Babb et al. (2021). Microbiology Spectrum. doi: 10.1128/spectrum.01446-21
…were diluted 3-fold from 20 mg/ml. ST3 (A66) killing (%) was determined in the presence of humAbs under the following two conditions: with HL60 cells and complement (3- to 4-week rabbit complement; Pel-Freez) or without HL60 cells (humAbs and bacteria only) by plating aliquots of samples onto blood agar plates and enumerating CFU. In vitro bacterial gene expression by reverse transcription-quantitative PCR…
Pel-Freez products cited: Baby Rabbit Complement (31062)
Abstract
Despite the global success of vaccination with pneumococcal conjugate vaccines, serotype 3 (ST3) pneumococcus remains a leading cause of morbidity and mortality. In comparison to other vaccine-included serotypes, the ST3 pneumococcal capsular polysaccharide (PPS3) induces a weaker opsonophagocytic response, which is considered a correlate of vaccine efficacy.