Human Serum, Single Donor, IgG/IgM/IgA Depleted, Lyophilized
Human serum from individual donor units is depleted of IgG, IgM, and IgA antibodies, 0.2 µm filtered, aliquoted, frozen, and lyophilized.
Specifications
Physical State:
Lyophilized Powder
Storage:
-20°C or below
Release criteria
Residual IgG:
< 10%
Residual IgM:
< 10%
Residual IgA:
< 10%
Moisture:
< 5%
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Species: | Human |
|---|---|
| Product Types: | Serum |
| Source: | OTC Serum |
| Pooling: | Single Donor |
| Donor Sex: | Mixed |
| Depletion Process: | IgA Depleted, IgG Depleted, IgM Depleted |
| Physical State: | Lyophilized Powder |
| Application Notes: | Suitable for sample dilution, immunoassays, control serum, and various applications requiring serum supplementation. |
| Testing: | Residual IgG: <10% Residual IgM: <10% Residual IgA: <10% Moisture: <5% Serum tested negative for infectious disease per FDA guidelines. |
| Storage Conditions: | -20°C or below |
| Shipping Conditions: | Cool packs |
| Packaging: | Glass vial |
| Expiration: | 1 year from date of receipt when stored dry at -20°C or below; reconstitute just before use and freeze any remainder at -70°C or below. Product quality is guaranteed to meet Pel-Freez Biologicals' specifications for this period when stored as indicated. |
Product Applications
- Serum bactericidal assay
- Opsonophagocytic assay
- Complement-dependent cytotoxicity
- Hemolytic complement assay
- Assay diluent & negative matrix
- Assay controls & calibrators
- Toxicology assays
- Vaccine development
- In vitro diagnostics
Documents & Datasheets
Certificate of Analysis
| Lot | Issued | |
|---|---|---|
| 02948-2898 | Feb 24, 2026 | Download |
| 03048-6466 | Feb 24, 2026 | Download |
| 02948-4149 | Feb 24, 2026 | Download |
| 03048-6446 | Feb 24, 2026 | Download |
Frequently Asked Questions
How is Pel-Freez Biologicals' antibody-depleted complement made?
We have developed a proprietary process for antibody removal based on affinity chromatography. It removes the immunoglobulins while maintaining high complement potency; the residual-antibody specification is given in the next question.
What's the difference between antibody-depleted human complement (ADHC), heat-inactivated ADHC (ADHC-HI) and antibody-depleted human serum (ADHS)?
- ADHC (antibody-depleted human complement) keeps high complement potency for assays that need functional complement.
- ADHC-HI is the same material heat-inactivated and CH50 tested to confirm no complement activity, for applications that need the other serum components but not complement.
- ADHS (antibody-depleted human serum) starts from normal human serum that was not collected to preserve complement; it is neither heat-inactivated nor CH50 tested, and serves as a negative matrix for dilution, QC samples, or cell culture where complement would inhibit growth.
Which immunoglobulins are removed from your antibody-depleted human products?
We offer IgG-depleted, IgG/IgM-depleted and IgG/IgM/IgA-depleted versions of both our human complement and our human serum, all made by the same affinity chromatography process. Match the depletion to the isotypes your assay detects.
Are there residual antibodies in Pel-Freez Biologicals' antibody-depleted complement?
Our specification for residual antibody in antibody-depleted products is <3.5% of the feed serum for each isotype. Typical residual IgG is <2% and IgM is usually undetectable after depletion. Because assay conditions vary widely, many customers sample one or more lots to confirm they suit their specific system.
How is antibody-depleted human serum used as an assay control or matrix?
With the immunoglobulins removed, antibody-depleted serum is a low-background matrix for diluting samples, preparing QC and calibrator samples, and for add-back experiments where the removed isotype is spiked back in to confirm recovery.
What is the difference between pooled and single-donor products?
Pooled products combine material from multiple donors, which averages out donor-to-donor variation and gives large, uniform lots. Single-donor products come from one donor: their properties trace to that donor and can differ more between lots. Studies that need a single, well-characterized source, such as one complement lot carried through a clinical trial, use single-donor material.
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How do I reconstitute lyophilized human serum products?
Lyophilized human serum products may be reconstituted by bringing the vial to ambient temperature then resuspending in sterile water equal to the volume on the product label. For lyophilized complement products, resuspending in a volume less than the label volume will increase complement potency, useful in applications requiring increased complement activity.
