Human Serum, C1q Depleted
Normal human serum with C1q removed by immunoaffinity chromatography.
Specifications
Physical State:
Frozen Liquid
Storage:
-70°C or below
Complement Pathways:
Alternative pathway functional; lectin pathway not tested
Buffer:
10 mM sodium phosphate, 145 mM NaCl, pH 7.3
Preservative:
None
Filtration:
0.22 µm
Release criteria
Protein Concentration:
>50 mg/mL
Activity:
>80% of normal human serum when reconstituted with C1q (70 µg/mL)
Purity:
No C1q detectable by immunodiffusion
CH50/mL C1q-Dpl reconstituted with 100 µg C1q/mL:
> 40 CH50/mL
APH50/mL C1q-Dpl:
> 50 APH50/mL
HBsAg:
Negative
8 more criteria Show less
Anti-HBc antibodies:
Negative
Anti-HIV-I and 2 plus O antibodies:
Negative
Anti-HCV antibodies:
Negative
Anti-Syphilis antibodies:
Negative
HCV by NAT:
Negative
HIV by NAT:
Negative
HTLV 1 and 2:
Negative
West Nile Virus (WNV):
Negative
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Species: | Human |
|---|---|
| Product Types: | Serum |
| Source: | Normal Human Serum |
| Pooling: | Pooled |
| Donor Sex: | Mixed |
| Depletion Process: | Immunoaffinity Chromatography |
| Processing Method: | Affinity Depleted |
| Physical State: | Frozen Liquid |
| Buffer: | 10 mM sodium phosphate, 145 mM NaCl, pH 7.3 |
| Protein Concentration: | >50 mg/mL |
| Activity: | >80% of normal human serum when reconstituted with C1q (70 µg/mL) |
| Complement Pathways: | Alternative pathway functional; lectin pathway not tested |
| Purity: | No C1q detectable by immunodiffusion |
| Preservative: | None |
| Filtration: | 0.22 µm |
| Application Notes: | Used to assess C1q hemolytic activity in samples and to supply an alternative pathway activating system that is incapable of activating the classical complement pathway. |
| Testing: | Serum tested negative for infectious disease per FDA guidelines. |
| Storage Conditions: | -70°C or below |
| Shipping Conditions: | Dry ice |
| Packaging: | Cryovial |
| Expiration: | 1 year from date of receipt when stored continuously at -70°C or below; avoid repeated freeze/thaw. Product quality is guaranteed to meet Pel-Freez Biologicals' specifications for this period when stored as indicated. |
Product Applications
- Hemolytic complement assay
- Classical pathway assay
- Alternative pathway assay
- Assay diluent & negative matrix
- Assay controls & calibrators
- In vitro diagnostics
- Classical pathway reconstitution and control studies
- Immune complex and complement activation assays
Documents & Datasheets
Certificate of Analysis
Frequently Asked Questions
What are C1q- and factor-depleted human sera used for?
Each depleted serum has a single complement component (C1q, C1r, C1s, C2 through C9, or factor B, D, H, I or P) removed, so the affected pathway stalls until that component is added back. They are used to study the role of one component, as reagents in functional complement assays, and as controls in add-back experiments.
