Rabbit Anti-P-MeCP2 (Ser421)
Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat MeCP2 (Ser-421).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Phospho-MeCP2 (Ser-421) synthetic peptide (conjugated to KLH) matching residues around serine 421 in mouse MeCP2. This peptide sequence is highly conserved in rat and human MeCP2, and has low homology to other nuclear proteins. |
| Immunogen Species: | Mouse |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | MECP2 |
| Molecular Weight: | 75 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | Detects a 75 kDa* protein matching the molecular mass of MeCP2 on Western blots of human PC3 cells treated with calyculin A and in mouse brain tissue. These reactivities are not observed after lambda phosphatase treatment. |
| Application Notes: | Recommended starting dilutions: WB 1:500; ICC 1:250. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Neuroscience research