Publications
-
Three-Dimensional Cell Culture Environment Promotes Partial Recovery of the Native Corneal Keratocyte Phenotype from a Subcultured Population
Where the paper cites Pel-Freez
…twice, and stored in milli-Q. Corneal Cell Isolation Rabbit corneal keratocytes were isolated from rabbit corneas using a previous described protocol.13 Corneas were dissected out of fresh rabbit eyes (Pel-Freeze, Rogers, AK). Following dissection, the concave side of the cornea was incubated in PBS plus typsin from porcine Page 6 of 30 Tissue Engineering Part A Three Dimensional Cell Culture Environment Promotes…