Human Serum, Factor H Depleted
Normal human serum with factor H removed by immunoaffinity chromatography. Supplied with 0.1 mM EDTA to inhibit spontaneous activation; the alternative pathway is functional only when a controlling factor such as factor H is added before metal ions (Mg²⁺).
Specifications
Physical State:
Frozen Liquid
Storage:
-70°C or below
Buffer:
10 mM sodium phosphate, 145 mM NaCl, pH 7.3
Preservative:
None
Filtration:
0.22 µm
Release criteria
Protein Concentration:
>50 mg/mL
Activity:
>80% of normal human serum when reconstituted with factor H (0.5 mg/mL)
Purity:
No factor H detectable by immunodiffusion
CH50/mL Factor H-Dpl reconstituted with 500 µg Factor H/mL:
> 75 CH50 Units/mL
APH50/mL Factor H-Dpl reconstituted with 500 µg Factor H/mL:
> 70 APH50 Units/mL
HBsAg 3.0:
Negative
7 more criteria Show less
Anti-HIV 1 and 2 Plus O:
Negative
Anti-HCV 3.0:
Negative
HIV-1/HCV/HBV by NAT:
Negative
West Nile Virus (WNV) by NAT:
Negative
Anti-Syphilis:
Negative
Anti-HTLV I/II:
Negative
Factor H (1 mg/ml) + 50 µL GVBo) total reaction volume of 1.5 mL GVB++.:
50% lysis of 1 x 108 EA when incubated
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Species: | Human |
|---|---|
| Product Types: | Serum |
| Source: | Normal Human Serum |
| Pooling: | Pooled |
| Donor Sex: | Mixed |
| Depletion Process: | Immunoaffinity Chromatography |
| Processing Method: | Affinity Depleted |
| Physical State: | Frozen Liquid |
| Buffer: | 10 mM sodium phosphate, 145 mM NaCl, pH 7.3 |
| Protein Concentration: | >50 mg/mL |
| Activity: | >80% of normal human serum when reconstituted with factor H (0.5 mg/mL) |
| Purity: | No factor H detectable by immunodiffusion |
| Preservative: | None |
| Filtration: | 0.22 µm |
| Application Notes: | Used to assess ability of factor H or similar regulatory proteins to stabilize the alternative pathway stabilization system and yield a functional alternative complement pathway. |
| Testing: | Serum tested negative for infectious disease per FDA guidelines. |
| Storage Conditions: | -70°C or below |
| Shipping Conditions: | Dry ice |
| Packaging: | Cryovial |
| Expiration: | 1 year from date of receipt when stored continuously at -70°C or below; avoid repeated freeze/thaw. Product quality is guaranteed to meet Pel-Freez Biologicals' specifications for this period when stored as indicated. |
Product Applications
- Hemolytic complement assay
- Classical pathway assay
- Alternative pathway assay
- Assay diluent & negative matrix
- Assay controls & calibrators
- In vitro diagnostics
Documents & Datasheets
Certificate of Analysis
Frequently Asked Questions
What are C1q- and factor-depleted human sera used for?
Each depleted serum has a single complement component (C1q, C1r, C1s, C2 through C9, or factor B, D, H, I or P) removed, so the affected pathway stalls until that component is added back. They are used to study the role of one component, as reagents in functional complement assays, and as controls in add-back experiments.
