; Rogers, AR) were added to each well, after which the mixture was incubated for 45 min at 37 °C with shaking. Afterwards, 10 µL of the final reaction mixture from each well was spotted onto…
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Neuroprotection and immunomodulation of progesterone in the gut of a mouse model of Parkinson's disease
Where the paper cites Pel-Freez
…blue solution to colour myenteric ganglia neu‐ rones.47 Dopaminergic neurones were detected by an overnight incu‐ bation with a polyclonal primary antibody for TH (rabbit anti‐tyrosine hydroxylase; Pel Freez, Rogers, AR, USA; catalogue no. P40101‐150; dilution 1:1000) followed by incubation with a biotinylated goat anti‐ rabbit secondary antibody (Vector Laboratories, Inc., Burlingame, CA, USA; catalogue no.…
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The influence of hydration on different mechanical moduli of the cornea
Where the paper cites Pel-Freez
…microscopy) and to see whether E and M are changing correspondingly. Materials and methods Sample preparation Twenty-seven adult (12 to 24 months of age) New Zealand White frozen rabbit eyes (Pel-Freez Biologicals, Roger, AR, USA) were thawed in air. The epithelium was removed using a blunt hockey knife. According to the location of the muscle insertions and the optical nerve, the vertical axis of the cornea…
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The oligopeptide ABC transporter OppA4 negatively regulates the virulence factor OspC manufacturing of the Lyme disease pathogen
Where the paper cites Pel-Freez
…abata (Kawabata et al., 2004). B. burgdorferi were cultivated in Barbour-Stoenner-Kelly (BSK-II) medium supplemented with 6% normal rabbit serum (Pel-Freez Biologicals, Rogers, AR) at 37°C with 5% CO2. Relevant antibiotics were added to the cultures at the following final concentrations: 300 µg/ml for kanamycin (Kan), 50 µg/ml for streptomycin (Strep), and 50 µg/ml for gentamicin (Gent). The co
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Small-molecule BCL6 inhibitor effectively treats mice with nonsclerodermatous chronic graft-versus-host disease
Where the paper cites Pel-Freez
…monoclonal antibody followed by rabbit complement (Pel-Freez … coated overnight with 1 µg anti-mouse Ig (Rockland) per well, … antibody followed by rabbit complement (Pel-Freez…
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Short-chain fatty acids alter metabolic and virulence attributes of Borrelia burgdorferi
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Rational design and evaluation of an artificial Escherichia coli K1 protein vaccine candidate based on the structure of OmpA
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Purification of globular actin from rabbit muscle and pyrene fluorescent assays to investigate actin dynamics in vitro
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Proteomic Analysis of S-Palmitoylated Proteins in Ocular Lens Reveals Palmitoylation of AQP5 and MP20
Where the paper cites Pel-Freez
…enrich for palmitoylated proteins. Finally, the biotin groups are removed and sulfhydryl groups are alkylated by iodoacetamide. Specific experimental details for our study are as follows: a frozen 1-year-old bovine lens (Pel-Freez Biologicals, Rogers, AK, USA) was decapsulated and the outer 2-mm thick tissue was isolated from the surface of the lens. Tissue was homogenized in homogenizing buffer
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New SNP Markers to Identify Culex Complex Species (Diptera: Culicidae) by High-Resolution DNA Melting Analysis
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Multiple TOF/TOF events in a single laser shot for multiplexed lipid identifications in MALDI imaging mass spectrometry
Where the paper cites Pel-Freez
…erica, MA). Rat brain was purchased from Pel-Freez Biologicals (Rogers, AR). Transverse sections of flash-frozen rat brain tissue were cut at 10 m thickness using a cryostat (CryoStar NX70 Cryostat (Thermo Scientific, Waltham, MA) and thaw mounted onto indium tin oxide (ITO) coated glass slides. A custom-built sublimation apparatus was used to apply a DHB matrix layer (120ºC at <70 mTorr for 7
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Ionotropin is the mammalian digoxin-like material (DLM). It is a phosphocholine ester of a steroid with 23 carbon atoms
Where the paper cites Pel-Freez
…solvent and a new sample was injected with the auto-injector. Data is presented as generated by the software. Source of tissues and blood: The following tissues and blood samples were purchased from Pel Freez Biologicals (Pel Freez Biologicals LLC, 219 N. Arkansas Street, Rogers, AR 72756): [a] horse serum (Equus ferus caballus), [b] pig adrenals (Sus scrofa domesticus), [c] cattle adrenals (Bos taurus), [d] sheep…