Sheep Anti-P-Dynamin (Ser774)
Sheep polyclonal antibody to mouse and rat Dynamin (Ser774), antigen affinity purified from pooled serum.
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Sheep |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide from residues around Ser774 of rat dynamin, conjugated to keyhole limpet hemocyanin (KLH). |
| Immunogen Species: | Rat |
| Species Reactivity: | Mouse, Rat |
| Gene Name: | DNM1 |
| Molecular Weight: | 95 kDa |
| RRID: | AB_2492082 |
| Purification: | Antigen affinity-purified from pooled serum |
| Physical State: | Liquid |
| Buffer: | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol |
| Specificity: | Recognizes endogenous ~95 kDa dynamin protein phosphorylated at Ser774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by λ-phosphatase treatment. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; WB Brain 1:1000; ICC 1:1000. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)