Rabbit Muscle Acetone Powder

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1. 2021Lakha et al. (2021). Biochemistry.

   Variable Autoinhibition among Deafness-Associated Variants of Diaphanous 1 (DIAPH1)

   Where the paper cites Pel-Freez

   …To isolate actin, 3 g of rabbit muscle acetone powder (Pel Freez Biologicals) was mixed with 100 mL of actin extraction buffer (2 mM Tris-HCl, 0.2 mM CaCl 2 , 0.2 mM ATP, and 1 mM…

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2. 2020Shaaban et al. (2020). Nature Structural & Molecular Biology.

   Cryo-EM reveals the transition of Arp2/3 complex from inactive to nucleation-competent state

   Where the paper cites Pel-Freez

   …Nature Structural & Molecular BioloGy | www.nature.com/nsmb Articles Nature Structural & Molecular Biology Methods Protein purification. Rabbit skeletal muscle actin was extracted from acetone powder (Pel Freeze, catalog no. 41995) by stirring in G-buffer (20 ml g–1, 2 mM Tris pH 8.0, 0.2 mM ATP, 0.5 mM DTT, 0.1 mM CaCl2) on ice for 30 min before spinning in a Beckman JA-20 rotor at 16,000 r.p.m. for 30 min at 4…

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3. 2015Orlova et al. (2015). Biochemical Journal.

   Actin polymerization is stimulated by actin cross-linking protein palladin

   Where the paper cites Pel-Freez

   …Purified protein was stored in HEPES buffer at 4 ◦C (20 mM HEPES, pH 7.5, 5 mM dithiothreitol (DTT) and 50 mM NaCl) and used within 2–4 weeks. Actin was purified from rabbit muscle acetone powder (Pel- Freez Biologicals) by using the method of Spudich and Watt [21] and gel-filtered on 16/60 SephacrylTM S-200 column (GE Healthcare Life Sciences). Purified monomeric actin was stored at 4 ◦C in G-buffer (5 mM Tris/HCl,…

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• Fascin-induced actin protrusions are suppressed by dendritic networks in GUVs.

  Wubshet, N. H., Bashirzadeh, Y. & Liu, A. P. Fascin-induced actin protrusions are suppressed by dendritic networks in GUVs. Mol. Biol. Cell mbc.E21-02-0080 (2021).

• Purification of globular actin from rabbit muscle and pyrene fluorescent assays to investigate actin dynamics in vitro

  Sun, H., Luo, Y. & Miao, Y. Purification of Globular Actin from Rabbit Muscle and Pyrene Fluorescent Assays to Investigate Actin Dynamics in vitro. BIO-PROTOCOL 8, (2018).

• Actin polymerization is stimulated by actin cross-linking protein palladin

  Gurung, R. et al. Actin polymerization is stimulated by actin cross-linking protein palladin. Biochem. J. 473, 383–396 (2016).

• Actin bundle architecture and mechanics regulate myosin II force generation

  Weirich, K. L., Stam, S., Munro, E. & Gardel, M. L. Actin bundle architecture and mechanics regulate myosin II force generation. bioRxiv 2020.07.17.207415 (2020).

• Tuning molecular motor transport through cytoskeletal filament network organization

  Scholz, M. et al. Tuning molecular motor transport through cytoskeletal filament network organization. Soft Matter 16, 2135–2140 (2020).

• Twinfilin bypasses assembly conditions and actin filament aging to drive barbed end depolymerization

  Shekhar, S., Hoeprich, G. J., Gelles, J. & Goode, B. L. Twinfilin bypasses assembly conditions and actin filament aging to drive barbed end depolymerization. J. Cell Biol. 220, (2021).

• Confinement Geometry Tunes Fascin-Actin Bundle Structures and Consequently the Shape of a Lipid Bilayer Vesicle

  Bashirzadeh, Y., Wubshet, N. H. & Liu, A. P. Confinement Geometry Tunes Fascin-Actin Bundle Structures and Consequently the Shape of a Lipid Bilayer Vesicle. Front. Mol. Biosci. 7, (2020).