Rabbit Anti-P-MEK1/2 (Ser218,222)
Rabbit polyclonal antibody to mouse and human MEK1/2 (Ser218,222), antigen affinity purified from pooled serum.
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide from residues around Ser218/222 of human MEK1/2, conjugated to keyhole limpet hemocyanin (KLH). Note: The phosphosites in human MEK 1/2 are Ser218 and Ser222; the phosphosites in mouse MEK 1/2 are Ser217 and Ser221. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse |
| Gene Name: | MAP2K1/2 |
| Molecular Weight: | 45 kDa |
| RRID: | AB_2492146 |
| Purification: | Antigen affinity-purified from pooled serum on sequential phospho- and non-phosphopeptide affinity columns |
| Physical State: | Liquid |
| Buffer: | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol |
| Specificity: | Recognizes endogenous ~45 kDa MEK 1/2 protein phosphorylated at Ser218/222. Labeling is abolished by pre-adsorption with the phosphopeptide used as antigen, and not by the matching non-phosphopeptide. |
| Application Notes: | Recommended starting dilution: WB 1:1000. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Cancer research