Human Serum, C4/Factor B Depleted
Normal human serum with C4 and factor B removed by immunoaffinity chromatography.
Specifications
Physical State:
Frozen Liquid
Storage:
-70°C or below
Complement Pathways:
<5% classical and alternative pathway activity
Buffer:
10 mM sodium phosphate, 145 mM NaCl, pH 7.3
Preservative:
None
Filtration:
0.22 µm
Release criteria
Protein Concentration:
>50 mg/mL
Activity:
>70% of normal human serum when reconstituted with C4 (0.4 mg/mL) and factor B (0.2 mg/mL)
Purity:
No C4 or factor B detectable by double immunodiffusion
Un-constituted C4/FB-Dpl:
< 10 CH50/mL
Reconstituted C4/FB-Dpl (+ 0.4mg C4 & 0.2mg FB/mL):
> 60 CH50/mL
Reconstituted C4/FB-Dpl (+ 0.40mg C4 & 0.2 mg FB/mL):
> 50 AP50/mL
10 more criteria Show less
Complement C4/FB-Depleted Human Serum,:
2 (Continued)
Anti-HBc Negative:
Negative
HBsAg Negative:
Negative
Anti-HCV Negative:
Negative
Anti-HIV 1 and 2 Plus O Negative:
Negative
HTLV I/II Negative:
Negative
HIV-1/HCV/HBV by NAT Negative:
Negative
Anti-Syphilis Negative:
Negative
West Nile Virus (WNV) by NAT Negative:
Negative
Chagas Negative:
Negative
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Species: | Human |
|---|---|
| Product Types: | Serum |
| Source: | Normal Human Serum |
| Pooling: | Pooled |
| Donor Sex: | Mixed |
| Depletion Process: | Immunoaffinity Chromatography |
| Processing Method: | Affinity Depleted |
| Physical State: | Frozen Liquid |
| Buffer: | 10 mM sodium phosphate, 145 mM NaCl, pH 7.3 |
| Protein Concentration: | >50 mg/mL |
| Activity: | >70% of normal human serum when reconstituted with C4 (0.4 mg/mL) and factor B (0.2 mg/mL) |
| Complement Pathways: | <5% classical and alternative pathway activity |
| Purity: | No C4 or factor B detectable by double immunodiffusion |
| Preservative: | None |
| Filtration: | 0.22 µm |
| Application Notes: | Minimal ability to activate the classical, alternative, and lectin complement pathways. |
| Testing: | Serum tested negative for infectious disease per FDA guidelines. |
| Storage Conditions: | -70°C or below |
| Shipping Conditions: | Dry ice |
| Packaging: | Cryovial |
| Expiration: | 1 year from date of receipt when stored continuously at -70°C or below; avoid repeated freeze/thaw. Product quality is guaranteed to meet Pel-Freez Biologicals' specifications for this period when stored as indicated. |
Product Applications
- Hemolytic complement assay
- Classical pathway assay
- Alternative pathway assay
- Assay diluent & negative matrix
- Assay controls & calibrators
- In vitro diagnostics
Documents & Datasheets
Certificate of Analysis
Frequently Asked Questions
What are C1q- and factor-depleted human sera used for?
Each depleted serum has a single complement component (C1q, C1r, C1s, C2 through C9, or factor B, D, H, I or P) removed, so the affected pathway stalls until that component is added back. They are used to study the role of one component, as reagents in functional complement assays, and as controls in add-back experiments.
