Alonso et al. (2023). Journal of Veterinary Internal Medicine. doi: 10.1111/jvim.16738
…followed by transfer of supernatant into a clean glass tube. A 1:8 dilu- tion of the final supernatant in 0.9% sterile saline (Braun, Irvine, California) was prepared. Commercial rabbit complement (Pel-Freez Biologicals, Rogers, AR) then was diluted in the prepared diluted goat serum to create a 1:2 dilution of complement: dilute serum. Aliquots were placed in cryogenic tubes (Thomas Scientific, Swedesboro, New…
Pel-Freez products cited: Baby Rabbit Complement (31062)
Abstract
Abstract Background Blood transfusions are performed frequently in goats, but crossmatches are rarely performed. Hypothesis/Objectives Determine differences in the frequency of agglutination and hemolytic crossmatch reactions between large and small breed goats. Animals Healthy adult goats, 10 large and 10 small breed. Methods Two hundred eighty major and minor agglutination and hemolytic crossmatches: 90 large breed donor to large breed recipient (L-L), 90 small breed donor to small breed recipient (S-S), 100 large breed donor to small breed recipient (L-S). A linear mixed model with treatment group (L-L, S-S, L-S) as a fixed effect and individual crossmatch as a random effect was used to identify variations in reaction frequency among groups and individuals. Results Frequency of major agglutination reactions for L-L, S-S, and L-S were 3/90 (3.3%), 7/90 (7.8%), and 10/100 (10.0%), respectively. Frequency of major hemolytic reactions for L-L, S-S, and L-S were 27/84 (32.1%), 7/72 (9.7%), and 31/71 (43.7%). Individual pairings and groupings had no effect on agglutination reactions. Individual pairings had no effect on the frequency of hemolytic reactions. For major hemolytic crossmatches, pairwise comparisons identified higher frequencies of reactions when comparing L-L to S-S (P = .007) and L-S to S-S (P < .001). Conclusion and Clinical Importance Goats experience increased frequencies of hemolytic reactions compared to agglutination. Significant increases in hemolysis were seen between large breed donors and small breed recipients, compared to small breed pairings. Additional studies are required to determine correlations between crossmatches and transfusion reactions.