Rabbit Anti-P-PDK1 (Tyr9)
Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat PDK1 (Tyr-9).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Phospho-PDK1 (Tyr-9) synthetic peptide (conjugated to carrier protein) corresponds to residues around tyrosine 9 in human PDK1. This sequence is highly conserved in rat and mouse PDK1. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | PDK1 |
| Molecular Weight: | 65 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | The antibody was cross-adsorbed to phospho-tyrosine coupled to agarose, then affinity purified using phospho-PDK1 (Tyr-9) peptide. The purified antibody detects a 65 kDa* protein matching the apparent molecular mass of PDK1 on Western blots of human A431 cells treated with EGF, IGF-1, or pervanadate, but not in untreated cells. PDK1 (Tyr-9) reactivity was also observed in adult mouse brain. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:200. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Cancer research