Rabbit Anti-P-iNOS (Tyr1055)
Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat iNOS (Tyr-1055).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Phospho-iNOS (Tyr-1055) synthetic peptide (conjugated to carrier protein) corresponds to residues around Tyr-1055 in human iNOS. The sequence is conserved in rat and mouse iNOS, and has some homology to the conserved sites in eNOS (Tyr-1087) and nNOS (Tyr-1326). |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | NOS2 |
| Molecular Weight: | 130 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | Detects a 130 kDa* protein matching induced NOS on Western blots of mouse macrophages treated with LPS followed by pervanadate. The antibody also detects a 150 kDa doublet that corresponds with higher molecular weight forms of NOS, which are also observed using other anti-iNOS antibodies. In addition, the antibody detects a non-specific 120 kDa band that has not been identified. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:400. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Cancer research