Human Serum, Pooled, Heat Inactivated, IgG/IgM Depleted
Pooled human serum depleted of IgG and IgM, heat-inactivated and frozen.
Specifications
Physical State:
Frozen Liquid
Storage:
-70°C or below
Release criteria
Complement activity (CH50):
Negative – Not detected
Residual IgG (HPLC):
< 3.5%
Residual IgM (HPLC):
< 3.5%
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Species: | Human |
|---|---|
| Product Types: | Serum |
| Source: | OTC Serum |
| Pooling: | Pooled |
| Donor Sex: | Mixed |
| Depletion Process: | IgG Depleted, IgM Depleted |
| Processing Method: | Heat Inactivated |
| Physical State: | Frozen Liquid |
| Application Notes: | Suitable as a negative matrix diluent. |
| Testing: | CH50: Negative Residual IgG: <3.5% Residual IgM: <3.5% Each donor unit was tested prior to pooling according to FDA guidelines for the detection of Hepatitis B Surface Antigen, Antibodies to HIV and HVC, HIV-1 RNA, HBV DNA, HCV RNA, WNV RNA, and Syphilis. Each donor has been tested according to FDA guidelines for T. Cruzi (Chagas). All units yielded NON-REACTIVE/NEGATIVE results for each test performed. All blood is collected in the United States of America from human donors in FDA licensed centers and tested with FDA approved test kits. No test method can provide total assurance that Hepatitis B Virus, Hepatitis C Virus, Human Immunodeficiency Virus or other infectious agents are absent. |
| Storage Conditions: | -70°C or below |
| Shipping Conditions: | Dry ice |
| Packaging: | Cryovial, Poly bottle |
| Expiration: | 1 year from date of receipt when stored continuously at -70°C or below; avoid repeated freeze/thaw. Product quality is guaranteed to meet Pel-Freez Biologicals' specifications for this period when stored as indicated. |
Product Applications
- Serum bactericidal assay
- Opsonophagocytic assay
- Complement-dependent cytotoxicity
- Hemolytic complement assay
- Assay diluent & negative matrix
- Assay controls & calibrators
- Toxicology assays
- Vaccine development
- In vitro diagnostics
Documents & Datasheets
Datasheet
- 34020 Human Serum IgG-IgM Depleted Pooled Serum Heat-Inactivated (Rev 1)
- ADHS Product Information Sheet
Safety Datasheet
Certificate of Analysis
| Lot | Issued | |
|---|---|---|
| 34746B | Jan 8, 2025 | Download |
| 34746A | Jan 8, 2025 | Download |
| 31846 | Jan 8, 2025 | Download |
| 19046A | Jul 16, 2024 | Download |
| 19046B | Jul 16, 2024 | Download |
| 11646 | May 7, 2024 | Download |
| 26444 | Oct 27, 2022 | Download |
| 11744 | May 26, 2022 | Download |
| 13844 | May 26, 2022 | Download |
| 09644 | Apr 25, 2022 | Download |
Frequently Asked Questions
What is complement activity (CH50 / hemolytic titer) and how is it tested?
The hemolytic titer measures how well complement lyses antibody-sensitized sheep red blood cells; CH50 is the dilution of serum that lyses 50% of the cells. It is the release test for every lot of our complement products, and our heat-inactivated human products are CH50 tested to confirm that complement activity is absent.
How is Pel-Freez Biologicals' antibody-depleted complement made?
We have developed a proprietary process for antibody removal based on affinity chromatography. It removes the immunoglobulins while maintaining high complement potency; the residual-antibody specification is given in the next question.
What's the difference between antibody-depleted human complement (ADHC), heat-inactivated ADHC (ADHC-HI) and antibody-depleted human serum (ADHS)?
- ADHC (antibody-depleted human complement) keeps high complement potency for assays that need functional complement.
- ADHC-HI is the same material heat-inactivated and CH50 tested to confirm no complement activity, for applications that need the other serum components but not complement.
- ADHS (antibody-depleted human serum) starts from normal human serum that was not collected to preserve complement; it is neither heat-inactivated nor CH50 tested, and serves as a negative matrix for dilution, QC samples, or cell culture where complement would inhibit growth.
Which immunoglobulins are removed from your antibody-depleted human products?
We offer IgG-depleted, IgG/IgM-depleted and IgG/IgM/IgA-depleted versions of both our human complement and our human serum, all made by the same affinity chromatography process. Match the depletion to the isotypes your assay detects.
Are there residual antibodies in Pel-Freez Biologicals' antibody-depleted complement?
Our specification for residual antibody in antibody-depleted products is <3.5% of the feed serum for each isotype. Typical residual IgG is <2% and IgM is usually undetectable after depletion. Because assay conditions vary widely, many customers sample one or more lots to confirm they suit their specific system.
How is antibody-depleted human serum used as an assay control or matrix?
With the immunoglobulins removed, antibody-depleted serum is a low-background matrix for diluting samples, preparing QC and calibrator samples, and for add-back experiments where the removed isotype is spiked back in to confirm recovery.
Show 1 more question
What is heat-inactivated serum and when should I use it?
Heat-inactivated serum has been held at 56°C for 30 minutes to destroy complement activity. Use it when you need the other serum components without functional complement, for example as a cell culture supplement or an assay matrix where active complement could lyse cells or cause non-specific killing.
Publications citing this product (1)
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An mRNA–Lipid Nanoparticle Platform Encoding the Conserved Outer Membrane Protein BamA Elicits Broadly Cross-Reactive Systemic and Mucosal Antibodies Against Antimicrobial-Resistant Neisseria gonorrhoeae
Where the paper cites Pel-Freez
…362 Ng WHO X (1×10³ CFU in 40 µL HBSS) were added to wells containing diluted test sera 363 and incubated for 15 min at 37°C. Subsequently, 10 µL of either IgG/IgM-depleted normal human serum (NHS; Pel-Freeze Biologicals; Cat. # 34010-10, Lot 34943) or heat- inactivated NHS (HI-NHS; Cat. #34020-10, Lot 13844) was added as a 10% (v/v) complement source, and reactions were incubated for 1 h at 37°C. A single lot of…
