Rat Anti-P-NLRC4 (Ser533)
Antigen affinity purified rat monoclonal antibody to human, mouse and rat NLRC4 (Ser-533).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rat |
|---|---|
| Clonality: | Monoclonal |
| Clone: | M549 |
| Immunogen: | Clone M549 was generated from a phospho-NLRC4 (Ser-533) synthetic peptide (conjugated to carrier) matching residues around serine 533 in mouse NLRC4. This sequence has high homology to human and rat NLRC4, and is not homologous to sequences in other NLR family members. |
| Immunogen Species: | Mouse |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | NLRC4 |
| Molecular Weight: | 110 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | This antibody antibody detects a 110 kDa* protein on Western blots of PMA-differentiated THP1 human macrophages. In addition, the antibody detects a mouse recombinant phospho-NLRC4 (Ser-533) peptide, but does not detect this peptide after lambda phosphatase treatment. |
| Application Notes: | Recommended starting dilution: WB 1:500. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Cancer research