Publications

1 publications

  1. 2016Abraham et al. (2016). Arthritis & Rheumatology.

    Brief Report: Anti-Eukaryotic Initiation Factor 2B Autoantibodies Are Associated With Interstitial Lung Disease in Patients With Systemic Sclerosis

    Where the paper cites Pel-Freez

    …were screened at 1:40 and where anti- eIF2B positive, titred by serial dilution. Counter Immune Electrophoresis CIEP was performed as described previously (7) using lyophilised rabbit thymus extract (Pel- Freez, USA) and bovine spleen extract in PBS (Sigma). Protein Immunoprecipitation (IPP) using [35S]-methionine 10 µl sera was mixed with 2 mg protein-A-Sepharose beads (Sigma, UK) in IPP buffer (10 mM Tris-Cl…