Rabbit Anti-Phospho-Tyrosine Hydroxylase (Ser40) Antibody
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in dopamine and norepinephrine synthesis, so TH antibodies mark dopaminergic and noradrenergic neurons in studies of depression, schizophrenia, Parkinson's disease and drug abuse, and probe catecholamine signaling generally. TH activity is regulated by phosphorylation; phospho-specific antibodies to its phosphorylation sites are used to study this regulation and to identify the cells in which it occurs.
Specifications
Physical State:
Liquid
Storage:
-20°C Short term, -80°C Long term
Buffer:
Learn more 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50% Glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide from residues around Ser40 of rat tyrosine hydroxylase, conjugated to keyhole limpet hemocyanin (KLH). |
| Immunogen Species: | Rat |
| Gene Name: | TH |
| Molecular Weight: | 60 kDa |
| RRID: | AB_2492279 |
| Purification: | Antigen affinity-purified from pooled serum on sequential phospho- and non-phosphopeptide affinity columns |
| Physical State: | Liquid |
| Buffer: | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50% Glycerol |
| Specificity: | Recognizes endogenous ~60 kDa tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over non-phospho TH. |
| Application Notes: | Recommended starting dilution: WB, IF, IHC 1:1000 |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C Short term, -80°C Long term |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | 1 year from date of receipt when stored at -20°C (short term) or -80°C (long term); aliquot and avoid repeated freeze/thaw. Product quality is guaranteed to meet Pel-Freez Biologicals' specifications for this period when stored as indicated. |
Product Applications
- Western blot
- Immunohistochemistry
- Immunofluorescence (IF/ICC)
- Neuroscience research
Documents & Datasheets
Datasheet
Safety Datasheet
Certificate of Analysis
Frequently Asked Questions
What dilution should I use for your antibodies in western blot, ELISA or formalin-fixed paraffin-embedded tissue?
A typical starting dilution for antibodies in immunoassays is 1:1000. Consider adjusting the dilution depending on your experimental conditions, to increase or decrease the signal-to-noise ratio, etc.
Should I store antibodies at -20°C or -80°C, and should I aliquot them on delivery?
Either is fine, but avoid repeated freeze-thaw cycles. Our antibodies contain glycerol, so they do not freeze at -20°C but do at -80°C. If you will use the antibody within a few weeks, store it at -20°C. For long-term storage, split it into single-use aliquots and store at -80°C.
Alternate Names
TH; TYH; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase
