Rabbit Anti-Sphingosine Kinase 2 N-Terminus
Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat Sphingosine Kinase 2 (N-terminal region).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | SK2 (N-terminal region) synthetic peptide (conjugated to KLH) matching residues in the N-terminal region of human SK2. This peptide sequence is highly conserved in rat and mouse SK2 proteins, and has no homology to SK1. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | SPHK2 |
| Molecular Weight: | 70 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | Detects 70 kDa* proteins matching the molecular mass of SK2 on Western blots of human recombinant SK2 and endogenous SK2 in human HeLa cells. The antibody also works for ELISA, immunoprecipitation, and immunocytochemistry. |
| Application Notes: | Recommended starting dilutions: WB 1:250; ICC 1:100; IP 1:100. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Immunoprecipitation
- Cancer research