Rabbit Anti-Sox2 (Lys-119), Methyl-Specific

Antigen affinity purified rabbit polyclonal antibody to chicken, human, mouse, rat and Xenopus Sox2 (Lys-119).

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: ARBS20V3-100UL

Unit price$348.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Methylated-Sox2 (Lys-119) synthetic peptide (conjugated to carrier) matching residues around Lys-119 in mouse Sox2. The sequence is conserved in rat and human Sox2, and has high homology with the conserved site in Sox1 (Lys-127) and Sox3 (Lys-145), but low homology to other Sox family members.
Immunogen Species: Mouse
Species Reactivity: Chicken, Frog, Human, Mouse, Rat
Gene Name: SOX2
Molecular Weight: 34
Purification: Antigen affinity-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: The antibody was cross adsorbed to unmethylated Sox2 (Lys-119) peptide before affinity purification using methylated-Sox2 (Lys-119) peptide. This antibody antibody detects a 34 kDa* protein on Western blots of mouse F9 stem cells, as well as human Sox2 recombinant protein that was methylated by Set7 methyltransferase.
Application Notes: Recommended starting dilutions: WB 1:1000; ICC 1:100.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunofluorescence (IF/ICC)
  • Cancer research
Documents & Datasheets

Certificate of Analysis