Ozdilek et al. (2021). mBio. doi: 10.1128/mbio.00800-21
…and 1% L-glutamine. HL-60 cells were differentiated using 0.6% N,N-dimethylformamide (DMF) for 3 days before performing the OPKA assay, harvested, and resuspended in OBB. Baby rabbit complement (Pel- Freez) was added to HL-60 cells at a 1:5 final volume. The HL-60-complement mixture was added to the bacteria at 5 105 cells/well. The final reaction mixtures were incubated at 37°C for 1 h with shaking. The…
Pel-Freez products cited: Baby Rabbit Complement (31061)
Abstract
Infectious diseases caused by pathogenic bacteria are a major threat to human health. Capsular polysaccharides (CPSs) of many pathogenic bacteria have been used as the main components of glycoconjugate vaccines against bacterial diseases in clinical practice worldwide, with various degrees of success.