Human Serum, Single Donor, Heat Inactivated, IgG Depleted, Lyophilized

Name: Human Serum, Single Donor, Heat Inactivated, IgG Depleted, Lyophilized
Brand: Pel-Freez Biologicals
SKU: 34047L-2
Price: 64.0 USD
Availability: InStock

Human serum from individual donor units is depleted of IgG antibodies, 0.2 µm filtered, aliquoted, heat-inactivated, frozen, and lyophilized.

Specifications

Physical State:

Lyophilized Powder

Storage:

-20°C or below

Release criteria

Complement activity (CH50):

Negative

Residual IgG:

< 10%

Moisture:

< 5%

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Size:

Product code: SHUSDHIGDLY-2ML

Legacy code: 34047L-2

Quantity:

Custom/Bulk request

See bulk requirements

Bulk requests: more than 1 L

Individual donors:

Each vial is from one person. Set how many unique donors you need: 5 vials from 5 donors, or 10 vials from 5 donors.

Unit price$64.00

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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Species:	Human
Product Types:	Serum
Source:	OTC Serum
Pooling:	Single Donor
Donor Sex:	Mixed
Depletion Process:	IgG Depleted
Processing Method:	Heat Inactivated
Physical State:	Lyophilized Powder
Application Notes:	Suitable for sample dilution, immunoassays, control serum, and various applications requiring serum supplementation.
Testing:	CH50: Negative Residual IgG: <10% Moisture: <5% Serum tested negative for infectious disease per FDA guidelines.
Storage Conditions:	-20°C or below
Shipping Conditions:	Cool packs
Packaging:	Glass vial
Expiration:	1 year from date of receipt when stored dry at -20°C or below; reconstitute just before use and freeze any remainder at -70°C or below. Product quality is guaranteed to meet Pel-Freez Biologicals' specifications for this period when stored as indicated.

Product Applications

Serum bactericidal assay
Opsonophagocytic assay
Complement-dependent cytotoxicity
Hemolytic complement assay
Assay diluent & negative matrix
Assay controls & calibrators
Toxicology assays
Vaccine development
In vitro diagnostics

Documents & Datasheets

Certificate of Analysis

Find Certificate of Analysis

Frequently Asked Questions

What is complement activity (CH50 / hemolytic titer) and how is it tested?

The hemolytic titer measures how well complement lyses antibody-sensitized sheep red blood cells; CH50 is the dilution of serum that lyses 50% of the cells. It is the release test for every lot of our complement products, and our heat-inactivated human products are CH50 tested to confirm that complement activity is absent.

How is Pel-Freez Biologicals' antibody-depleted complement made?

We have developed a proprietary process for antibody removal based on affinity chromatography. It removes the immunoglobulins while maintaining high complement potency; the residual-antibody specification is given in the next question.

What's the difference between antibody-depleted human complement (ADHC), heat-inactivated ADHC (ADHC-HI) and antibody-depleted human serum (ADHS)?

ADHC (antibody-depleted human complement) keeps high complement potency for assays that need functional complement.
ADHC-HI is the same material heat-inactivated and CH50 tested to confirm no complement activity, for applications that need the other serum components but not complement.
ADHS (antibody-depleted human serum) starts from normal human serum that was not collected to preserve complement; it is neither heat-inactivated nor CH50 tested, and serves as a negative matrix for dilution, QC samples, or cell culture where complement would inhibit growth.

Which immunoglobulins are removed from your antibody-depleted human products?

We offer IgG-depleted, IgG/IgM-depleted and IgG/IgM/IgA-depleted versions of both our human complement and our human serum, all made by the same affinity chromatography process. Match the depletion to the isotypes your assay detects.

Are there residual antibodies in Pel-Freez Biologicals' antibody-depleted complement?

Our specification for residual antibody in antibody-depleted products is <3.5% of the feed serum for each isotype. Typical residual IgG is <2% and IgM is usually undetectable after depletion. Because assay conditions vary widely, many customers sample one or more lots to confirm they suit their specific system.

How is antibody-depleted human serum used as an assay control or matrix?

With the immunoglobulins removed, antibody-depleted serum is a low-background matrix for diluting samples, preparing QC and calibrator samples, and for add-back experiments where the removed isotype is spiked back in to confirm recovery.

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What is the difference between pooled and single-donor products?

Pooled products combine material from multiple donors, which averages out donor-to-donor variation and gives large, uniform lots. Single-donor products come from one donor: their properties trace to that donor and can differ more between lots. Studies that need a single, well-characterized source, such as one complement lot carried through a clinical trial, use single-donor material.

What is heat-inactivated serum and when should I use it?

Heat-inactivated serum has been held at 56°C for 30 minutes to destroy complement activity. Use it when you need the other serum components without functional complement, for example as a cell culture supplement or an assay matrix where active complement could lyse cells or cause non-specific killing.

How do I reconstitute lyophilized human serum products?

Lyophilized human serum products may be reconstituted by bringing the vial to ambient temperature then resuspending in sterile water equal to the volume on the product label. For lyophilized complement products, resuspending in a volume less than the label volume will increase complement potency, useful in applications requiring increased complement activity.

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Product References

Salic acid protects nontypeable Haemophilus influenza from natural IgM and promotes survival in murine respiratory tract.
Jackson, M. D., Wong, S. M. & Akerley, B. J. Sialic acid protects nontypeable haemophilus influenzae from natural igm and promotes survival in murine respiratory tract. Infect. Immun. 89, (2021).
Development of Complement Factor H–Based Immunotherapeutic Molecules in Tobacco Plants Against Multidrug-Resistant Neisseria gonorrhoeae
Shaughnessy, J. et al. Development of Complement Factor H–Based Immunotherapeutic Molecules in Tobacco Plants Against Multidrug-Resistant Neisseria gonorrhoeae. Front. Immunol. 11, 2680 (2020).
Synthetic DNA Delivery of an Optimized and Engineered Monoclonal Antibody Provides Rapid and Prolonged Protection against Experimental Gonococcal Infection
Parzych, E. M. et al. Synthetic dna delivery of an optimized and engineered monoclonal antibody provides rapid and prolonged protection against experimental gonococcal infection. MBio 12, 1–13 (2021).
Neisseria meningitidis urethritis outbreak isolates express a novel factor H binding protein variant that is a potential target of group B-directed meningococcal (MenB) …
Tzeng, Y. L. et al. Neisseria meningitidis urethritis outbreak isolates express a novel factor H binding protein variant that is a potential target of group B-Directed meningococcal (menb) vaccines. Infect. Immun. 88, (2020).
Vaccination with Detoxified Leukocidin AB Reduces Bacterial Load in a Staphylococcus aureus Minipig Deep Surgical Wound Infection Model
Fernandez, J. et al. Vaccination with Detoxified Leukocidin AB Reduces Bacterial Load in a Staphylococcus aureus Minipig Deep Surgical Wound Infection Model . J. Infect. Dis. (2021).
Preclinical efficacy of a lipooligosaccharide peptide mimic candidate gonococcal vaccine
Gulati, S. et al. Preclinical efficacy of a lipooligosaccharide peptide mimic candidate Gonococcal vaccine. MBio 10, (2019).
Complement-Dependent Serum Bactericidal Assays for Neisseria gonorrhoeae
Gulati, S., Rice, P. A. & Ram, S. Complement-Dependent Serum Bactericidal Assays for Neisseria gonorrhoeae. in Methods in Molecular Biology vol. 1997 267–280 (Humana Press Inc., 2019).
Development of Complement Factor H–Based Immunotherapeutic Molecules in Tobacco Plants Against Multidrug-Resistant Neisseria
Shaughnessy, J. et al. Development of Complement Factor H–Based Immunotherapeutic Molecules in Tobacco Plants Against Multidrug-Resistant Neisseria gonorrhoeae. Front. Immunol. 11, (2020).

Alternate Names

Gamma Globulin Depleted Single Donor Human Serum Heat Inactivated Lyophilized; Immunoglobulin G Depleted Single Donor Human Serum Heat Inactivated Lyophilized