Scholz, M. et al. Tuning molecular motor transport through cytoskeletal filament network organization. Soft Matter 16, 2135-2140 (2020).
…in terms of the known features of the crosslinking proteins. Materials and methods Protein and vesicle preparation Monomeric actin was purified from rabbit skeletal muscle acetone powder (Pel-Freez Biologicals) using a protocol adapted from ref. 30. For imaging with fluorescence microscopy, actin monomers were labeled with the fluorophore tetramethyl- rhodamine-6-maleimide (TMR; Life Technologies). Both…
Abstract
Myosin II motor dynamics have signatures that report on the structure of the underlying network of crosslinked cytoskeletal filaments.