Human Serum, Single Donor, Heat Inactivated, IgG/IgM/IgA Depleted
Human serum from individual donor units is depleted of IgG, IgM, and IgA antibodies, 0.2 µm filtered, aliquoted, heat-inactivated, and frozen.
Specifications
Physical State:
Frozen Liquid
Storage:
-70°C or below
Release criteria
Complement activity (CH50):
Negative
Residual IgG:
< 10%
Residual IgM:
< 10%
Residual IgA:
< 10%
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Species: | Human |
|---|---|
| Product Types: | Serum |
| Source: | OTC Serum |
| Pooling: | Single Donor |
| Donor Sex: | Mixed |
| Depletion Process: | IgA Depleted, IgG Depleted, IgM Depleted |
| Processing Method: | Heat Inactivated |
| Physical State: | Frozen Liquid |
| Application Notes: | Suitable for sample dilution, immunoassays, control serum, and various applications requiring serum supplementation. |
| Testing: | CH50: Negative Residual IgG: <10% Residual IgM: <10% Residual IgA: <10% Serum tested negative for infectious disease per FDA guidelines. |
| Storage Conditions: | -70°C or below |
| Shipping Conditions: | Dry ice |
| Packaging: | Cryovial, Poly bottle |
| Expiration: | 1 year from date of receipt when stored continuously at -70°C or below; avoid repeated freeze/thaw. Product quality is guaranteed to meet Pel-Freez Biologicals' specifications for this period when stored as indicated. |
Product Applications
- Serum bactericidal assay
- Opsonophagocytic assay
- Complement-dependent cytotoxicity
- Hemolytic complement assay
- Assay diluent & negative matrix
- Assay controls & calibrators
- Toxicology assays
- Vaccine development
- In vitro diagnostics
Documents & Datasheets
Certificate of Analysis
Frequently Asked Questions
How should I thaw frozen serum or plasma?
Put the bottle in a refrigerator at 2-8°C overnight, or in a 37°C water bath with the water kept below the cap, and swirl it gently as it thaws. Take it out as soon as the last ice has melted; never thaw above 37°C, on the bench or in a microwave. Then mix, aliquot what you will not use right away and return the aliquots to the label temperature. The full walkthrough with diagrams: how to thaw frozen serum.
Can I refreeze serum after it has thawed?
Aliquots can go back to the freezer once; the bulk bottle should not. Every freeze-thaw cycle concentrates salts, shifts pH and stresses the proteins, and complement activity drops with each one. Split the bottle into single-use tubes on the first thaw so each portion is only ever thawed once, or hold the thawed bottle at 2-8°C and use it within the window on the product page. See how to thaw frozen serum.
My thawed serum is cloudy or has particles in it. Is it still good?
Usually yes. Some haze or flocculent material after thawing is cryoprecipitate (fibrin, lipoprotein and calcium salts that came out of solution while frozen), and it does not change how the serum performs. Warm the bottle briefly to 37°C and swirl to redissolve most of it, or filter through a 0.45 µm filter if you need a clear product. A strong odor, a color change, or turbidity that keeps increasing at room temperature is contamination, not cryoprecipitate: do not use the bottle; contact us with the lot number. More in how to thaw frozen serum.
What is complement activity (CH50 / hemolytic titer) and how is it tested?
The hemolytic titer measures how well complement lyses antibody-sensitized sheep red blood cells; CH50 is the dilution of serum that lyses 50% of the cells. It is the release test for every lot of our complement products, and our heat-inactivated human products are CH50 tested to confirm that complement activity is absent.
How is Pel-Freez Biologicals' antibody-depleted complement made?
We have developed a proprietary process for antibody removal based on affinity chromatography. It removes the immunoglobulins while maintaining high complement potency; the residual-antibody specification is given in the next question.
What's the difference between antibody-depleted human complement (ADHC), heat-inactivated ADHC (ADHC-HI) and antibody-depleted human serum (ADHS)?
- ADHC (antibody-depleted human complement) keeps high complement potency for assays that need functional complement.
- ADHC-HI is the same material heat-inactivated and CH50 tested to confirm no complement activity, for applications that need the other serum components but not complement.
- ADHS (antibody-depleted human serum) starts from normal human serum that was not collected to preserve complement; it is neither heat-inactivated nor CH50 tested, and serves as a negative matrix for dilution, QC samples, or cell culture where complement would inhibit growth.
Show 5 more questions
Which immunoglobulins are removed from your antibody-depleted human products?
We offer IgG-depleted, IgG/IgM-depleted and IgG/IgM/IgA-depleted versions of both our human complement and our human serum, all made by the same affinity chromatography process. Match the depletion to the isotypes your assay detects.
Are there residual antibodies in Pel-Freez Biologicals' antibody-depleted complement?
Our specification for residual antibody in antibody-depleted products is <3.5% of the feed serum for each isotype. Typical residual IgG is <2% and IgM is usually undetectable after depletion. Because assay conditions vary widely, many customers sample one or more lots to confirm they suit their specific system.
How is antibody-depleted human serum used as an assay control or matrix?
With the immunoglobulins removed, antibody-depleted serum is a low-background matrix for diluting samples, preparing QC and calibrator samples, and for add-back experiments where the removed isotype is spiked back in to confirm recovery.
What is the difference between pooled and single-donor products?
Pooled products combine material from multiple donors, which averages out donor-to-donor variation and gives large, uniform lots. Single-donor products come from one donor: their properties trace to that donor and can differ more between lots. Studies that need a single, well-characterized source, such as one complement lot carried through a clinical trial, use single-donor material.
What is heat-inactivated serum and when should I use it?
Heat-inactivated serum has been held at 56°C for 30 minutes to destroy complement activity. Use it when you need the other serum components without functional complement, for example as a cell culture supplement or an assay matrix where active complement could lyse cells or cause non-specific killing.
