Human Serum, Factor H/D Depleted
Normal human serum with factors H and D removed by immunoaffinity chromatography.
Specifications
Physical State:
Frozen Liquid
Storage:
-70°C or below
Buffer:
PBS, 0.4 mM EDTA, pH 7.2
Preservative:
0.02% azide
Filtration:
0.22 µm
Release criteria
Protein Concentration:
>35 mg/mL
Activity:
Alternative pathway >60% and classical pathway >80% of normal human serum when reconstituted with factor D (2 µg/mL) and factor H (0.5 mg/mL)
Purity:
No factor H or factor D detectable by immunodiffusion
APH50/mL Factor H&D-Dpl Not reconstituted with Factor H or D:
< 1 APH50 Units/mL
APH50/mL Factor H&D-Dpl reconstituted with Factor D (2µg Factor D/mL) with Factor H (500ug Factor H/mL):
> 70 APH50 Units/mL
CH50/mL Factor H&D-Dpl Not reconstituted with Factor H or D:
≥ 75 CH50 Units/mL
11 more criteria Show less
CH50/mL Factor H&D-Dpl reconstituted with Factor D (2µg Factor D/mL) with Factor H (500ug Factor H/mL):
> 75 CH50 Units/mL
Anti-HBc:
Negative
HBsAg:
Negative
Anti-HIV 1 and 2 Plus O:
Negative
Anti-HTLV 1 and 2:
Negative
HCV RNA by NAT:
Negative
HIV RNA by NAT:
Negative
HBV DNA by NAT:
Negative
Anti-Syphilis:
Negative
WNV RNA by NAT:
Negative
Chagas:
Negative
Lead time on this item is currently: 2-4 Weeks
Need help? Ask a Specialist Contact UsOR +1 (800) 643-3426
Product Overview
| Species: | Human |
|---|---|
| Product Types: | Serum |
| Source: | Normal Human Serum |
| Pooling: | Pooled |
| Donor Sex: | Mixed |
| Depletion Process: | Immunoaffinity Chromatography |
| Processing Method: | Affinity Depleted |
| Physical State: | Frozen Liquid |
| Buffer: | PBS, 0.4 mM EDTA, pH 7.2 |
| Protein Concentration: | >35 mg/mL |
| Activity: | Alternative pathway >60% and classical pathway >80% of normal human serum when reconstituted with factor D (2 µg/mL) and factor H (0.5 mg/mL) |
| Purity: | No factor H or factor D detectable by immunodiffusion |
| Preservative: | 0.02% azide |
| Filtration: | 0.22 µm |
| Application Notes: | Used to assess C1q hemolytic activity in samples and to supply an alternative pathway activating system that is incapable of activating the classical complement pathway. |
| Testing: | Serum tested negative for infectious disease per FDA guidelines. |
| Storage Conditions: | -70°C or below |
| Shipping Conditions: | Dry ice |
| Packaging: | Cryovial |
| Expiration: | 1 year from date of receipt when stored continuously at -70°C or below; avoid repeated freeze/thaw. Product quality is guaranteed to meet Pel-Freez Biologicals' specifications for this period when stored as indicated. |
Product Applications
- Hemolytic complement assay
- Classical pathway assay
- Alternative pathway assay
- Assay diluent & negative matrix
- Assay controls & calibrators
- In vitro diagnostics
Documents & Datasheets
Certificate of Analysis
Frequently Asked Questions
What are C1q- and factor-depleted human sera used for?
Each depleted serum has a single complement component (C1q, C1r, C1s, C2 through C9, or factor B, D, H, I or P) removed, so the affected pathway stalls until that component is added back. They are used to study the role of one component, as reagents in functional complement assays, and as controls in add-back experiments.
