Rabbit Anti-Plexin A1 (Sema Domain)

Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat Plexin A1 (Sema Domain).

Rabbit Anti-Plexin A1 (Sema Domain)
Rabbit Anti-Plexin A1 (Sema Domain)
Western blots showing Cos-7 cells transfected with mouse Myc-tagged Plexin-A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin-A1 immunoprecipitated from Myc-tagged Plexin-A1 transfected cells using anti-Myc (lane 4) or anti-Plexin-A1 (lane 5). These blots were probed with either the affinity purified anti-Plexin-A1 (lanes 1 & 2) or with mouse monoclonal anti-Myc (lanes 3-5).
Western blots showing Cos-7 cells transfected with mouse Myc-tagged Plexin-A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin-A1 immunoprecipitated from Myc-tagged Plexin-A1 transfected cells using anti-Myc (lane 4) or anti-Plexin-A1 (lane 5). These blots were probed with either the affinity purified anti-Plexin-A1 (lanes 1 & 2) or with mouse monoclonal anti-Myc (lanes 3-5).
Immunocytochemical double labeling using anti-Myc mouse monoclonal and anti-Plexin-A1 rabbit polyclonal antibodies in Cos-7 cells mock transfected (A,D) or transfected with Myc-tagged mouse Plexin-A1 construct (B,E). The specificity of the binding in E was demonstrated by using Plexin-A1 peptide in the presence of this anti-Plexin-A1 antibody (C,F).
Immunocytochemical double labeling using anti-Myc mouse monoclonal and anti-Plexin-A1 rabbit polyclonal antibodies in Cos-7 cells mock transfected (A,D) or transfected with Myc-tagged mouse Plexin-A1 construct (B,E). The specificity of the binding in E was demonstrated by using Plexin-A1 peptide in the presence of this anti-Plexin-A1 antibody (C,F).

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: ARBPAD-100UL

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Unit price$348.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Plexin-A1 synthetic peptide (conjugated to carrier protein) matching a region within the N-terminal half of the semaphorin domain in human Plexin-A1. This sequence is highly conserved in rat and mouse Plexin-A1, and has low homology to other Plexin family members.
Immunogen Species: Human
Species Reactivity: Human, Mouse, Rat
Gene Name: PLXNA1
Molecular Weight: 220
Purification: Antigen affinity-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: Detects a 220 kDa* protein matching the apparent molecular mass of Plexin-A1 on Western blots of neonatal rat brain and Cos-7 cells transfected with mouse Plexin-A1. In immunocytochemistry, this antibody co-localizes with anti-Myc in Cos-7 cells transfected with Myc-tagged mouse Plexin-A1.
Application Notes: Recommended starting dilutions: WB 1:1000; IHC 1:100; ICC 1:100; IP 1:50.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Immunoprecipitation
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis