Rabbit Anti-P-VE-Cadherin (Tyr685)

Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat VE-Cadherin (Tyr-685).

Rabbit Anti-P-VE-Cadherin (Tyr685)
Rabbit Anti-P-VE-Cadherin (Tyr685)
Western blot image of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. then the blots were untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-VE-cadherin (Tyr-685) (lanes 1 & 2) or mouse monoclonal anti-VE-cadherin (lanes 3 & 4).
Western blot image of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. then the blots were untreated (lanes 1 & 3) or treated with alkaline phosphatase (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-VE-cadherin (Tyr-685) (lanes 1 & 2) or mouse monoclonal anti-VE-cadherin (lanes 3 & 4).
Phosphorylation of CDH5 Y658, CDH5 Y685 and VEGFR2 (Tyr1054/1059) in cultured human LECs after Yoda1 (0.3 µM) treatment for the indicated periods. Image from publication CC-BY-4.0. PMID: 38528202
Phosphorylation of CDH5 Y658, CDH5 Y685 and VEGFR2 (Tyr1054/1059) in cultured human LECs after Yoda1 (0.3 µM) treatment for the indicated periods. Image from publication CC-BY-4.0. PMID: 38528202

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: ARBCHJ-100UL

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Unit price$348.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Phospho-VE-Cadherin (Tyr-685) synthetic peptide (conjugated to carrier protein) matching residues around tyrosine 685 in human VE-cadherin. This sequence has significant homology to the conserved site in rat and mouse VE-cadherin, but is not conserved in other cadherins.
Immunogen Species: Human
Species Reactivity: Human, Mouse, Rat
Gene Name: CDH5
Molecular Weight: 140
Purification: Antigen affinity-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: The antibody was cross-adsorbed to an unrelated phospho-tyrosine peptide and unphosphorylated VE-cadherin (Tyr-685) peptide before affinity purification using phospho-VE-cadherin (Tyr-685) peptide. The purified antibody detects a 140 kDa* band matching VE-cadherin in western blots of human endothelial cells treated with pervanadate, and this band is not detected in untreated cells or after alkaline phosphatase treatment.
Application Notes: Recommended starting dilution: WB 1:1000.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Cancer research
Documents & Datasheets

Certificate of Analysis