Rabbit Anti-P-PTP1B (Tyr152)
Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat PTP1B (Tyr-152).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | PTP1B (Tyr-152) synthetic peptide corresponds to residues around Tyr-152 in human PTP1B. This sequence has significant homology to the conserved site in rat and mouse PTP1B, and has homology to conserved sites in TC-PTP and MPTP, but not other protein tyrosine phosphatase family members. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | PTPN1 |
| Molecular Weight: | 50 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | The antibody was cross-adsorbed to a phospho-tyrosine peptide then affinity purified with phospho-PTP1B (Tyr-152) peptide. The purified antibody detects a 50 kDa* band matching PTP1B in western blots of pervanadate treated human A431 and Jurkat, as well as mouse SYF cells. This reactivity is not observed in control cells or after alkaline phosphatase treatment of blots. |
| Application Notes: | Recommended starting dilution: WB 1:1000. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Cancer research